cDNA Clone PIK3C2B

• Plasmid: pFBDual GST 6P-3 PIK3C2B 155-1383
• Parent Plasmid: pFastbacDual 6P3
• Expressed: GST PIK3c2B 155-1382
• Genbank: BC136645
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACTCCCCACCTCCTCTGCCTCCCCGAGCTTCTATCTGGGATACCCC
TCCCCTGCCTCCCAGAAAGGGGTCCCCCTCATCCTCCAAGATCTCCCAGC
CCAGTGACATCAACACTTTCTCTTTGGTCGAACAATTGCCAGGCAAACTG
CTAGAGCATCGGATCCTAGAAGAGGAAGAGGTGCTGGGAGGTGGGGGTCA
GGGGCGCCTACTGGGGTCTGTGGACTATGATGGTATCAATGATGCAATTA
CTAGGCTCAACTTGAAATCGACCTATGATGCGGAGATGTTGCGGGATGCC
ACCAGGGGCTGGAAGGAGGGCCGAGGGCCGCTGGACTTCAGCAAAGACAC
CTCTGGAAAACCCGTGGCCAGGAGCAAGACTATGCCCCCTCAGGTGCCCC
CCCGCACCTATGCCTCCCGCTATGGCAACCGAAAGAATGCGACGCCTGGC
AAGAACCGCCGGATTTCTGCAGCCCCGGTGGGCTCCCGGCCCCACACTGT
TGCCAATGGCCATGAGTTGTTTGAGGTCTCAGAAGAGAGAGATGAGGAGG
TTGCTGCATTTTGCCACATGCTGGATATCCTTCGATCTGGCTCTGACATC
CAAGACTACTTCCTCACTGGCTATGTCTGGAGTGCTGTCACCCCTAGCCC
AGAGCACCTCGGGGATGAGGTCAACCTGAAGGTGACTGTGTTGTGTGACA
GGCTTCAAGAGGCACTCACTTTCACCTGCAACTGTTCCTCCACTGTAGAC
TTGCTTATCTACCAGACCCTGTGCTACACCCATGATGACCTGAGGAATGT
GGACGTGGGTGACTTTGTGCTAAAGCCCTGCGGGCTGGAGGAGTTCCTGC
AGAACAAGCATGCCTTGGGCAGTCATGAGTACATCCAATACTGCCGCAAG
TTTGACATTGACATTCGGCTACAGCTGATGGAGCAGAAGGTTGTGCGCAG
TGACCTGGCCCGGACGGTGAATGATGACCAGAGCCCCTCCACCTTGAACT
ACCTCGTCCATCTCCAAGAGAGGCCTGTCAAGCAGACCATCAGCAGGCAG
GCCCTGAGTCTTCTGTTCGACACTTACCACAATGAGGTGGATGCCTTCCT
GCTGGCTGATGGAGACTTCCCACTGAAGGCTGACAGGGTGGTCCAGTCCG
TCAAGGCCATCTGCAACGCCCTGGCCGCCGTGGAAACCCCTGAGATCACC
AGTGCTCTCAACCAGCTGCCCCCCTGCCCCTCCCGCATGCAGCCTAAAAT
TCAGAAGGATCCCAGTGTCTTGGCTGTGAGGGAAAACCGAGAGAAGGTCG
TGGAAGCCCTGACCGCTGCCATCTTGGACCTGGTGGAGCTGTACTGCAAC
ACATTCAACGCAGACTTCCAGACGGCAGTGCCCGGGAGCCGCAAGCATGA
CCTGGTCCAGGAGGCCTGCCATTTCGCCAGGTCCCTGGCCTTCACTGTCT
ATGCCACCCACCGCATCCCCATCATCTGGGCTACCAGCTATGAAGATTTC
TACCTCTCCTGCTCCCTCAGCCATGGCGGCAAGGAGCTGTGCAGCCCCCT
GCAGACCCGAAGAGCTCACTTCTCCAAGTACCTCTTCCACCTCATCGTCT
GGGACCAGCAGATCTGCTTCCCAGTGCAGGTGAACCGGCTGCCTCGGGAG
ACACTGCTGTGTGCCACTCTCTATGCTCTGCCCATCCCCCCACCGGGGAG
CTCCTCAGAGGCCAATAAGCAGCGGCGGGTGCCTGAAGCCCTGGGCTGGG
TCACTACCCCACTCTTCAACTTCAGGCAGGTCCTGACCTGTGGCCGGAAG
CTTCTGGGTTTGTGGCCAGCAACACAGGAAAATCCCAGCGCCCGTTGGAG
TGCACCTAATTTCCACCAGCCAGACAGTGTCATCCTGCAGATTGACTTCC
CCACCTCGGCCTTTGACATCAAGTTCACCAGCCCCCCTGGAGACAAGTTC
AGCCCCCGCTATGAGTTTGGCAGCCTCCGGGAAGAAGACCAGCGCAAGCT
TAAAGACATCATGCAGAAAGAGTCCTTGTACTGGCTCACTGATGCTGACA
AGAAGCGCCTGTGGGAGAAGCGATATTACTGCCACTCGGAGGTGAGCTCG
CTCCCCCTGGTGCTCGCCAGCGCCCCCAGCTGGGAGTGGGCTTGCCTGCC
TGACATCTATGTTCTCCTGAAGCAGTGGACCCACATGAACCACCAGGATG
CCCTGGGGCTCCTGCATGCCACCTTCCCGGACCAGGAGGTGCGTCGTATG
GCTGTGCAGTGGATTGGCTCACTCTCAGATGCTGAGCTGCTAGACTACCT
GCCCCAGCTGGTACAGGCCCTGAAGTATGAATGCTACCTGGACAGCCCGT
TGGTGCGCTTCCTCCTGAAACGAGCTGTGTCTGACTTGAGAGTGACTCAC
TACTTCTTCTGGTTACTGAAGGACGGCCTCAAGGACTCTCAGTTCAGCAT
CCGCTACCAGTATCTGCTGGCAGCCTTACTGTGCTGCTGTGGCAAGGGGC
TGAGAGAAGAGTTTAACCGCCAGTGCTGGCTTGTCAATGCCCTGGCCAAA
CTGGCCCAGCAGGTCCGGGAGGCAGCCCCATCTGCAAGGCAGGGAATCCT
CCGCACGGGCCTGGAGGAGGTGAAGCAGTTCTTTGCCCTCAATGGCTCGT
GCCGCTTGCCACTCAGCCCCAGTCTGCTGGTTAAGGGAATTGTGCCCAGG
GACTGTTCCTACTTCAACTCCAATGCTGTCCCCCTCAAACTCTCCTTCCA
AAATGTGGATCCCCTGGGTGAGAACATCCGTGTCATCTTCAAGTGTGGGG
ACGACCTTCGCCAGGACATGCTAACGCTGCAGATGATTCGCATCATGAGC
AAGATCTGGGTCCAGGAGGGGCTGGACATGCGCATGGTCATCTTCCGCTG
CTTCTCCACCGGCCGGGGCAGAGGGATGGTGGAGATGATCCCTAATGCTG
AGACCCTGCGTAAGATCCAGGTGGAGCATGGGGTGACCGGCTCGTTCAAG
GACCGGCCCCTGGCAGACTGGCTGCAGAAACACAACCCTGGGGAGGACGA
GTATGAGAAGGCTGTGGAGAACTTTATCTACTCCTGCGCTGGCTGCTGCG
TGGCCACGTACGTCTTGGGCATCTGTGACCGACATAACGACAACATCATG
CTGAAGACCACTGGTCACATGTTCCACATTGATTTTGGCCGCTTCCTGGG
CCATGCCCAGATGTTTGGCAACATCAAGCGGGACCGTGCCCCCTTTGTCT
TCACCTCGGACATGGCGTATGTCATCAACGGGGGTGACAAGCCTTCCAGC
CGCTTCCATGATTTTGTTGACCTTTGCTGCCAAGCCTACAACCTCATTCG
CAAGCACACCCACCTCTTCCTCAACCTTCTGGGCCTGATGTTGTCCTGTG
GGATCCCTGAACTCTCAGACCTGGAGGACCTCAAGTATGTGTACGATGCC
CTGAGGCCTCAGGATACAGAGGCCAATGCCACTACCTACTTCACTAGGTT
GATTGAGTCCAGCCTGGGCAGTGTAGCCACAAAGCTCAATTTTTTCATCC
ATAATCTGGCTCAGATGAAGTTCACGGGCTCAGATGACCGGCTGACCCTC
TCCTTTGCCTCCCGAACACACACTCTCAAGAGCTCTGGCCGAATCAGTGA
TGTTTTCCTCTGCCGCCATGAGAAGATCTTCCACCCCAACAAATAAGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDSPPPLPPRASIWD
TPPLPPRKGSPSSSKISQPSDINTFSLVEQLPGKLLEHRILEEEEVLGGG
GQGRLLGSVDYDGINDAITRLNLKSTYDAEMLRDATRGWKEGRGPLDFSK
DTSGKPVARSKTMPPQVPPRTYASRYGNRKNATPGKNRRISAAPVGSRPH
TVANGHELFEVSEERDEEVAAFCHMLDILRSGSDIQDYFLTGYVWSAVTP
SPEHLGDEVNLKVTVLCDRLQEALTFTCNCSSTVDLLIYQTLCYTHDDLR
NVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDIDIRLQLMEQKVV
RSDLARTVNDDQSPSTLNYLVHLQERPVKQTISRQALSLLFDTYHNEVDA
FLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQP
KIQKDPSVLAVRENREKVVEALTAAILDLVELYCNTFNADFQTAVPGSRK
HDLVQEACHFARSLAFTVYATHRIPIIWATSYEDFYLSCSLSHGGKELCS
PLQTRRAHFSKYLFHLIVWDQQICFPVQVNRLPRETLLCATLYALPIPPP
GSSSEANKQRRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENPSAR
WSAPNFHQPDSVILQIDFPTSAFDIKFTSPPGDKFSPRYEFGSLREEDQR
KLKDIMQKESLYWLTDADKKRLWEKRYYCHSEVSSLPLVLASAPSWEWAC
LPDIYVLLKQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLD
YLPQLVQALKYECYLDSPLVRFLLKRAVSDLRVTHYFFWLLKDGLKDSQF
SIRYQYLLAALLCCCGKGLREEFNRQCWLVNALAKLAQQVREAAPSARQG
ILRTGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLS
FQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIF
RCFSTGRGRGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGE
DEYEKAVENFIYSCAGCCVATYVLGICDRHNDNIMLKTTGHMFHIDFGRF
LGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNL
IRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFT
RLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFASRTHTLKSSGRI
SDVFLCRHEKIFHPNK

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32550
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression; ; Estimated MW of expressed protein : 166742 Daltons