cDNA Clone Glycogen synthase; Glycogenin

• Plasmid: pFastbacDual 6P2 Glycogenin, Glycogen synthase
• Parent Plasmid: pFastbacDual 6P2
• Expressed: GST Glycogenin, Glycogen synthase
• Genbank: BC031096
• Tag: GST
• Species: Human

Sequence of Insert

DNA sequence of Glycogenin insert
CTCGAGGTCGACGGTATCGATAAGCTTGATATCCACTGTGGAATTCGCCC
TTGCGGATCCGCCACCATGGCAGATCAGGCCTTTGTGACACTAACCACAA
ACGATGCCTACGCCAAAGGTGCCCTGGTCCTGGGATCATCTCTGAAACAG
CACAGGACCACCAGGAGGCTGGTCGTGCTCGCCACCCCTCAGGTCTCAGA
CTCCATGAGAAAAGTTTTAGAGACAGTCTTTGATGAAGTCATCATGGTAG
ATGTCTTGGACAGTGGCGATTCTGCTCATCTAACCTTAATGAAGAGGCCA
GAGTTGGGTGTCACGCTGACAAAGCTCCACTGCTGGTCGCTTACACAGTA
TTCAAAATGTGTATTCATGGATGCAGATACTCTGGTCCTAGCAAATATTG
ATGATCTTTTTGACAGAGAAGAATTGTCAGCAGCACCAGACCCAGGGTGG
CCTGACTGCTTCAATTCCGGAGTCTTCGTTTATCAGCCTTCAGTTGAAAC
ATACAATCAGCTGTTGCATCTTGCTTCTGAGCAAGGTAGTTTTGATGGTG
GGGACCAAGGCATACTGAACACATTTTTTAGCAGCTGGGCAACAACAGAT
ATCAGAAAACACCTGCCGTTTATTTATAACCTAAGCAGCATCTCTATATA
CTCCTACCTCCCGGCATTTAAAGTGTTTGGTGCAAGTGCCAAAGTTGTGC
ATTTCCTGGGACGAGTCAAACCATGGAATTATACTTATGATCCCAAAACA
AAAAGTGTCAAAAGTGAGGCCCATGATCCCAACATGACTCATCCAGAGTT
TCTCATCCTGTGGTGGAACATCTTTACCACCAACGTTTTACCTCTGCTTC
AACAATTTGGCCTTGTCAAAGACACCTGCTCATATGTAAATGTGGAAGAT
GTCTCAGGAGCCATATCACATCTGTCCCTTGGGGAGATCCCAGCTATGGC
ACAGCCGTTTGTATCCTCGGAAGAACGGAAGGAACGATGGGAACAGGGCC
AGGCTGATTATATGGGAGCAGATTCCTTTGACAACATCAAGAGGAAACTT
GACACTTACCTCCAGTAGCTCGAGCCAAGGGCGAATTCCACATTGGTCGC
TGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGC

DNA Sequence of GYS1 insert
CTCGAGGAAACCATGCCTTTAAACCGCACTTTGTCCATGTCCTCACTGCC
AGGACTGGAGGACTGGGAGGATGAATTCGACCTGGAGAACGCAGTGCTCT
TCGAAGTGGCCTGGGAGGTGGCTAACAAGGTGGGTGGCATCTACACGGTG
CTGCAGACGAAGGCGAAGGTGACAGGGGACGAATGGGGCGACAACTACTT
CCTGGTGGGGCCGTACACGGAGCAGGGCGTGAGGACCCAGGTGGAACTGC
TGGAGGCCCCCACCCCGGCCCTGAAGAGGACACTGGATTCCATGAACAGC
AAGGGCTGCAAGGTGTATTTCGGGCGCTGGCTGATCGAGGGAGGCCCTCT
GGTGGTGCTCCTGGACGTGGGTGCCTCAGCTTGGGCCCTGGAGCGCTGGA
AGGGAGAGCTCTGGGATACCTGCAACATCGGAGTGCCGTGGTACGACCGC
GAGGCCAACGACGCTGTCCTCTTTGGCTTTCTGACCACCTGGTTCCTGGG
TGAGTTCCTGGCACAGAGTGAGGAGAAGCCACATGTGGTTGCTCACTTCC
ATGAGTGGTTGGCAGGCGTTGGACTCTGCCTGTGTCGTGCCCGGCGACTG
CCTGTAGCAACCATCTTCACCACCCATGCCACGCTGCTGGGGCGCTACCT
GTGTGCCGGTGCCGTGGACTTCTACAACAACCTGGAGAACTTCAACGTGG
ACAAGGAAGCAGGGGAGAGGCAGATCTACCACCGATACTGCATGGAAAGG
GCGGCAGCCCACTGCGCTCACGTCTTCACTACTGTGTCCCAGATCACCGC
CATCGAGGCACAGCACTTGCTCAAGAGGAAACCAGATATTGTGACCCCCA
ATGGGCTGAATGTGAAGAAGTTTTCTGCCATGCATGAGTTCCAGAACCTC
CATGCTCAGAGCAAGGCTCGAATCCAGGAGTTTGTGCGGGGCCATTTTTA
TGGGCATCTGGACTTCAACTTGGACAAGACCTTATACTTCTTTATCGCCG
GCCGCTATGAGTTCTCCAACAAGGGTGCTGACGTCTTCCTGGAGGCATTG
GCTCGGCTCAACTATCTGCTCAGAGTGAACGGCAGCGAGCAGACAGTGGT
TGCCTTCTTCATCATGCCAGCGCGGACCAACAATTTCAACGTGGAAACCC
TCAAAGGCCAAGCTGTGCGCAAACAGCTTTGGGACACGGCCAACACGGTG
AAGGAAAAGTTCGGGAGGAAGCTTTATGAATCCTTACTGGTTGGGAGCCT
TCCCGACATGAACAAGATGCTGGATAAGGAAGACTTCACTATGATGAAGA
GAGCCATCTTTGCAACGCAGCGGCAGTCTTTCCCCCCTGTGTGCACCCAC
AATATGCTGGATGACTCCTCAGACCCCATCCTGACCACCATCCGCCGAAT
CGGCCTCTTCAATAGCAGTGCCGACAGGGTGAAGGTGATTTTCCACCCGG
AGTTCCTCTCCTCCACAAGCCCCCTGCTCCCTGTGGACTATGAGGAGTTT
GTCCGTGGCTGTCACCTTGGAGTCTTCCCCTCCTACTATGAGCCTTGGGG
CTACACACCGGCTGAGTGCACGGTTATGGGAATCCCCAGTATCTCCACCA
ATCTCTCCGGCTTCGGCTGCTTCATGGAGGAACACATCGCAGACCCCTCA
GCTTACGGTATCTACATTCTTGACCGGCGGTTCCGCAGCCTGGATGATTC
CTGCTCGCAGCTCACCTCCTTCCTCTACAGTTTCTGTCAGCAGAGCCGGC
GGCAGCGTATCATCCAGCGGAACCGCACGGAGCGCCTCTCCGACCTTCTG
GACTGGAAATACCTAGGCCGGTACTATATGTCTGCGCGCCACATGGCGCT
GTCCAAGGCCTTTCCAGAGCACTTCACCTACGAGCCCAACGAGGCGGATG
CGGCCCAGGGGTACCGCTACCCACGGCCAGCCTCGGTGCCACCGTCGCCC
TCGCTGTCACGACACTCCAGCCCGCACCAGAGTGAGGACGAGGAGGATCC
CCGGAACGGGCCGCTGGAGGAAGACGGCGAGCGCTACGATGAGGACGAGG
AGGCCGCCAAGGACCGGCGCAACATCCGTGCACCAGAGTGGCCGCGCCGA
GCGTCCTGCACCTCCTCCACCAGCGGCAGCAAGCGCAACTCTGTGGACAC
GGCCACCTCCAGCTCACTCAGCACCCCGAGCGAGCCCCTCAGCCCCACCA
GCTCCCTGGGCGAGGAGCGTAACTAACTCGAG

Amino Acid Sequence

Protein Sequence of expressed construct
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVEVDGIDKLDIHCGI
RPCGSATMADQAFVTLTTNDAYAKGALVLGSSLKQHRTTRRLVVLATPQV
SDSMRKVLETVFDEVIMVDVLDSGDSAHLTLMKRPELGVTLTKLHCWSLT
QYSKCVFMDADTLVLANIDDLFDREELSAAPDPGWPDCFNSGVFVYQPSV
ETYNQLLHLASEQGSFDGGDQGILNTFFSSWATTDIRKHLPFIYNLSSIS
IYSYLPAFKVFGASAKVVHFLGRVKPWNYTYDPKTKSVKSEAHDPNMTHP
EFLILWWNIFTTNVLPLLQQFGLVKDTCSYVNVEDVSGAISHLSLGEIPA
MAQPFVSSEERKERWEQGQADYMGADSFDNIKRKLDTYLQ*

Protein sequence of glycogen synthase.
MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTK
AKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCK
VYFGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREAND
AVLFGFLTTWFLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVAT
IFTTHATLLGRYLCAGAVDFYNNLENFNVDKEAGERQIYHRYCMERAAAH
CAHVFTTVSQITAIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQS
KARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEFSNKGADVFLEALARLN
YLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKF
GRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLD
DSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGC
HLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGI
YILDRRFRSLDDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKY
LGRYYMSARHMALSKAFPEHFTYEPNEADAAQGYRYPRPASVPPSPSLSR
HSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCT
SSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32027
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Vector for baculovirus expression