cDNA Clone UBE2NL

• Plasmid: pGEX6P-1-UBE2NL
• Parent Plasmid: pGEX6P1
• Expressed: GST UBE2NL
• Genbank: NP_001013007
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCGAGCTGCCCCACAGGATCATCAAGGAAACCCAGCGTTTGCT
GGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCC
GTTATTTTCATGTGGTCATTGCTGGGGAATCAAAGGATTCCCCCTTTGAG
GGAGGGACTTTTAAACGTGAACTATTACTTGCAGAAGAATACCCAATGGC
AGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACA
AGTTGGAAAGAATAAGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCA
CTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAATGCTCC
CAATCCAGATGATCCATTAGCAAATGATGTAGTGGAGCAGTGGAAGACCA
ACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCC
ATGAATAGTATTTAAGAATTCCGAAGGGCGAATTCTGCAGATATCCATCA
CACTGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAELPHRIIKETQRLLAEPV
PGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKV
RFMTKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDD
PLANDVVEQWKTNEAQAIETARAWTRLYAMNSI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15990
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. N.b. numerous differences at DNA level but agrees with UBE2NL protein sequence ref.; ; Estimated MW of expressed protein : 44068 Daltons