cDNA Clone PPP5C

• Plasmid: pCMV-HA-1 PPP5C D274A
• Parent Plasmid: pCMV5
• Expressed: HA PPP5C D274A
• Genbank: NM_006247.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCGATGGCGGAGGGCGAGAGGACTGAGTGTGCTGAGCCCCC
CCGGGACGAACCCCCGGCTGATGGAGCTCTGAAGCGGGCAGAGGAGCTCA
AGACTCAGGCCAATGACTACTTCAAAGCCAAGGACTACGAGAACGCCATC
AAGTTCTACAGCCAGGCCATCGAGCTGAACCCCAGCAATGCCATCTACTA
TGGCAACCGCAGCCTGGCCTACCTGCGCACTGAGTGCTATGGCTACGCGC
TGGGAGACGCCACGCGGGCCATTGAGCTGGACAAGAAGTACATCAAGGGT
TATTACCGCCGGGCTGCCAGCAACATGGCACTGGGCAAGTTCCGGGCCGC
GCTGCGAGACTACGAGACGGTGGTCAAGGTGAAGCCCCATGACAAGGATG
CCAAAATGAAATACCAGGAGTGCAACAAGATCGTGAAGCAGAAGGCCTTT
GAGCGGGCCATCGCGGGCGACGAGCACAAGCGCTCCGTGGTGGACTCGCT
GGACATCGAGAGCATGACCATTGAGGATGAGTACAGCGGACCCAAGCTTG
AAGACGGCAAAGTGACAATCAGTTTCATGAAGGAGCTCATGCAGTGGTAC
AAGGACCAGAAGAAACTGCACCGGAAATGTGCCTACCAGATTCTGGTACA
GGTCAAAGAGGTCCTCTCCAAGCTGAGCACGCTCGTGGAAACCACACTCA
AAGAGACAGAGAAGATTACAGTATGTGGGGACACCCATGGCCAGTTCTAT
GACCTCCTCAACATATTCGAGCTCAACGGTTTACCCTCGGAGACCAACCC
CTATATATTTAATGGTGACTTTGTGGCCCGAGGCTCCTTCTCTGTAGAAG
TGATCCTCACCCTTTTCGGCTTCAAGCTCCTGTACCCAGATCACTTTCAC
CTCCTTCGAGGCAACCACGAGACAGACAACATGAACCAGATCTACGGTTT
CGAGGGTGAGGTGAAGGCCAAGTACACAGCCCAGATGTACGAGCTCTTTA
GCGAGGTGTTCGAGTGGCTCCCGTTGGCCCAGTGCATCAACGGCAAAGTG
CTGATCATGCACGGAGGCCTGTTCAGTGAAGACGGTGTCACCCTGGATGA
CATCCGGAAAATTGAGCGGAATCGACAACCCCCAGATTCAGGGCCCATGT
GTGACCTGCTCTGGTCAGATCCACAGCCACAGAACGGGCGCTCGATCAGC
AAGCGGGGCGTGAGCTGTCAGTTTGGGCCTGACGTCACCAAGGCCTTCTT
GGAAGAGAACAACCTGGACTATATCATCCGCAGCCACGAAGTCAAGGCCG
AGGGCTACGAGGTGGCTCACGGAGGCCGCTGTGTCACCGTCTTCTCTGCC
CCCAACTACTGCGACCAGATGGGGAACAAAGCCTCCTACATCCACCTCCA
GGGCTCTGACCTACGGCCTCAGTTCCACCAGTTCACAGCAGTGCCTCATC
CCAACGTCAAGCCCATGGCCTATGCCAACACGCTGCTGCAGCTAGGAATG
ATGTGAGAATTC

Amino Acid Sequence

MYPYDVPDYAGSMAMAEGERTECAEPPRDEPPADGALKRAEELKTQANDY
FKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRA
IELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQE
CNKIVKQKAFERAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTI
SFMKELMQWYKDQKKLHRKCAYQILVQVKEVLSKLSTLVETTLKETEKIT
VCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVARGSFSVEVILTLFG
FKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWL
PLAQCINGKVLIMHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSD
PQPQNGRSISKRGVSCQFGPDVTKAFLEENNLDYIIRSHEVKAEGYEVAH
GGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMA
YANTLLQLGMM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32529
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression; ; Estimated MW of expressed protein : 58191 Daltons