cDNA Clone PIK4Ca1

• Plasmid: pFBHtb PIK4Ca1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His PIK4Ca1
• Genbank: NP_002641
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTGGAGCAGAA
ATTTGGCCTGTTTTCTGCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCT
CGGAAGCAAGTCAACCCAAACCCTGTCCCCCCGAAGTGACCCCCCACTAC
ATCTGGATCGACTTCCTGGTGCAGCGGTTTGAGATCGCCAAGTACTGCAG
CTCTGACCAAGTGGAGATCTTCTCCAGCCTGCTGCAGCGCTCCATGTCCC
TGAACATCGGCGGGGCCAAGGGGAGCATGAACCGGCACGTGGCGGCCATC
GGGCCCCGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTGCATGCCGA
TGTGGTTCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAAGATCTACT
CCACTGCCTTTGACTACTTCAGCTGTCCCCCAAAGTTCCCTACTCAAGGA
GAGAAGCGGCTGCGTGAAGACATAAGCATCATGATTAAATTTTGGACCGC
CATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCCCAG
ATAATCAGGACACCCGGAGCAACCTGGACATAACTGTCGGCTCTCGGCAA
CAAGCCACCCAAGGCTGGATCAACACATACCCCCTGTCCAGCGGCATGTC
CACCATCTCCAAGAAATCAGGCATGTCTAAGAAAACCAACCGGGGCTCCC
AGCTGCACAAATACTACATGAAGCGCAGGACGCTGCTGCTGTCCCTGCTG
GCCACTGAGATCGAGCGTCTCATCACATGGTACAACCCGCTGTCAGCCCC
GGAACTGGAACTAGACCAGGCCGGAGAGAACAGCGTGGCCAACTGGAGAT
CTAAGTACATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTC
GCCTGGAGCATCTCTCCCTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAA
GAACACAGAAGCCATTGGGAACGAAGTGACCCGTCTCGTTCGGTTGGACC
CGGGAGCCGTTAGTGATGTGCCTGAAGCAATCAAGTTCCTGGTCACCTGG
CACACCATCGACGCCGATGCTCCAGAGCTCAGCCATGTGCTGTGCTGGGC
GCCCACGGACCCACCCACAGGCCTCTCCTACTTCTCCAGCATGTACCCGC
CGCACCCTCTCACGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTTCCCT
CCGGACGCCATCCTCTTCTACATCCCCCAGATTGTGCAGGCCCTCAGGTA
CGACAAGATGGGCTATGTGCGGGAGTATATTCTGTGGGCAGCGTCTAAAT
CCCAGCTTCTGGCACACCAGTTCATCTGGAACATGAAGACTAACATTTAT
CTAGATGAAGAGGGCCACCAGAAAGACCCTGACATCGGCGACCTCCTGGA
TCAGTTGGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGCGAAGGACT
TTTACCAGCGGGAGTTTGATTTCTTTAACAAGATCACCAACGTGTCGGCT
ATCATCAAGCCCTACCCTAAAGGCGACGAGAGAAAGAAGGCTTGTCTGTC
GGCCCTGTCTGAAGTGAAGGTGCAGCCGGGCTGCTACCTGCCCAGCAACC
CTGAGGCCATTGTGCTGGACATCGACTACAAGTCTGGGACCCCGATGCAG
AGTGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAGGTGAAGCGATGTGG
AGTTAGTGAACTTGAAAAAGAAGGTCTGCGGTGCCGCTCAGACTCCGAGG
ATGAGTGCAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCTGGCAGGCA
GCCATCTTCAAGGTGGGAGACGACTGCCGGCAGGACATGCTGGCCCTGCA
GATCATCGACCTCTTCAAGAACATCTTCCAGCTGGTCGGCCTGGACCTCT
TTGTTTTTCCCTACCGCGTGGTGGCCACTGCCCCTGGGTGCGGGGTGATC
GAGTGCATCCCCGACTGCACCTCCCGGGACCAGCTGGGCCGCCAGACAGA
CTTCGGCATGTACGACTACTTCACACGCCAGTACGGGGATGAGTCCACTC
TGGCCTTCCAGCAGGCCCGCTACAACTTCATCCGAAGCATGGCCGCCTAC
AGCCTCCTGCTGTTCCTGCTGCAGATCAAGGACAGACACAACGGCAACAT
TATGCTGGACAAGAAGGGTCATATCATCCACATCGACTTTGGCTTCATGT
TTGAAAGCTCGCCGGGCGGCAATCTCGGCTGGGAACCCGACATCAAGCTG
ACGGATGAGATGGTGATGATCATGGGGGGCAAGATGGAGGCCACACCCTT
CAAGTGGTTCATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCT
ACATGGACGCGGTCGTCTCCCTGGTCACTCTCATGTTGGACACGGGCCTG
CCCTGTTTTCGCGGCCAGACAATCAAGCTCTTGAAGCACAGGTTTAGCCC
CAACATGACTGAGCGCGAGGCTGCAAATTTCATCATGAAGGTCATCCAGA
GCTGCTTCCTCAGCAACAGGAGCCGGACCTACGACATGATCCAGTACTAT
CAGAATGACATCCCCTACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMREMAGAWHMTVEQKFGLFSAE
IKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIF
SSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATI
RNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKY
LTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSG
MSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQA
GENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGN
EVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTG
LSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVR
EYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEIT
GSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKV
QPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKE
GLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKN
IFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYF
TRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGH
IIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMC
VRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREA
ANFIMKVIQSCFLSNRSRTYDMIQYYQNDIPY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32654
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression; ; Estimated MW of expressed protein : 100351 Daltons