cDNA Clone PCTK1

• Plasmid: pEBG6P-PCTK1 S12A S155A
• Parent Plasmid: pEBG6P
• Expressed: GST PCTK1 S12A S155A
• Genbank: NM_006201
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGATCGGATGAAGAAGATCAAACGGCAGCTGGCAATGAC
ACTCCGAGGTGGCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGCAGA
TAGGCCTGGATGAGAGTGGTGGTGGTGGCGGCAGTGACCCTGGAGAGGCC
CCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAG
CTCTGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGGG
AGAGTGACCAGGCTTCAGCCACGTCCTCGGATGAGGTGCAGTCTCCAGTG
AGAGTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCACTGAGGACAT
CAACAAGCGCCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACC
TGGAGAAGCTGACCCTCAATAGCCCCATCTTTGACAAGCCCCTCAGCCGC
CGCCTCCGTCGTGTCAGCCTAGCTGAGATTGGCTTTGGGAAACTGGAGAC
CTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACA
AAGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCAAGGAGATCAGA
CTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCT
ACTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCC
ACACGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTG
AAGCAGTACCTGGATGACTGTGGGAACATCATCAACATGCACAACGTGAA
ACTGTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCGGCAGA
AGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGG
GGAGAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCC
AACAAAGACATACTCCAATGAGGTGGTGACACTGTGGTACCGGCCCCCTG
ACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGACATGTGGGGT
GTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGG
CTCCACGGTGGAGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACCC
CAACTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCAAGACA
TACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCG
ACTTGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTTTGAGG
GTCGAAATCGGATCTCCGCAGAGGATGCCATGAAACATCCATTCTTCCTC
AGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGC
ACTAAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGA
TGCCTGACTCAGGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAA
GCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMDRM
KKIKRQLAMTLRGGRGIDKTNGAPEQIGLDESGGGGGSDPGEAPTRAAPG
ELRSARGPLSSAPEIVHEDLKMGSDGESDQASATSSDEVQSPVRVRMRNH
PPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSL
AEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGA
PCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDC
GNIINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLAD
FGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYE
MATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYR
AEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIH
KLPDTTSIFALKEIQLQKEASLRSSSMPDSGRPAFRVVDTEF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15823
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.