cDNA Clone Pellino1

• Plasmid: pEBG6P-Pellino1 S76A
• Parent Plasmid: pEBG6P
• Expressed: GST Pellino1 S76A
• Genbank: Q96FA3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTTTCTCCTGATCAAGAAAATCATCCATTTAAAGCACCAGT
AAAATATGGTGAACTCATTGTCTTAGGGTATAATGGGTCTCTCCCAAATG
GCGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACCTAAG
GCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGC
TGCAAAGGCAATAAGCAACAAAGACCAGCATGCCATATCATATACTTTGT
CTCGGGCCCAGACTGTGGTGGTTGAATATACTCATGACAGCAACACAGAT
ATGTTTCAGATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAAC
TGACACGGTTCCTGGAAGTCAAAGTAATTCTGATACACAGTCAGTACAAA
GCACTATATCAAGATTTGCCTGCAGAATCATATGTGAACGGAATCCTCCC
TTTACAGCACGGATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTT
TCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATGGATG
GCTTGACCACTAATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACA
GAAGACTCCAAGCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGT
ATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGG
AAATTGAAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGT
GCAACATTGTTATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGT
GAAGCATTTAGAAGCTTTAAGACAGGAAATCAATGCAGCACGACCTCAGT
GCCCTGTAGGGTTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGAC
GTTGTAGATGAAAAACAACCATGGGTATATCTAAACTGCGGCCATGTACA
TGGCTATCATAACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTG
AATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGA
TGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAG
CCCGTGTGGGCATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCCCAGA
TCCCACTTCCTCATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGT
GCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGG
ACCTCTAGACTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMFSPDQENHPFKA
PVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTP
QAAKAISNKDQHAISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFV
VTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKN
IFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCG
NVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTP
TVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGH
VHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHA
FSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIF
QGPLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15993
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 73745 Daltons