cDNA Clone PRPF8

• Synonyms: HPRP8, PRP8, PRPC8, RP13, SNRNP220
• Plasmid: pCMV5-HA-2 PRPF8
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-PRPF8
• Genbank: NM_006445.3
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCTATGGCCGGAGTGTTTCCTTATCGAGGGCCGGGTAACCCGGTGCCTGGCCCTCTAGCCCCGCTACCGGACTACATGTCGGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAATTGCAGGCCAAGCGCTATGCAGAAAAGCGGAAGTTTGGGTTTGTGGATGCCCAGAAGGAAGACATGCCCCCAGAACATGTCAGGAAGATCATTCGAGACCATGGAGACATGACCAACAGGAAGTTCCGCCATGACAAAAGGGTTTACTTGGGTGCCCTAAAGTACATGCCCCACGCAGTCCTCAAACTCCTGGAGAACATGCCTATGCCTTGGGAGCAGATTCGGGATGTGCCTGTGCTGTACCACATCACTGGAGCCATTTCCTTCGTCAATGAGATTCCCTGGGTCATTGAACCTGTCTACATCTCCCAGTGGGGGTCAATGTGGATTATGATGCGCCGAGAAAAAAGAGATAGGAGGCATTTCAAGAGGATGCGTTTTCCCCCTTTTGATGATGAGGAGCCGCCCTTGGACTATGCTGACAACATCCTAGATGTTGAGCCACTGGAGGCCATTCAGCTAGAGCTGGACCCTGAGGAGGACGCCCCTGTGTTGGACTGGTTCTATGACCACCAGCCGTTGAGGGACAGCAGGAAGTATGTAAATGGCTCCACTTACCAGCACTGGCAGTTCACACTACCTATGATGTCGACTCTCTACCGCCTGGCTAATCAGCTCCTGACAGACTTGGTGGATGACAACTACTTCTACCTGTTTGATTTGAAGGCCTTCTTTACGTCCAAGGCACTCAATATGGCCATTCCTGGAGGCCCCAAATTTGAACCTCTTGTTCGAGACATCAACCTACAGGATGAAGACTGGAATGAATTCAATGATATTAACAAGATTATCATCCGGCAGCCTATCCGCACTGAGTACAAGATTGCTTTTCCTTACTTGTACAACAATCTTCCACACCATGTCCACCTCACCTGGTACCATACTCCCAATGTTGTATTCATCAAAACTGAGGATCCTGACTTGCCAGCTTTCTACTTTGACCCTTTGATCAACCCAATCTCCCATAGGCACTCAGTCAAGAGCCAGGAACCATTGCCGGATGATGATGAGGAATTTGAGCTCCCGGAGTTTGTGGAGCCCTTCCTGAAGGACACACCCCTCTATACAGACAATACAGCCAATGGCATTGCCCTGCTCTGGGCCCCGCGGCCCTTCAACCTACGCTCTGGTCGCACCCGTCGGGCCCTGGACATACCCCTTGTCAAGAACTGGTATCGGGAGCATTGTCCTGCCGGGCAGCCTGTGAAAGTGAGGGTCTCCTACCAGAAGCTGCTTAAGTACTATGTGCTGAATGCCCTGAAGCATCGGCCCCCTAAGGCTCAAAAGAAGAGGTATTTGTTCCGCTCCTTCAAAGCCACCAAATTCTTTCAGTCCACAAAGCTGGACTGGGTGGAGGTTGGGCTCCAGGTTTGCCGCCAGGGCTACAACATGCTCAACCTTCTCATTCACCGCAAAAACCTCAACTACCTGCACCTGGACTACAACTTCAACCTCAAGCCTGTGAAAACGCTCACCACCAAGGAAAGAAAGAAATCTCGTTTTGGGAATGCTTTCCACCTGTGTCGGGAAGTTCTGCGTTTGACTAAGCTGGTGGTGGATAGTCACGTGCAGTATCGGCTGGGCAATGTGGATGCCTTCCAGCTGGCAGATGGATTGCAGTATATATTTGCCCATGTTGGGCAGTTGACGGGCATGTATCGATACAAATACAAGCTGATGCGACAGATTCGCATGTGCAAGGACCTGAAGCATCTCATCTATTATCGTTTCAACACGGGCCCTGTAGGGAAGGGTCCTGGCTGTGGCTTCTGGGCTGCCGGTTGGCGAGTCTGGCTCTTTTTCATGCGTGGCATTACCCCTTTATTAGAGCGATGGCTTGGCAACCTCCTGGCCCGGCAGTTTGAAGGTCGACACTCAAAGGGGGTGGCAAAGACAGTAACAAAGCAGCGAGTGGAGTCACATTTTGACCTTGAGCTGCGGGCAGCTGTGATGCATGATATTCTGGACATGATGCCTGAGGGGATCAAGCAGAACAAGGCCCGGACAATCCTGCAGCACCTCAGTGAAGCCTGGCGCTGCTGGAAAGCCAACATTCCCTGGAAGGTCCCTGGGCTGCCGACGCCCATAGAGAATATGATCCTTCGATACGTGAAGGCCAAGGCTGACTGGTGGACCAACACTGCCCACTACAACCGAGAACGGATCCGCCGAGGGGCCACTGTGGACAAGACTGTTTGTAAAAAGAATCTGGGCCGCCTCACCCGGCTCTATCTGAAGGCAGAACAGGAGCGGCAGCACAACTACCTGAAGGACGGGCCTTACATCACAGCGGAGGAAGCAGTGGCAGTATATACCACCACAGTGCATTGGTTGGAAAGCCGCAGGTTTTCACCCATCCCATTCCCCCCACTCTCCTATAAGCATGACACCAAGTTGCTCATCTTGGCATTGGAGCGGCTCAAGGAAGCTTATAGTGTGAAGTCTCGGTTGAACCAGTCTCAGAGGGAGGAGCTAGGTCTGATCGAGCAGGCCTACGATAACCCCCACGAGGCGCTGTCCCGCATCAAGCGTCACCTCCTCACACAGAGAGCCTTCAAAGAGGTGGGCATTGAGTTCATGGATCTGTATAGCCACCTCGTTCCAGTATATGATGTTGAGCCCCTGGAGAAGATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCCGACAAGCGCCGCCTGTTCCCACCCTGGATTAAGCCTGCAGACACAGAACCACCTCCGCTGCTTGTTTACAAGTGGTGTCAAGGCATCAATAACCTGCAGGACGTGTGGGAGACGAGTGAAGGCGAGTGCAATGTCATGCTGGAATCCCGCTTTGAGAAGATGTATGAGAAGATCGACTTGACTCTGCTCAACAGGCTGCTGCGCCTCATCGTGGACCACAACATAGCCGACTACATGACAGCCAAGAACAACGTCGTCATCAACTATAAGGACATGAACCATACGAATTCATATGGGATCATCAGAGGCCTGCAGTTTGCCTCATTCATCGTGCAGTATTATGGCCTGGTGATGGATTTGCTTGTATTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCCCCTCAGATGCCAAATGACTTTCTCAGTTTCCAGGACATAGCCACTGAGGCTGCCCACCCCATCCGTCTCTTCTGCAGATACATTGATCGCATCCATATTTTTTTCAGGTTCACAGCAGATGAGGCTCGGGACCTGATTCAACGTTACCTGACAGAGCACCCTGACCCCAATAATGAAAACATCGTTGGCTATAATAACAAGAAGTGCTGGCCCCGAGATGCCCGCATGCGCCTCATGAAACATGATGTTAACTTAGGCCGGGCGGTATTCTGGGACATCAAGAACCGCTTGCCACGGTCAGTGACTACAGTTCAGTGGGAGAACAGCTTCGTGTCTGTGTACAGTAAGGACAACCCCAACCTGCTGTTCAACATGTGTGGCTTCGAGTGCCGCATCCTGCCTAAGTGCCGCACCAGCTATGAGGAGTTCACCCACAAGGACGGGGTCTGGAACCTGCAGAATGAGGTTACTAAGGAGCGCACAGCTCAGTGTTTCCTGCGTGTGGACGATGAGTCAATGCAGCGCTTCCACAACCGCGTGCGTCAGATTCTCATGGCCTCTGGGTCCACCACCTTCACCAAGATTGTGAATAAGTGGAATACAGCTCTCATTGGCCTTATGACATACTTTCGGGAGGCTGTGGTGAACACCCAAGAGCTCTTGGACTTACTGGTGAAGTGTGAGAACAAAATCCAGACACGTATCAAGATTGGACTCAACTCCAAGATGCCAAGTCGGTTCCCCCCGGTTGTGTTCTACACCCCTAAGGAGTTGGGTGGACTCGGCATGCTCTCAATGGGCCATGTGCTCATCCCCCAATCCGACCTCAGGTGGTCCAAACAGACAGATGTAGGTATCACACACTTTCGTTCAGGAATGAGCCATGAAGAAGACCAGCTCATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAGCGAGTTCATTGATTCTCAGCGGGTCTGGGCTGAGTACGCACTCAAGAGGCAAGAGGCCATTGCTCAGAACAGACGCCTGACTTTAGAAGACCTAGAAGATTCATGGGATCGTGGCATTCCTCGAATCAATACCCTCTTCCAGAAGGACCGGCACACACTGGCTTATGATAAGGGCTGGCGTGTCAGAACTGACTTTAAGCAGTATCAGGTTTTGAAGCAGAATCCGTTCTGGTGGACACACCAGCGGCATGATGGGAAGCTCTGGAACCTGAACAACTACCGTACAGACATGATCCAGGCCCTGGGCGGTGTGGAAGGCATTCTGGAACACACACTCTTTAAGGGCACTTACTTCCCTACCTGGGAGGGGCTTTTCTGGGAGAAGGCCAGTGGCTTTGAGGAATCTATGAAGTGGAAGAAGCTAACTAATGCTCAGCGATCAGGACTGAACCAGATTCCCAATCGTAGATTCACCCTCTGGTGGTCCCCGACCATTAATCGAGCCAATGTATATGTAGGCTTTCAGGTGCAGCTAGACCTGACGGGTATCTTCATGCACGGCAAGATCCCCACGCTGAAGATCTCTCTCATCCAGATCTTCCGAGCTCACTTGTGGCAGAAGATCCATGAGAGCATTGTTATGGACTTATGTCAGGTGTTTGACCAGGAACTTGATGCACTGGAAATTGAGACAGTACAAAAGGAGACAATCCATCCCCGAAAGTCATATAAGATGAACTCTTCCTGTGCAGATATCCTGCTCTTTGCCTCCTATAAGTGGAATGTCTCCCGGCCCTCATTGCTGGCTGACTCCAAGGATGTGATGGACAGCACCACCACCCAGAAATACTGGATTGACATCCAGTTGCGCTGGGGGGACTATGATTCCCACGACATTGAGCGCTACGCCCGGGCCAAGTTCCTGGACTACACCACCGACAACATGAGTATCTACCCTTCGCCCACAGGTGTACTCATCGCCATTGACCTGGCCTATAACTTGCACAGTGCCTATGGAAACTGGTTCCCAGGCAGCAAGCCTCTCATACAACAGGCCATGGCCAAGATCATGAAGGCAAACCCTGCCCTGTATGTGTTACGTGAACGGATCCGCAAGGGGCTACAGCTCTATTCATCTGAACCCACTGAGCCTTATTTGTCTTCTCAGAACTATGGTGAGCTCTTCTCCAACCAGATTATCTGGTTTGTGGATGACACCAACGTCTACAGAGTGACTATTCACAAGACCTTTGAAGGGAACTTGACAACCAAGCCCATCAACGGAGCCATCTTCATCTTCAACCCACGCACAGGGCAGCTGTTCCTCAAGATAATCCACACGTCCGTGTGGGCGGGACAGAAGCGTTTGGGGCAGTTGGCTAAGTGGAAGACAGCTGAGGAGGTGGCCGCCCTGATCCGATCTCTGCCTGTGGAGGAGCAGCCCAAGCAGATCATTGTCACCAGGAAGGGCATGCTGGACCCACTGGAGGTGCACTTACTGGACTTCCCCAATATTGTCATCAAAGGATCGGAGCTCCAACTCCCTTTCCAGGCGTGTCTCAAGGTGGAAAAATTCGGGGATCTCATCCTTAAAGCCACTGAGCCCCAGATGGTTCTCTTCAACCTCTATGACGACTGGCTCAAGACTATTTCATCTTACACGGCCTTCTCCCGTCTCATCCTGATTCTGCGTGCCCTACATGTGAACAACGATCGGGCAAAAGTGATCCTGAAGCCAGACAAGACTACTATTACAGAACCACACCACATCTGGCCCACTCTGACTGACGAAGAATGGATCAAGGTCGAGGTGCAGCTCAAGGATCTGATCTTGGCTGACTACGGCAAGAAAAACAATGTGAACGTGGCATCACTGACACAATCAGAAATTCGAGACATCATCCTGGGTATGGAGATCTCGGCACCGTCACAGCAGCGGCAGCAGATCGCTGAGATCGAGAAGCAGACCAAGGAACAATCGCAGCTGACGGCAACACAGACTCGCACTGTCAACAAGCATGGCGATGAGATCATCACCTCCACCACCAGCAACTATGAGACCCAGACTTTCTCATCCAAGACTGAGTGGAGGGTCAGGGCCATCTCTGCTGCCAACCTGCACCTAAGGACCAATCACATCTATGTTTCATCTGACGACATCAAGGAGACTGGCTACACCTACATCCTTCCCAAGAATGTGCTTAAGAAGTTCATCTGCATATCTGACCTTCGGGCCCAAATTGCAGGATACCTATATGGGGTGAGCCCACCAGATAACCCCCAGGTGAAGGAGATCCGCTGCATTGTGATGGTGCCGCAGTGGGGCACTCACCAGACCGTGCACCTGCCTGGCCAGCTGCCCCAGCATGAGTACCTCAAGGAGATGGAACCCTTAGGTTGGATCCACACTCAGCCCAATGAGTCCCCGCAGTTATCACCCCAGGATGTCACCACCCATGCCAAGATCATGGCTGACAACCCATCTTGGGATGGCGAGAAGACCATTATCATCACATGCAGCTTCACGCCAGGCTCCTGTACACTGACGGCCTACAAGCTGACCCCCAGTGGCTACGAATGGGGCCGCCAGAACACAGACAAGGGCAACAACCCCAAGGGCTACCTGCCTTCACACTATGAGAGGGTGCAGATGCTGCTGTCGGACCGTTTCCTTGGCTTCTTCATGGTCCCTGCCCAGTCCTCGTGGAACTACAACTTCATGGGTGTTCGGCATGACCCCAACATGAAATATGAGCTACAGCTGGCGAACCCCAAAGAGTTCTACCACGAGGTGCACAGGCCCTCTCACTTCCTCAACTTTGCTCTCCTGCAGGAGGGGGAGGTTTACTCTGCGGATCGGGAGGACCTGTATGCCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMT
NRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLD
YADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQHWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGP
KFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEE
FELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFK
ATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADG
LQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRV
ESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLG
RLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEA
LSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVML
ESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDI
ATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSV
YSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREA
VVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWES
EFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQA
LGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIF
RAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIE
RYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQII
WFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLL
DFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWI
KVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRA
ISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGW
IHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQS
SWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU33802
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains the known variation R227H which is benign according to ensembl. Also contains a silent T>C nucleotide change at 5343 bp.