cDNA Clone PCTK1

• Plasmid: pcDNA5-FRT/TO-FLAG PCTK1 S153A S155A
• Parent Plasmid: pcDNA5 FRT/TO FLAG2
• Expressed: FLAG PCTK1 S153A S155A
• Genbank: NM_006201
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGATCGGATGAAGAAGATCAAACGGCAGCTGGCAATGAC
ACTCCGAGGTGGCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGCAGA
TAGGCCTGGATGAGAGTGGTGGTGGTGGCGGCAGTGACCCTGGAGAGGCC
CCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAG
CTCTGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGGG
AGAGTGACCAGGCTTCAGCCACGTCCTCGGATGAGGTGCAGTCTCCAGTG
AGAGTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCACTGAGGACAT
CAACAAGCGCCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACC
TGGAGAAGCTGACCCTCAATAGCCCCATCTTTGACAAGCCCCTCAGCCGC
CGCCTCCGTCGTGTCGCCCTAGCTGAGATTGGCTTTGGGAAACTGGAGAC
CTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACA
AAGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCAAGGAGATCAGA
CTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCT
ACTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCC
ACACGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTG
AAGCAGTACCTGGATGACTGTGGGAACATCATCAACATGCACAACGTGAA
ACTGTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCGGCAGA
AGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGG
GGAGAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCC
AACAAAGACATACTCCAATGAGGTGGTGACACTGTGGTACCGGCCCCCTG
ACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGACATGTGGGGT
GTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGG
CTCCACGGTGGAGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACCC
CAACTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCAAGACA
TACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCG
ACTTGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTTTGAGG
GTCGAAATCGGATCTCCGCAGAGGATGCCATGAAACATCCATTCTTCCTC
AGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGC
ACTAAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGA
TGCCTGACTCAGGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAA
GCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMDRMKKIKRQLAMTLRGGRGIDKTNGAP
EQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLKMGS
DGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPE
GYLEKLTLNSPIFDKPLSRRLRRVALAEIGFGKLETYIKLDKLGEGTYAT
VYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHD
IIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCH
RQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYR
PPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRIL
GTPTEETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQ
FEGRNRISAEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLRS
SSMPDSGRPAFRVVDTEF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32413
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal FLAG for mammalian expression.