cDNA Clone GLK

• Synonyms: MAP4K3
• Plasmid: pFBDual6P-1 GLK
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST GLK
• Genbank: AAN75850.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGA
CTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGTCTACA
AGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAA
TTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGAT
GAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTATCTCA
GGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTA
CAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATA
TGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAA
AAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGT
CATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAAT
TGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAG
TTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGG
GCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTT
TGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTC
AGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCAC
TTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGA
AAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCTTTGG
CAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCAT
GATTTCGATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAAT
TCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGAGATAA
CCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCA
CATCATGAACTTGATCTGCAACTGGAATATGGACAAGGACACCAAGGTGG
TTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCTGTTGAAGAAGAAT
TGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGAT
GATGAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCC
ACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATGCATTCTACTGAGG
ATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAGCCCAGCA
AAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACA
CAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTG
AACGAGATGGCTCATTATGTCAACAACAGAATGAACATAGAGGCACAAAC
CTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCT
TCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTA
ATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATGGATAAACCCAGAT
ACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTTATACCCT
CAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAA
GGTGTACATGGTTGTATGTAATGAACAATTGCTTGCTATCAATATCTGGT
AAAGCTTCTCAGCTTTATTCCCATAATTTACCAGGGCTTTTTGATTATGC
AAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTG
ACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACC
AAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTTACACGGGCCATAA
ATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAATGGGTTG
AACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCA
TGTCCACTTAGAATGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCC
TTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTCAACCAAGTGGTTC
GATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCA
GATACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATAC
CATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTAAATCTCCAAGGAA
GATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGATTTCCAG
ATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACA
TGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTT
CAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGACAGGGTCGTGGTT
TTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACAT
CCTGGCGGGTCATGAAAACAGTTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNPGFDLSRRNPQEDFELI
QRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCK
HPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRE
TLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRK
SFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHP
MRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQ
HPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTS
RNVREEKTRSEITFGQVKFDPPLRKETEPHHELDLQLEYGQGHQGGYFLG
ANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPK
SIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVA
LGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTP
KVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNE
LHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQ
KLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCG
ALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCV
GVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVC
LDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQG
RSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGH
ENSY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32838
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expresssion; ; Estimated MW of expressed proteins : 125776 Daltons