cDNA Clone UBE2H

• Plasmid: pET156P UBE2H
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBE2H
• Genbank: NP_003335.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGT
GGTCAAGCTCATCGAGAGTAAACATGAGGTTACGATCCTGGGAGGACTTA
ATGAATTTGTAGTGAAGTTTTATGGACCACAAGGAACACCATATGAAGGC
GGAGTATGGAAAGTTAGAGTGGACCTACCTGATAAATACCCTTTCAAATC
TCCATCTATAGGATTCATGAATAAAATTTTCCATCCCAACATTGATGAAG
CGTCAGGAACTGTGTGTCTAGATGTAATTAATCAAACTTGGACAGCTCTC
TATGATCTTACCAATATATTTGAGTCCTTCCTGCCTCAGTTATTGGCCTA
TCCTAACCCCATAGATCCTCTCAATGGTGACGCTGCAGCCATGTACCTCC
ACCGACCAGAAGAATACAAGCAGAAAATTAAAGAGTACATCCAGAAATAC
GCCACGGAGGAGGCGCTGAAAGAACAGGAAGAGGGTACCGGGGACAGCTC
ATCGGAGAGCTCTATGTCTGACTTTTCCGAAGATGAGGCCCAGGATATGG
AGTTGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMSSPSPGKRRMDTDVVKLIESKHEVTI
LGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHP
NIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAA
AMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDE
AQDMEL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32149
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression; ; Estimated MW of expressed : 23099 Daltons