cDNA Clone MKK5

• Plasmid: pGEX-MKK5 FL 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-MKK5 FL 6His
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGAGAACCAGGTGCTGGTAATTCGCATCAAGATCCCAAATAGTGG
CGCGGTGGACTGGACAGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATG
TGCTGGATGTGATAGGCCAGGTTCTGCCTGAAGCAACAACTACAGCATTT
GAATATGAAGATGAAGATGGTGATCGAATTACAGTGAGAAGTGATGAGGA
AATGAAGGCAATGCTGTCATATTATTATTCCACAGTAATGGAACAGCAAG
TAAATGGACAGTTAATAGAGCCTCTGCAGATATTTCCAAGAGCCTGCAAG
CCTCCTGGGGAACGGAACATACATGGCCTGAAGGTGAATACTCGGGCCGG
ACCCTCTCAACACAGCAGCCCAGCAGTCTCAGATTCACTTCCAAGCAATA
GCTTAAAGAAGTCTTCTGCTGAACTGAAAAAAATACTAGCCAATGGCCAG
ATGAATGAACAAGACATACGATATCGGGACACTCTTGGTCATGGCAACGG
AGGCACAGTCTACAAAGCATATCATGTCCCGAGTGGGAAAATATTAGCTG
TAAAGGTCATACTACTAGATATTACACTGGAACTTCAGAAGCAAATTATG
TCTGAATTGGAAATTCTTTATAAGTGCGATTCATCATATATCATTGGATT
TTATGGAGCATTTTTTGTAGAAAACAGGATTTCAATATGTACAGAATTCA
TGGATGGGGGATCTTTGGATGTATATAGGAAAATGCCAGAACATGTCCTT
GGAAGAATTGCAGTAGCAGTTGTTAAAGGCCTTACTTATTTGTGGAGTTT
AAAGATTTTACATAGAGACGTGAAGCCCTCCAATATGCTAGTAAACACAA
GAGGACAGGTTAAGCTGTGTGATTTTGGAGTTAGCACTCAGCTGGTGAAT
TCTATAGCCAAGACGTATGTTGGAACAAATGCTTATATGGCGCCTGAAAG
GATTTCAGGGGAGCAGTATGGAATTCATTCTGATGTCTGGAGCTTAGGAA
TCTCTTTTATGGAGCTTGCTCTTGGGAGGTTTCCATATCCTCAGATTCAG
AAAAACCAGGGATCTTTAATGCCTCTCCAGCTTCTGCAGTGCATTGTTGA
TGAGGATTCGCCCGTCCTTCCAGTTGGAGAGTTCTCGGAGCCATTTGTAC
ATTTCATCACTCAGTGTATGCGAAAACAGCCAAAAGAAAGGCCAGCACCT
GAAGAATTGATGGGCCACCCGTTCATCGTGCAGTTCAATGATGGAAATGC
CGCCGTGGTGTCCATGTGGGTGTGCCGGGCGCTGGAGGAGAGGCGGAGCC
AGCAGGGGCCCCCGCACCATCACCATCACCATGCGGCCGC

Amino Acid Sequence

GSENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAF
EYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACK
PPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQ
MNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIM
SELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVL
GRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVN
SIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQ
KNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAP
EELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPPHHHHHH

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU435
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct with N terminal GST, C terminal 6His tag