cDNA Clone UBE2NL

• Plasmid: pET156P UBE2NL
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBE2NL
• Genbank: NP_001013007
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCCGAGCTGCCCCACAGGATCATCAAGGAAACCCAGCGTTTGCT
GGCAGAACCAGTTCCTGGCATCAAAGCCGAACCAGATGAGAGCAACGCCC
GTTATTTTCATGTGGTCATTGCTGGGGAATCAAAGGATTCCCCCTTTGAG
GGAGGGACTTTTAAACGTGAACTATTACTTGCAGAAGAATACCCAATGGC
AGCCCCTAAAGTACGTTTCATGACCAAAATTTATCATCCTAATGTAGACA
AGTTGGAAAGAATAAGTTTAGATATTTTGAAAGATAAGTGGTCCCCAGCA
CTGCAGATCCGCACAGTTCTGCTATCGATCCAGGCCTTGTTAAATGCTCC
CAATCCAGATGATCCATTAGCAAATGATGTAGTGGAGCAGTGGAAGACCA
ACGAAGCCCAAGCCATAGAAACAGCTAGAGCATGGACTAGGCTATATGCC
ATGAATAGTATTTAAGAATTC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSAELPHRIIKETQRLLAEPVPGIKAEPD
ESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKVRFMTKIYH
PNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVE
QWKTNEAQAIETARAWTRLYAMNSI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15976
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression. N.b. numerous differences at DNA level but agrees with UBE2NL protein sequence ref.; ; Estimated MW of expressed protein : 19689 Daltons