cDNA Clone SGK3

• Plasmid: pEBG-deltaN-SGK3
• Parent Plasmid: pEBG2T
• Expressed: GST-deltaN-SGK3
• Genbank: BAD97243.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGACAGTCCAAAACACCAGTCAGATCCATCTGAAGATGAGGA
TGAAAGAAGTTCTCAGAAGCTACACTCTACCTCACAGAACATCAACCTGG
GACCGTCTGGAAATCCTCATGCCAAACCAACTGACTTTGATTTCTTAAAA
GTTATTGGAAAAGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACT
GGATGGAAAATTTTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCA
ACAGAAAAGAGCAAAAACATATTATGGCTGAACGTAATGTGCTCTTGAAA
AATGTGAAACATCCGTTTTTGGTTGGATTGCATTATTCCTTCCAAACAAC
TGAAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTTTTTT
TCCACTTACAAAGAGAACGGTCCTTTCCTGAGCACAGAGCTAGGTTTTAC
GCTGCTGAAATTGCTAGTGCATTGGGTTACTTACATTCCATCAAAATAGT
ATACAGAGACTTGAAACCAGAAAATATTCTTTTGGATTCAGTAGGACATG
TTGTCTTAACAGATTTTGGGCTTTGTAAAGAAGGAATTGCTATTTCTGAC
ACCACTACCACATTTTGTGGGACACCAGAGTATCTTGCACCTGAAGTAAT
TAGAAAACAGCCCTATGACAATACTGTAGATTGGTGGTGCCTTGGGGCTG
TTCTGTATGAAATGCTGTATGGATTGCCTCCTTTTTATTGCCGAGATGTT
GCTGAAATGTATGACAATATCCTTCACAAACCCCTAAGTTTGAGGCCAGG
AGTGAGTCTTACAGCCTGGTCCATTCTGGAAGAACTCCTAGAAAAAGACA
GGCAAAATCGACTTGGTGCCAAGGAAGACTTTCTTGAAATTCAGAATCAT
CCTTTTTTTGAATCACTCAGCTGGGCTGACCTTGTACAAAAGAAGATTCC
ACCACCATTTAATCCTAATGTGGCTGGACCAGATGATATCAGAAACTTTG
ACACAGCATTTACAGAAGAAACAGTTCCATATTCTGTGTGTGTATCTTCT
GACTATTCTATAGTGAATGCCAGTGTATTGGAGGCAGATGATGCATTCGT
TGGTTTCTCTTATGCACCTCCTTCAGAAGACTTATTTTTGTGAGCGGCCG
C

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMDSPKHQSDPSEDEDERSSQKLHS
TSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKV
LQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDF
VNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENI
LLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTV
DWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSIL
EELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAG
PDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSE
DLFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU192
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; Calc Mol Weight 69219 Da