cDNA Clone GLK

• Synonyms: MAP4K3
• Plasmid: pFBHtb GLK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His GLK
• Genbank: AAN75850.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGA
CTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGTCTACA
AGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAA
TTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGAT
GAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTATCTCA
GGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTA
CAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATA
TGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAA
AAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGT
CATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAAT
TGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAG
TTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGG
GCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTT
TGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTC
AGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCAC
TTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGA
AAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCTTTGG
CAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCAT
GATTTCGATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAAT
TCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGAGATAA
CCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCA
CATCATGAACTTGATCTGCAACTGGAATATGGACAAGGACACCAAGGTGG
TTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCTGTTGAAGAAGAAT
TGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGAT
GATGAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCC
ACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATGCATTCTACTGAGG
ATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAGCCCAGCA
AAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACA
CAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTG
AACGAGATGGCTCATTATGTCAACAACAGAATGAACATAGAGGCACAAAC
CTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCT
TCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTA
ATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATGGATAAACCCAGAT
ACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTTATACCCT
CAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAA
GGTGTACATGGTTGTATGTAATGAACAATTGCTTGCTATCAATATCTGGT
AAAGCTTCTCAGCTTTATTCCCATAATTTACCAGGGCTTTTTGATTATGC
AAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTG
ACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACC
AAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTTACACGGGCCATAA
ATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAATGGGTTG
AACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCA
TGTCCACTTAGAATGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCC
TTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTCAACCAAGTGGTTC
GATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCA
GATACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATAC
CATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTAAATCTCCAAGGAA
GATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGATTTCCAG
ATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACA
TGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTT
CAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGACAGGGTCGTGGTT
TTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACAT
CCTGGCGGGTCATGAAAACAGTTACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMNPGFDLSRRNPQEDFELIQRI
GSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPN
IVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQ
GLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFI
GTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRA
LFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPF
VTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNV
REEKTRSEITFGQVKFDPPLRKETEPHHELDLQLEYGQGHQGGYFLGANK
SLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIF
IPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGN
GMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVH
MGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHE
TSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLP
VAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQ
TSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVS
RGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDC
CIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSF
RSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENS
Y*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32839
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expresssion; ; Estimated MW of expressed proteins : 102324 Daltons