cDNA Clone PHLPP1

• Plasmid: pEBG6P-PHLPP1 150-600
• Parent Plasmid: pEBG6P
• Expressed: GST PHLPP1 150-600
• Genbank: NP_919431
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGCTCACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAA
CCCTAGCCTTCCAGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCA
AGTTGAAGAGTCTTAACCTTTCCAATAATCATTTAGGGGACTTCCCACTG
GCAGTCTGCAGTATTCCAACCCTGGCAGAGCTGAACGTGTCCTGCAATGC
CCTGCGATCAGTCCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACAT
TTTTGTTGGATGGAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAAC
ATGAAGCAGCTTAGTTATCTGGGTCTTTCTTTCAATGAATTTACTGACAT
TCCCGAAGTATTGGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTG
GAAACTGTGTGGAGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCAC
ATTAAACATGTGGATCTAAGGTTGAACGTAATTAGGAAGCTGATAGCAGA
TGAAGTGGACTTTCTACAGCATGTTACTCAGCTTGACCTACGAGACAATA
AGCTTGGTGATCTAGATGCTATGATTTTCAACAACATTGAAGTTTTACAC
TGTGAAAGGAATCAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAA
AGCGCTCTATGCCTCTTCTAATGAACTTGTTCAACTTGATGTTTACCCAG
TTCCAAATTATCTGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAAT
GTGCCTGAGTGGGTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGG
CCATAATCAAATATGTGAACTTCCTGCCCGCTTATTTTGTAATAGCAGTC
TCCGGAAACTACTGGCAGGACACAACCAGTTGGCAAGGCTGCCTGAAAGG
CTAGAAAGAACCTCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCT
TGAGCTCCCACCTAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGA
ACGCCTCTGCGAACAAACTGGAAAGCCTTCCTCCAGCCACGCTTTCCGAA
GAGACAAACAGTATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCAC
AGACAAATGTGTGCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTC
ACATGGCCTATAACCGACTTCAGAGTTTTCCAGCAAGTAAAATGGCGAAA
CTGGAGGAACTTGAAGAAATTGATCTCAGTGGGAATAAGCTGAAAGCCAT
CCCAACAACGATCATGAATTGCAGGCGCATGCACACCGTGATTGCTCACT
CCAACTGCATCGAGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAG
TGTGTGGACCTGTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAALTHL
NLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPT
LAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYL
GLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLR
LNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLV
TLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCE
SRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVE
VLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQ
ELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEI
DLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32721
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 79242 Daltons