cDNA Clone HECW2

• Synonyms: NDHSAL, NEDL2
• Plasmid: pcDNA5-FRT/TO-GFP HECW2
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-HECW2
• Genbank: NM_020760.3
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGGTCGACATGGCTAGTTCAGCCCGGGAGCACCTGCTTTTTGTGAGGCGT
CGAAATCCCCAGATGCGGTACACATTGAGCCCAGAGAACCTCCAGAGCCT
TGCCGCCCAGAGCTCCATGCCAGAGAACATGACCCTGCAGCGGGCCAACA
GCGACACCGACCTGGTGACTTCTGAGAGCCGCTCCAGCTTAACTGCCAGC
ATGTACGAGTACACGCTGGGGCAAGCCCAGAACCTCATTATCTTCTGGGA
CATTAAAGAGGAGGTGGACCCCAGTGATTGGATTGGACTTTATCATATAG
ATGAGAATTCTCCAGCCAACTTCTGGGATTCTAAGAACAGGGGTGTGACT
GGAACACAAAAAGGGCAAATTGTATGGAGAATTGAGCCTGGGCCCTATTT
CATGGAACCGGAGATAAAAATCTGTTTTAAATATTACCACGGCATTAGTG
GAGCCCTGCGAGCCACGACCCCCTGCATCACCGTGAAGAACCCAGCTGTG
ATGATGGGGGCAGAAGGCATGGAGGGAGGTGCTTCAGGAAACCTGCATTC
TCGAAAACTTGTTAGCTTTACATTGTCAGATCTTAGGGCAGTTGGGCTAA
AGAAAGGGATGTTCTTCAATCCTGACCCTTATCTTAAGATGTCAATTCAG
CCAGGAAAGAAGAGCAGTTTCCCCACCTGTGCCCACCACGGGCAGGAGAG
ACGGTCTACTATCATCAGTAACACCACCAATCCAATTTGGCACCGAGAGA
AATATTCCTTTTTTGCACTTCTTACTGATGTCTTAGAAATTGAAATTAAA
GACAAATTTGCCAAGAGCCGTCCCATCATCAAGCGTTTTCTGGGGAAACT
AACCATTCCAGTCCAGAGGCTGCTGGAGCGACAAGCCATCGGTGATCAAA
TGCTCAGCTACAACCTTGGCAGAAGGCTCCCAGCTGACCACGTGAGTGGG
TACCTCCAGTTTAAAGTGGAGGTTACGTCTTCTGTTCATGAAGATGCCTC
TCCAGAAGCTGTTGGCACAATACTTGGAGTCAATTCTGTGAATGGAGACT
TAGGTAGCCCTTCCGATGACGAGGACATGCCAGGGAGCCATCACGACAGC
CAGGTGTGCTCTAATGGGCCAGTTTCTGAGGACAGTGCTGCCGATGGAAC
CCCCAAGCATTCATTCAGGACTAGCTCCACTCTGGAAATAGACACAGAGG
AATTAACCTCTACGTCTTCAAGGACCTCACCTCCCAGAGGACGTCAGGAT
TCACTCAATGATTACTTAGATGCTATCGAACACAATGGCCACTCCAGGCC
AGGGACAGCGACCTGCTCTGAGAGGTCCATGGGTGCCTCTCCGAAACTGA
GGAGTAGTTTTCCCACTGACACAAGACTCAATGCCATGCTTCATATTGAT
TCAGATGAAGAAGATCACGAGTTCCAGCAAGACCTGGGTTACCCATCTTC
CTTGGAGGAGGAGGGAGGCCTGATCATGTTTAGCAGGGCATCCAGAGCTG
ATGATGGAAGCCTGACATCTCAGACAAAGCTGGAGGACAACCCTGTTGAG
AATGAGGAAGCCTCCACACACGAAGCTGCTTCCTTTGAGGATAAGCCAGA
AAATCTTCCAGAGCTTGCAGAGAGCTCCTTACCTGCAGGCCCAGCCCCAG
AGGAAGGTGAAGGCGGCCCAGAGCCTCAACCCAGTGCTGACCAGGGCAGT
GCTGAACTGTGTGGCTCTCAAGAGGTAGATCAGCCCACAAGTGGCGCAGA
CACAGGGACTTCCGATGCATCAGGAGGAAGCCGAAGAGCCGTCAGTGAGA
CCGAGTCTCTCGATCAGGGCTCTGAGCCTTCCCAGGTGTCCTCTGAAACA
GAACCCAGTGATCCTGCCAGGACAGAGAGTGTGAGCGAAGCCAGCACCAG
GCCTGAGGGAGAGAGTGACCTGGAATGCGCTGACAGCTCCTGCAATGAGA
GTGTGACCACGCAGCTGTCCTCTGTGGACACACGGTGCTCCTCTCTTGAG
AGCGCACGGTTTCCTGAAACCCCAGCCTTTTCTTCTCAGGAGGAGGAAGA
CGGAGCCTGTGCAGCAGAGCCCACCAGCAGTGGCCCTGCCGAAGGGTCGC
AGGAATCCGTGTGCACTGCTGGTTCTTTACCTGTGGTACAGGTGCCCAGT
GGGGAGGATGAAGGGCCAGGGGCAGAATCGGCCACTGTACCTGACCAGGA
GGAGCTGGGGGAGGTCTGGCAGCGGAGGGGGAGCCTGGAGGGAGCTGCAG
CTGCTGCCGAGAGCCCACCGCAAGAAGAAGGCAGTGCTGGGGAGGCCCAA
GGCACCTGTGAAGGGGCAACTGCCCAGGAGGAGGGCGCTACTGGAGGTTC
TCAAGCCAACGGCCACCAGCCACTGCGATCACTACCTTCAGTGCGCCAGG
ATGTTAGCCGGTACCAGAGGGTGGACGAGGCTCTCCCACCAAACTGGGAG
GCACGCATTGACAGCCACGGCAGGATCTTCTACGTGGATCACGTAAACAG
AACCACGACGTGGCAGCGACCGACAGCTCCCCCAGCCCCGCAGGTGCTGC
AGAGATCTAACTCCATACAGCAAATGGAGCAGCTGAACCGGCGATATCAG
AGTATCCGCAGAACCATGACCAATGAGAGGCCTGAGGAAAATACCAATGC
TATTGATGGGGCAGGGGAGGAAGCTGACTTTCACCAAGCCAGTGCAGATT
TCCGACGGGAGAACATCCTGCCTCACTCTACCTCCAGATCCAGGATCACG
TTGCTGTTACAGTCTCCCCCCGTGAAGTTCCTCATCAGCCCAGAGTTCTT
CACCGTGCTGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACA
CGTGTTTGAAGCACATGATCACCAAAGTCCGGAGGGACACCCACCACTTT
GAACGCTACCAGCATAACCGCGACCTTGTGGGATTCCTCAACATGTTCGC
GAACAAACAGCTAGAGCTGCCGCGGGGATGGGAAATGAAACATGATCACC
AGGGCAAGGCATTCTTTGTGGACCACAACTCCCGCACCACCACTTTCATT
GATCCCCGGCTCCCACTTCAGAGCAGTAGACCCACAAGTGCGCTGGTTCA
TCGGCAACACCTGACAAGGCAACGCAGCCACAGTGCGGGTGAGGTAGGAG
AAGATTCTCGACATGCAGGACCACCAGTTCTTCCCAGGCCATCCAGTACA
TTCAATACAGTCAGTAGGCCACAGTACCAGGACATGGTTCCAGTGGCTTA
CAATGACAAGATTGTTGCATTTCTACGTCAACCCAATATCTTTGAAATTC
TACAGGAGCGTCAACCTGATCTTACCAGGAATCACTCACTCAGGGAGAAG
ATCCAATTTATCCGAACTGAAGGGACTCCAGGATTGGTGCGCCTTTCAAG
CGATGCAGACCTTGTTATGTTGCTGAGCTTATTTGAAGAAGAGATAATGT
CATATGTGCCTCCACATGCCTTACTCCACCCCAGCTACTGTCAGTCCCCA
CGTGGCTCTCCCGTGTCATCTCCTCAGAACTCGCCAGGTACCCAGCGTGC
CAATGCCCGGGCTCCAGCCCCTTACAAGCGGGATTTCGAAGCCAAACTGA
GGAACTTTTACAGGAAGTTAGAGACTAAAGGATATGGACAAGGCCCAGGG
AAGTTAAAGTTAATTATCCGAAGAGATCACTTACTAGAAGATGCTTTTAA
TCAGATTATGGGCTACTCCAGAAAAGACCTGCAGAGAAATAAGCTATATG
TCACCTTCGTTGGGGAGGAAGGGCTGGATTACAGTGGGCCTTCTAGAGAG
TTTTTCTTCCTGGTATCCAGAGAACTCTTTAACCCATATTATGGCTTATT
TGAATATTCAGCCAATGACACATACACAGTACAAATAAGTCCTATGTCTG
CTTTTGTAGACAATCACCATGAATGGTTCCGATTCAGTGGTAGGATCCTT
GGTCTTGCACTAATACACCAGTATTTGTTGGATGCCTTCTTCACACGGCC
CTTTTATAAGGCTCTTCTCAGAATTCTATGTGACCTGAGTGACCTAGAAT
ACCTTGATGAAGAGTTCCATCAGAGCCTGCAGTGGATGAAAGACAATGAT
ATCCATGACATCCTAGACCTCACGTTCACTGTGAACGAAGAAGTATTTGG
GCAGATAACTGAACGAGAATTAAAGCCAGGGGGTGCCAATATCCCAGTTA
CAGAGAAGAACAAGAAGGAGTACATCGAGAGGATGGTGAAGTGGAGGATT
GAGAGGGGTGTTGTACAGCAAACAGAGAGCTTAGTGCGTGGCTTCTATGA
GGTGGTGGATGCCAGGCTGGTATCTGTTTTTGATGCAAGAGAACTGGAAT
TGGTCATCGCAGGCACAGCTGAAATAGACCTAAGTGATTGGAGAAACAAC
ACAGAATATAGAGGAGGATACCATGACAATCATATTGTAATTCGGTGGTT
CTGGGCTGCAGTGGAAAGATTCAACAATGAACAACGACTAAGGTTGTTAC
AGTTTGTTACAGGCACATCCAGCATTCCCTATGAAGGATTTGCTTCACTC
CGAGGGAGTAACGGCCCAAGAAGATTCTGTGTGGAGAAATGGGGGAAAAT
CACTGCTCTTCCCAGAGCGCATACATGTTTTAACCGTCTGGATCTGCCTC
CCTACCCATCCTTTTCCATGCTTTATGAAAAACTGTTGACAGCAGTTGAA
GAAACCAGTACTTTTGGACTTGAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTIDAAAVDMASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQSSMPENMTLQ
RANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYH
GISGALRATTPCITVKNPAVMMGAEGMEGGASGNLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIW
HREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGKLTIPVQRLLERQAIGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSVHEDASPEAVGTILGVNSV
NGDLGSPSDDEDMPGSHHDSQVCSNGPVSEDSAADGTPKHSFRTSSTLEIDTEELTSTSSRTSPPRGRQDSLNDYLDAIEHNGHSRPGTATCSERSMGAS
PKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMFSRASRADDGSLTSQTKLEDNPVENEEASTHEAASFEDKPENLPELAESSLPAG
PAPEEGEGGPEPQPSADQGSAELCGSQEVDQPTSGADTGTSDASGGSRRAVSETESLDQGSEPSQVSSETEPSDPARTESVSEASTRPEGESDLECADSS
CNESVTTQLSSVDTRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQESVCTAGSLPVVQVPSGEDEGPGAESATVPDQEELGEVWQRRGSLE
GAAAAAESPPQEEGSAGEAQGTCEGATAQEEGATGGSQANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAP
QVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTNAIDGAGEEADFHQASADFRRENILPHSTSRSRITLLLQSPPVKFLISPEFFTVLHSNPSAYRMF
TNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAG
EVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEE
EIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRN
KLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLS
DLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDAR
ELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRL
DLPPYPSFSMLYEKLLTAVEETSTFGLE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU33081
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No