cDNA Clone PAK3

• Plasmid: pGEX6P-1-PAK3
• Parent Plasmid: pGEX6P1
• Expressed: GST PAK3
• Genbank: BC117355.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCTGACGGTCTGGATAATGAAGAGAAACCCCCGGCTCCTCC
ACTGAGGATGAATAGTAACAACCGGGATTCTTCAGCACTCAACCACAGCT
CCAAACCACTTCCCATGGCCCCTGAAGAGAAGAATAAGAAAGCCAGGCTT
CGCTCTATCTTCCCAGGAGGAGGGGATAAAACCAATAAGAAGAAGGAGAA
AGAGCGCCCAGAGATCTCTCTTCCTTCAGACTTTGAGCATACGATTCATG
TGGGGTTTGATGCAGTCACCGGGGAATTCACTGGAATTCCAGAGCAATGG
GCACGATTACTCCAAACTTCCAACATAACAAAATTGGAACAGAAGAAGAA
CCCACAAGCTGTTCTAGATGTTCTCAAATTCTATGATTCCAAAGAAACAG
TCAACAACCAGAAATACATGAGCTTTACATCAGGAGATAAAAGTGCACAT
GGATACATAGCAGCCCATCCTTCGAGTACAAAAACAGCATCTGAGCCTCC
ATTGGCCCCTCCTGTGTCTGAAGAAGAAGATGAAGAGGAAGAAGAAGAAG
AAGATGAAAATGAGCCACCACCAGTTATCGCACCAAGACCAGAGCATACA
AAATCAATCTATACTCGTTCTGTGGTTGAATCCATTGCTTCACCAGCAGT
ACCAAATAAAGAGGTCACACCACCCTCTGCTGAAAATGCCAATTCCAGTA
CTTTGTACAGGAACACAGATCGGCAAAGAAAAAAATCCAAGATGACAGAT
GAGGAGATCTTAGAGAAGCTAAGAAGCATTGTGAGTGTTGGGGACCCAAA
GAAAAAATACACAAGATTTGAAAAAATTGGTCAAGGGGCATCAGGTACTG
TTTATACAGCACTAGACATTGCAACAGGACAAGAGGTGGCCATAAAGCAG
ATGAACCTTCAACAGCAACCCAAGAAGGAATTAATTATTAATGAAATTCT
GGTCATGAGGGAAAATAAGAACCCTAATATTGTTAATTATTTAGATAGCT
ACTTGGTGGGTGATGAACTATGGGTAGTCATGGAATACTTGGCTGGTGGC
TCTCTGACTGATGTGGTCACAGAGACCTGTATGGATGAAGGACAGATAGC
AGCTGTCTGCAGAGAGTGCCTGCAAGCTTTGGATTTCCTGCACTCAAACC
AGGTGATCCATAGAGATATAAAGAGTGACAATATTCTTCTCGGGATGGAT
GGCTCTGTTAAATTGACTGACTTTGGGTTCTGTGCCCAGATCACTCCTGA
GCAAAGTAAACGAAGCACTATGGTGGGAACCCCATATTGGATGGCACCTG
AGGTGGTGACTCGAAAAGCTTATGGTCCGAAAGTTGATATCTGGTCTCTT
GGAATTATGGCAATTGAAATGGTGGAAGGTGAACCCCCTTACCTTAATGA
AAATCCACTCAGGGCATTGTATCTGATAGCCACTAATGGAACTCCAGAGC
TCCAGAATCCTGAGAGACTGTCAGCTGTATTCCGTGACTTTTTAAATCGC
TGTCTTGAGATGGATGTGGATAGGCGAGGATCTGCCAAGGAGCTTTTGTA
GGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSDGLDNEEKPPAPPLRMN
SNNRDSSALNHSSKPLPMAPEEKNKKARLRSIFPGGGDKTNKKKEKERPE
ISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQAV
LDVLKFYDSKETVNNQKYMSFTSGDKSAHGYIAAHPSSTKTASEPPLAPP
VSEEEDEEEEEEEDENEPPPVIAPRPEHTKSIYTRSVVESIASPAVPNKE
VTPPSAENANSSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYT
RFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRE
NKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCR
ECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKR
STMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLR
ALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35151
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 84259 Daltons