cDNA Clone PIAS4

• Plasmid: pGEX6P-2-PIAS3
• Parent Plasmid: pGEX6P2
• Expressed: GST PIAS4
• Genbank: AAH51252
• Tag: GST
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGGCGGAGCTGGGCGAGTTAAAGCACATGGTGATGAGTTT
CCGAGTGTCTGAGCTCCAGGTGCTCCTCGGCTTCGCTGGCAGGAACAAGA
GTGGGCGGAAACACGAGCTGCTGGCCAAGGCCCTGCACCTCCTCAAGTCT
AGCTGCGCCCCCAGCGTCCAGATGAAGATCAAAGAACTTTATCGCAGGCG
CTTTCCCCGGAAGACCCTGGGGCCTTCTGATCTCTCCTTGCTTTCTTTGC
CCCCTGGCACCTCTCCTGTAGGCTCCCCCGGCCCCCTTGCTCCCATCCCT
CCCACCCTTCTGACCCCTGGCACCTTGCTAGGCCCTAAGCGTGAGGTGGA
CATGCATCCTCCTCTGCCGCAGCCTGTGCACCCCGATGTCACCATGAAGC
CACTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGACCCACCACCCTT
GCGTCCACCTCCAGCCAGAGGTTCGAGGAAGCCCACTTCACCTTCGCGCT
CACTCCCCAGCAGCTGCAGCAGATTCTCACGTCCAGGGAGGTTCTGCCAG
GAGCCAAGTGTGATTACACCATACAAGTGCAGCTCAGATTCTGTCTCTGT
GAGACCAGCTGCCCTCAGGAGGACTATTTCCCCCCTAACCTCTTTGTTAA
GGTTAATGGGAAACTCTGCCCCCTGCCGGGTTACCTCCCTCCAACCAAGA
ATGGAGCTGAGCCCAAGAGGCCCAGCCGTCCGATCAACATCACACCCTTG
GCTCGACTCTCAGCCACTGTCCCCAACACCATCGTAGTTAATTGGTCATC
TGAGTTTGGACGGAATTACTCCTTGTCCGTGTACCTGGTGAGGCAATTGA
CTGCAGGGACCCTTCTACAAAAACTCAGAGCCAAGGGGATCCGGAATCCA
GACCATTCCCGGGCACTGATCAAGGAGAAACTGACTGCTGACCCCGACAG
TGAAGTGGCTACTACAAGTCTCCGGGTGTCACTCATGTGCCCGCTAGGGA
AGATGCGCCTGACTGTCCCGTGTCGTGCCCTCACCTGTGCCCATCTGCAG
AGTTTCGATGCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCGACATG
GACGTGTCCTGTGTGTGACAAGAAGGCTCCCTATGAGTCGCTGATTATTG
ATGGTTTATTCATGGAAATTCTTAATTCCTGTTCGGATTGTGATGAGATC
CAGTTCATGGAAGATGGATCCTGGTGTCCGATGAAACCCAAGAAGGAGGC
ATCAGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGATGGTCTCCAGTACA
GCGCAGTCCAGGAGGGAATTCAGCCAGAGAGTAAGAAGAGGGTCGAAGTC
ATTGACTTGACCATCGAAAGCTCATCAGATGAGGAGGATTTGCCCCCCAC
CAAGAAGCACTGCCCTGTCACCTCAGCGGCCATTCCAGCCCTTCCTGGAA
GCAAAGGAGCCCTGACCTCTGGTCACCAGCCATCCTCGGTGCTGCGGAGC
CCTGCAATGGGCACACTGGGCAGTGACTTCCTGTCTAGTCTCCCGCTACA
TGAGTACCCACCTGCCTTCCCACTGGGGGCTGACATCCAAGGTTTAGATT
TATTTTCTTTCCTTCAGACTGAGAGTCAGCACTACGGCCCTTCAGTTATC
ACTTCGCTAGATGAACAGGACACCCTTGGCCACTTCTTCCAGTACCGGGG
AACCCCTTCCCACTTCCTGGGCCCACTGGCCCCCACACTGGGGAGCTCTC
ACCGCAGCTCCACTCCAGCGCCCCCTCCTGGTCGTGTCAGCAGCATTGTG
GCTCCTGGGAGCTCCTTGAGGGAAGGGCATGGAGGACCCCTGCCTTCAGG
TCCCTCTTTGACTGGCTGTCGGTCAGACGTCATTTCCTTGGACTGAGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAMAELGELKH
MVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKE
LYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPGPLAPIPPTLLTPGTLLGP
KREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAH
FTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPP
NLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIV
VNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLT
ADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNE
KKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMK
PKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIESSSDEE
DLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLS
SLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHF
FQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLREGHGG
PLPSGPSLTGCRSDVISLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15930
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 96047 Daltons