cDNA Clone MKK1

• Synonyms: MAP2K1
• Plasmid: pGEX6P-MKK1 6His D208A
• Parent Plasmid: pGEX6P1
• Expressed: GST MKK1 6His D208A
• Genbank: NM_002755.3
• Tag: GST
• Species: Human

Sequence of Insert

ggatcccccaagaagaagccgacgcccatccagctgaacccggcccccga
cggctctgcagttaacgggaccagctctgcggagaccaacttggaggcct
tgcagaagaagctggaggagctagagcttgatgagcagcagcgaaagcgc
cttgaggcctttcttacccagaagcagaaggtgggagaactgaaggatga
cgactttgagaagatcagtgagctgggggctggcaatggcggtgtggtgt
tcaaggtctcccacaagccttctggcctggtcatggccagaaagctaatt
catctggagatcaaacccgcaatccggaaccagatcataagggagctgca
ggttctgcatgagtgcaactctccgtacatcgtgggcttctatggtgcgt
tctacagcgatggcgagatcagtatctgcatggagcacatggatggaggt
tctctggatcaagtcctgaagaaagctggaagaattcctgaacaaatttt
aggaaaagttagcattgctgtaataaaaggcctgacatatctgagggaga
agcacaagatcatgcacagagatgtcaagccctccaacatcctagtcaac
tcccgtggggagatcaagctctgtgcctttggggtcagcgggcagctcat
cgactccatggccaactccttcgtgggcacaaggtcctacatgtcgccag
aaagactccaggggactcattactctgtgcagtcagacatctggagcatg
ggactgtctctggtagagatggcggttgggaggtatcccatccctcctcc
agatgccaaggagctggagctgatgtttgggtgccaggtggaaggagatg
cggctgagaccccacccaggccaaggacccccgggaggccccttagctca
tacggaatggacagccgacctcccatggcaatttttgagttgttggatta
catagtcaacgagcctcctccaaaactgcccagtggagtgttcagtctgg
aatttcaagattttgtgaataaatgcttaataaaaaaccccgcagagaga
gcagatttgaagcaactcatggttcatgcttttatcaagagatctgatgc
tgaggaagtggattttgcaggttggctctgctccaccatcggccttaacc
agcccagcacaccaacccatgctgctggcgtcCATCATCACCATCACCAT
AAGGGCGAATTCCAGCACACTGGCGGCCGTTACTAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPKKKPTPIQLNPAPDGSAV
NGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEK
ISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHE
CNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVS
IAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCAFGVSGQLIDSMA
NSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKE
LELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNE
PPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVD
FAGWLCSTIGLNQPSTPTHAAGVHHHHHHKGEFQHTGGRY*

• Vector Type: Bacterial
• DU Number: DU15938
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Additional residue changes compared with reference: G328R and C376Y. Extra KGEFQHTGGRY residues after 6His tag.
• Low Temp: No