cDNA Clone LIMK1

• Plasmid: pFBDual6P-3 LIMK1 285-647
• Parent Plasmid: pFastbacDual 6P3
• Expressed: GST LIMK1 285-647
• Genbank: P53667
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACGGCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGCAT
CGACAGGTCTCCGGGCGCTGGCTCACTGGGCTCCCCGGCCTCCCAGCGCA
AGGACCTGGGTCGCTCTGAGTCCCTCCGCGTAGTCTGCCGGCCACACCGC
ATCTTCCGGCCGTCGGACCTCATCCACGGGGAGGTGCTGGGCAAGGGCTG
CTTCGGCCAGGCTATCAAGGTGACACACCGTGAGACAGGTGAGGTGATGG
TGATGAAGGAGCTGATCCGGTTCGACGAGGAGACCCAGAGGACGTTCCTC
AAGGAGGTGAAGGTCATGCGATGCCTGGAACACCCCAACGTGCTCAAGTT
CATCGGGGTGCTCTACAAGGACAAGAGGCTCAACTTCATCACTGAGTACA
TCAAGGGCGGCACGCTCCGGGGCATCATCAAGAGCATGGACAGCCAGTAC
CCATGGAGCCAGAGAGTGAGCTTTGCCAAGGACATCGCATCAGGGATGGC
CTACCTCCACTCCATGAACATCATCCACCGAGACCTCAACTCCCACAACT
GCCTGGTCCGCGAGAACAAGAATGTGGTGGTGGCTGACTTCGGGCTGGCG
CGTCTCATGGTGGACGAGAAGACTCAGCCTGAGGGCCTGCGGAGCCTCAA
GAAGCCAGACCGCAAGAAGCGCTACACCGTGGTGGGCAACCCCTACTGGA
TGGCACCTGAGATGATCAACGGCCGCAGCTATGATGAGAAGGTGGATGTG
TTCTCCTTTGGGATCGTCCTGTGCGAGATCATCGGGCGGGTGAACGCAGA
CCCTGACTACCTGCCCCGCACCATGGACTTTGGCCTCAACGTGCGAGGAT
TCCTGGACCGCTACTGCCCCCCAAACTGCCCCCCGAGCTTCTTCCCCATC
ACCGTGCGCTGTTGCGATCTGGACCCCGAGAAGAGGCCATCCTTTGTGAA
GCTGGAACACTGGCTGGAGACCCTCCGCATGCACCTGGCCGGCCACCTGC
CACTGGGCCCACAGCTGGAGCAGCTGGACAGAGGTTTCTGGGAGACCTAC
CGGCGCGGCGAGAGCGGACTGCCTGCCCACCCTGAGGTCCCCGACTGAGC
GGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDGSSARQKPVLRSC
SIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGK
GCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVL
KFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASG
MAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRS
LKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVN
ADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSF
VKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD
*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU35154
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression; ; Estimated MW of expressed protein : 68920 Daltons