cDNA Clone IKK alpha

• Plasmid: pCMV-HA IKK alpha K44A
• Parent Plasmid: pCMV5 HA2
• Expressed: HA IKK alpha K44A
• Genbank: O15111
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCC
CTGGGAGATGCGGGAGCGGCTGGGCACCGGCGGCTTCGGGAACGTCTGTC
TGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTGCGTCTTGTCGC
CTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATCCAGAT
TATGAAGAAGTTGAACCATGCCAATGTTGTAAAGGCCTGTGATGTTCCTG
AAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAGCAATGGAATAC
TGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTG
TGGACTTAAAGAAAGCCAGATACTTTCTTTACTAAGTGATATAGGGTCTG
GGATTCGATATTTGCATGAAAACAAAATTATACATCGAGATCTAAAACCT
GAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAAATAAT
TGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCTT
TTGTGGGAACACTGCAGTATCTGGCCCCAGAGCTCTTTGAGAATAAGCCT
TACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATGGTATTTGAATG
TATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGC
ATGAGAAGATTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTGAAGAG
ATGTCAGGAGAAGTTCGGTTTAGTAGCCATTTACCTCAACCAAATAGCCT
TTGTAGTTTAATAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTGA
ATTGCGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAG
CCAAGATGTTTTGTATTAATGGATCACATTTTGAATTTGAAGATAGTACA
CATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCTGTTACCACCTG
ATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTGGAATA
AATACTGGTTCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTGGATCC
TCGGAAACCAGCCTCTCAATGTGTTCTAGATGGAGTTAGAGGCTGTGATA
GCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCCA
TTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAG
CAAAATACAGCTTCCAATTATACAGCTGCGTAAAGTGTGGGCTGAAGCAG
TGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGCTCTTTCAGGGA
CAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTAACAAA
AATGAAGAACACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTGG
AGTTTTTTCACAAAAGCATTCAGCTTGACTTGGAGAGATACAGCGAGCAG
ATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAAT
GGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATACCTGG
AGGATCAGATTATGTCTTTGCATGCTGAAATCATGGAGCTACAGAAGAGC
CCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTGGAACAGCGTGC
CATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTACA
GTGACAGCACAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAG
GACCGTGTGCTCAAGGAGCTGTTTGGTCATTTGAGCAAGTTGTTGGGCTG
TAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCTCAGTA
ATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGGAAAAGGCAG
AAAGAAATATGGCATCTCCTTAAAATTGCATGTACACAGAGTTCTGCCCG
GTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCCTCAGACATCAG
CATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTG
GTAACTCCTCAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTT
GAACTGCCTTGGCCATTTAAGCACTATTATTCATGAGGCAAATGAGGAAC
AGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAATGAGCG
GCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMERPPGLRPGAGGPWEMRERLGTGGFG
NVCLYQHRELDLKIAIASCRLELSTKNRERWCHEIQIMKKLNHANVVKAC
DVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSD
IGSGIRYLHENKIIHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSL
CTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQP
FTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLIVEPMENWLQ
LMLNCDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFL
LPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVR
GCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVW
AEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLK
AKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVI
GYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSD
HSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEV
ALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTP
QTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEA
NEEQGNSMMNLDWSWLTE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32598
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression; ; Estimated MW of expressed protein : 86876 Daltons