cDNA Clone p70S6K2

• Plasmid: pEBG6P-p70S6K2
• Parent Plasmid: pEBG6P
• Expressed: GST p70S6K2
• Genbank: AAI36565.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCGTGTTTGATTTGGATTTGGAGACGGAGGAAGGCAG
CGAGGGCGAGGGCGAGCCAGAGCTCAGCCCCGCGGACGCATGTCCCCTTG
CCGAGTTGAGGGCAGCTGGCCTAGAGCCTGTGGGACACTATGAAGAGGTG
GAGCTGACTGAGACCAGCGTGAACGTTGGCCCAGAGCGCATCGGGCCCCA
CTGCTTTGAGCTGCTGCGTGTGCTGGGCAAGGGGGGCTATGGCAAGGTGT
TCCAGGTGCGAAAGGTGCAAGGCACCAACTTGGGCAAAATATATGCCATG
AAAGTCCTAAGGAAGGCCAAAATTGTGCGCAATGCCAAGGACACAGCACA
CACACGGGCTGAGCGGAACATTCTAGAGTCAGTGAAGCACCCCTTTATTG
TGGAACTGGCCTATGCCTTCCAGACTGGTGGCAAACTCTACCTCATCCTT
GAGTGCCTCAGTGGTGGCGAGCTCTTCACGCATCTGGAGCGAGAGGGCAT
CTTCCTGGAAGATACGGCCTGCTTCTACCTGGCTGAGATCACGCTGGCCC
TGGGCCATCTCCACTCCCAGGGCATCATCTACCGGGACCTCAAGCCCGAG
AACATCATGCTCAGCAGCCAGGGCCACATCAAACTGACCGACTTTGGACT
CTGCAAGGAGTCTATCCATGAGGGCGCCGTCACTCACACCTTCTGCGGCA
CCATTGAGTACATGGCCCCTGAGATTCTGGTGCGCAGTGGCCACAACCGG
GCTGTGGACTGGTGGAGCCTGGGGGCCCTGATGTACGACATGCTCACTGG
ATCGCCGCCCTTCACCGCAGAGAACCGGAAGAAAACCATGGATAAGATCA
TCAGGGGCAAGCTGGCACTGCCCCCCTACCTCACCCCAGATGCCCGGGAC
CTTGTCAAAAAGTTTCTGAAACGGAATCCCAGCCAGCGGATTGGGGGTGG
CCCAGGGGATGCTGCTGATGTGCAGAGACATCCCTTTTTCCGGCACATGA
ATTGGGACGACCTTCTGGCCTGGCGTGTGGACCCCCCTTTCAGGCCCTGT
CTGCAGTCAGAGGAGGACGTGAGCCAGTTTGATACCCGCTTCACACGGCA
GACGCCGGTGGACAGTCCTGATGACACAGCCCTCAGCGAGAGTGCCAACC
AGGCCTTCCTGGGCTTCACATACGTGGCGCCGTCTGTCCTGGACAGCATC
AAGGAGGGCTTCTCCTTCCAGCCCAAGCTGCGCTCACCCAGGCGCCTCAA
CAGTAGCCCCCGGGCCCCCGTCAGCCCCCTCAAGTTCTCCCCTTTTGAGG
GGTTTCGGCCCAGCCCCAGCCTGCCGGAGCCCACGGAGCTACCTCTACCT
CCACTCCTGCCACCGCCGCCGCCCTCGACCACCGCCCCTCTCCCCATCCG
TCCCCCCTCAGGGACCAAGAAGTCCAAGAGGGGCCGTGGGCGTCCAGGGC
GCTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAAVFDLDLETEE
GSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIG
PHCFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDT
AHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLERE
GIFLEDTACFYLAEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDF
GLCKESIHEGAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDML
TGSPPFTAENRKKTMDKIIRGKLALPPYLTPDARDLVKKFLKRNPSQRIG
GGPGDAADVQRHPFFRHMNWDDLLAWRVDPPFRPCLQSEEDVSQFDTRFT
RQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSIKEGFSFQPKLRSPRR
LNSSPRAPVSPLKFSPFEGFRPSPSLPEPTELPLPPLLPPPPPSTTAPLP
IRPPSGTKKSKRGRGRPGR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32610
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 80870 Daltons