cDNA Clone UBE2NL

• Plasmid: pMalP UBE2NL
• Parent Plasmid: pMAL6P
• Expressed: MBP UBE2NL
• Genbank: NP_001013007
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCGCCGAGCTGCCCCACAGGATCATCAAGGAAACCCAGCGTTTGCT
GGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCC
GTTATTTTCATGTGGTCATTGCTGGGGAATCAAAGGATTCCCCCTTTGAG
GGAGGGACTTTTAAACGTGAACTATTACTTGCAGAAGAATACCCAATGGC
AGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACA
AGTTGGAAAGAATAAGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCA
CTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAATGCTCC
CAATCCAGATGATCCATTAGCAAATGATGTAGTGGAGCAGTGGAAGACCA
ACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCC
ATGAATAGTATTTAAGAATTC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSAELPHRIIKETQRLLAEPVPGI
KAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKVRFM
TKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLA
NDVVEQWKTNEAQAIETARAWTRLYAMNSI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15977
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression. N.b. numerous differences at DNA level but agrees with UBE2NL protein sequence ref.; ; Estimated MW of expressed protein : 63982 Daltons