cDNA Clone PCTK1

• Plasmid: pCMV-HA-2 PCTK1
• Parent Plasmid: pCMV5 HA2
• Expressed: HA PCTK1
• Genbank: NM_006201
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGAC
ACTCCGAGGTGGCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGCAGA
TAGGCCTGGATGAGAGTGGTGGTGGTGGCGGCAGTGACCCTGGAGAGGCC
CCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAG
CTCTGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGGG
AGAGTGACCAGGCTTCAGCCACGTCCTCGGATGAGGTGCAGTCTCCAGTG
AGAGTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCACTGAGGACAT
CAACAAGCGCCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACC
TGGAGAAGCTGACCCTCAATAGCCCCATCTTTGACAAGCCCCTCAGCCGC
CGCCTCCGTCGTGTCAGCCTATCTGAGATTGGCTTTGGGAAACTGGAGAC
CTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACA
AAGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCAAGGAGATCAGA
CTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCT
ACTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCC
ACACGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTG
AAGCAGTACCTGGATGACTGTGGGAACATCATCAACATGCACAACGTGAA
ACTGTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCGGCAGA
AGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGG
GGAGAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCC
AACAAAGACATACTCCAATGAGGTGGTGACACTGTGGTACCGGCCCCCTG
ACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGACATGTGGGGT
GTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGG
CTCCACGGTGGAGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACCC
CAACTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCAAGACA
TACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCG
ACTTGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTTTGAGG
GTCGAAATCGGATCTCCGCAGAGGATGCCATGAAACATCCATTCTTCCTC
AGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGC
ACTAAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGA
TGCCTGACTCAGGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAA
GCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMDRMKKIKRQLSMTLRGGRGIDKTNGA
PEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLKMG
SDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLP
EGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYA
TVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLH
DIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYC
HRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWY
RPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRI
LGTPTEETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLL
QFEGRNRISAEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLR
SSSMPDSGRPAFRVVDTEF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15988
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression.; ; Estimated MW of expressed protein : 58078 Daltons