cDNA Clone p70S6K1

• Synonyms: RPS6KB1, p70 S6 Kinase
• Plasmid: pEBG6P-p70S6K1
• Parent Plasmid: pEBG6P
• Expressed: GST p70S6K1
• Genbank: NP_003152.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAGGCGACGAAGGAGGCGGGACGGCTTTTACCCAGCCCCGGA
CTTCCGAGACAGGGAAGCTGAGGACATGGCAGGAGTGTTTGACATAGACC
TGGACCAGCCAGAGGACGCGGGCTCTGAGGATGAGCTGGAGGAGGGGGGT
CAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGAACTTGG
CATGGAACATTGTGAGAAATTTGAAATCTCAGAAACTAGTGTGAACAGAG
GGCCAGAAAAAATCAGACCAGAATGTTTTGAGCTACTTCGGGTACTTGGT
AAAGGGGGCTATGGAAAGGTTTTTCAAGTACGAAAAGTAACAGGAGCAAA
TACTGGGAAAATATTTGCCATGAAGGTGCTTAAAAAGGCAATGATAGTAA
GAAATGCTAAAGATACAGCTCATACAAAAGCAGAACGGAATATTCTGGAG
GAAGTAAAGCATCCCTTCATCGTGGATTTAATTTATGCCTTTCAGACTGG
TGGAAAACTCTACCTCATCCTTGAGTATCTCAGTGGAGGAGAACTATTTA
TGCAGTTAGAAAGAGAGGGAATATTTATGGAAGACACTGCCTGCTTTTAC
TTGGCAGAAATCTCCATGGCTTTGGGGCATTTACATCAAAAGGGGATCAT
CTACAGAGACCTGAAGCCGGAGAATATCATGCTTAATCACCAAGGTCATG
TGAAACTAACAGACTTTGGACTATGCAAAGAATCTATTCATGATGGAACA
GTCACACACACGTTTTGTGGAACAATAGAATACATGGCCCCTGAAATCTT
GATGAGAAGTGGCCACAATCGTGCTGTGGATTGGTGGAGTTTGGGAGCAT
TAATGTATGACATGCTGACTGGAGCACCCCCATTCACTGGGGAGAATAGA
AAGAAAACAATTGACAAAATCCTCAAATGTAAACTCAATTTGCCTCCCTA
CCTCACACAAGAAGCCAGAGATCTGCTTAAAAAGCTGCTGAAAAGAAATG
CTGCTTCTCGTCTGGGAGCTGGTCCTGGGGACGCTGGAGAAGTTCAAGCT
CATCCATTCTTTAGACACATTAACTGGGAAGAACTTCTGGCTCGAAAGGT
GGAGCCCCCCTTTAAACCTCTGTTGCAATCTGAAGAGGATGTAAGTCAGT
TTGATTCCAAGTTTACACGTCAGACACCTGTCGACAGCCCAGATGACTCA
ACTCTCAGTGAAAGTGCCAATCAGGTCTTTCTGGGTTTTACATATGTGGC
TCCATCTGTACTTGAAAGTGTGAAAGAAAAGTTTTCCTTTGAACCAAAAA
TCCGATCACCTCGAAGATTTATTGGCAGCCCACGAACACCTGTCAGCCCA
GTCAAATTTTCTCCTGGGGATTTCTGGGGAAGAGGTGCTTCGGCCAGCAC
AGCAAATCCTCAGACACCTGTGGAATACCCAATGGAAACAAGTGGCATAG
AGCAGATGGATGTGACAATGAGTGGGGAAGCATCGGCACCACTTCCAATA
CGACAGCCAAACTCTGGGCCATACAAAAAACAAGCTTTTCCCATGATCTC
CAAACGGCCAGAGCACCTGCGTATGAATCTATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMRRRRRRDGFYPA
PDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYE
LGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTG
ANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQ
TGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKG
IIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPE
ILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLP
PYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLAR
KVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTY
VAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASA
STANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPM
ISKRPEHLRMNL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32591
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 86555 Daltons