cDNA Clone UBE2B

• Plasmid: pET156P-1 UBE2B
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBE2B
• Genbank: NP_003328
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTCGACCC
CGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCCA
CCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAA
TGCAGTTATATTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTA
AACTAGTAATAGAATTTTCTGAAGAATATCCAAATAAACCACCAACTGTT
AGGTTTTTATCCAAAATGTTTCATCCAAATGTGTATGCTGATGGTAGCAT
ATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATATGATGTATCTT
CTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAGT
CCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATA
TGAGAAAAGAGTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCATAAG
CGGCCAAGGGCGAATTCC

Amino Acid Sequence

MYHHHHHSSGLEVLFQGPLGSSTPARRRLMRDFKRLQEDPPVGVSGAPSE
NNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPN
VYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLY
QENKREYEKRVSAIVEQSWNDS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32555
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal 6HIS