cDNA Clone USP45

• Synonyms: *
• Plasmid: pGEX6P-1-USP45
• Parent Plasmid: pGEX6P1
• Expressed: GST USP45
• Genbank: NP_001073950.1
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGCGGGTGAAAGATCCAACTAAAGCTTTACCTGAGAAAGCCAA
AAGAAGTAAAAGGCCTACTGTACCTCATGATGAAGACTCTTCAGATGATA
TTGCTGTAGGTTTAACTTGCCAACATGTAAGTCATGCTATCAGCGTGAAT
CATGTAAAGAGAGCAATAGCTGAGAATCTGTGGTCAGTTTGCTCAGAATG
TTTAAAAGAAAGAAGATTCTATGATGGGCAGCTAGTACTTACTTCTGATA
TTTGGTTGTGCCTCAAGTGTGGCTTCCAGGGATGTGGTAAAAACTCAGAA
AGCCAACATTCATTGAAGCACTTTAAGAGTTCCAGAACAGAGCCCCATTG
TATTATAATTAATCTGAGCACATGGATTATATGGTGTTATGAATGTGATG
AAAAATTATCAACGCATTGTAATAAGAAGGTTTTGGCTCAGATAGTTGAT
TTTCTCCAGAAACATGCTTCTAAAACACAAACAAGTGCATTTTCTAGAAT
CATGAAACTTTGTGAAGAAAAATGTGAAACAGATGAAATACAGAAGGGAG
GAAAATGCAGAAATTTATCTGTAAGAGGAATTACAAATTTAGGAAATACT
TGCTTTTTTAATGCAGTCATGCAGAACTTGGCACAGACTTATACTCTTAC
TGATCTGATGAATGAGATCAAAGAAAGTAGTACAAAACTCAAGATTTTTC
CTTCCTCAGACTCTCAGCTGGACCCATTGGTGGTGGAACTTTCAAGGCCT
GGACCACTGACTTCTGCCTTGTTCCTGTTTCTTCACAGCATGAAGGAGAC
TGAAAAAGGACCACTTTCTCCTAAAGTTCTTTTTAATCAGCTTTGTCAGA
AGGCACCTCGATTTAAAGATTTCCAGCAACAGGACAGTCAGGAGCTTCTT
CATTATCTTCTGGATGCAGTGAGGACAGAAGAAACAAAGCGAATACAAGC
TAGCATTCTAAAAGCATTTAACAACCCAACTACTAAAACTGCTGATGATG
AAACTAGAAAAAAAGTCAAAGCATATGGAAAAGAAGGTGTGAAAATGAAC
TTCATAGATCGGATCTTTATTGGTGAATTAACTAGCACGGTCATGTGTGA
AGAATGTGCAAATATCTCCACGGTGAAAGATCCATTCATTGATATTTCAC
TTCCTATAATAGAAGAAAGGGTTTCAAAACCTTTACTTTGGGGAAGAATG
AATAAATATAGAAGTTTACGGGAGACAGATCATGATCGATACAGTGGCAA
TGTTACTATAGAAAATATTCATCAACCTAGAGCTGCCAAGAAGCATTCTT
CATCTAAAGATAAGAGTCAACTAATTCATGACCGAAAATGTATTAGAAAA
TTGTCATCTGGAGAAACTGTCACATACCAGAAAAATGAAAACCTTGAAAT
GAATGGGGATTCTTTAATGTTTGCCAGCCTCATGAATTCTGAGTCACGTC
TGAATGAAAGCCCTACTGATGACAGTGAAAAAGAAGCCAGCCATTCTGAA
AGCAATGTTGATGCTGACAGTGAGCCTTCAGAATCTGAAAGTGCTTCAAA
GCAGACTGGGCTGTTCAGATCCAGTAGTGGATCCGGTGTGCAGCCAGATG
GACCCCTTTACCCTCTGTCAGCAGGTAAACTGCTGTACACCAAGGAGACT
GACAGTGGTGATAAGGAAATGGCAGAAGCTATTTCTGAACTTCGTTTGAG
CAGCACTGTAACTGGAGATCAAGATTTTGACAGAGAAAATCAGCCACTAA
ATATTTCAAATAATTTATGTTTTTTGGAGGGGAAGCATTTGAGGTCTTAT
AGTCCCCAAAATGCTTTTCAGACCCTTTCTCAGAGCTATATAACTACTTC
TAAAGAATGTTCAATTCAGTCCTGTCTCTACCAGTTTACATCTATGGAAT
TACTAATGGGGAATAATAAGCTTCTATGTGAGAATTGTACTAAAAACAAA
CAGAAGTACCAAGAAGAAACCAGTTTTGCAGAAAAGAAAGTAGAAGGAGT
TTATACTAATGCCAGGAAGCAATTGCTCATTTCTGCTGTTCCAGCTGTCC
TAATTCTCCACCTGAAAAGATTTCATCAGGCTGGCTTGAGTCTTCGTAAA
GTAAACAGACATGTAGATTTTCCACTTATGCTCGATTTAGCACCATTCTG
CTCTGCTACTTGTAAGAATGCAAGTGTGGGAGATAAAGTTCTCTACGGTC
TCTATGGCATAGTGGAACATAGTGGCTCGATGAGAGAAGGCCACTACACT
GCTTATGTGAAAGTGAGAACACCCTCCAGGAAATTATCGGAACATAACAC
TAAAAAGAAAAATGTGCCTGGTTTGAAAGCGGCTGATAATGAATCAGCAG
GCCAGTGGGTCCATGTTAGTGACACTTACTTACAGGTGGTTCCAGAATCA
AGAGCACTTAGTGCACAAGCCTACCTTCTTTTCTATGAAAGAGTATTATA
AGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMRVKDPTKALPEKAKRSKR
PTVPHDEDSSDDIAVGLTCQHVSHAISVNHVKRAIAENLWSVCSECLKER
RFYDGQLVLTSDIWLCLKCGFQGCGKNSESQHSLKHFKSSRTEPHCIIIN
LSTWIIWCYECDEKLSTHCNKKVLAQIVDFLQKHASKTQTSAFSRIMKLC
EEKCETDEIQKGGKCRNLSVRGITNLGNTCFFNAVMQNLAQTYTLTDLMN
EIKESSTKLKIFPSSDSQLDPLVVELSRPGPLTSALFLFLHSMKETEKGP
LSPKVLFNQLCQKAPRFKDFQQQDSQELLHYLLDAVRTEETKRIQASILK
AFNNPTTKTADDETRKKVKAYGKEGVKMNFIDRIFIGELTSTVMCEECAN
ISTVKDPFIDISLPIIEERVSKPLLWGRMNKYRSLRETDHDRYSGNVTIE
NIHQPRAAKKHSSSKDKSQLIHDRKCIRKLSSGETVTYQKNENLEMNGDS
LMFASLMNSESRLNESPTDDSEKEASHSESNVDADSEPSESESASKQTGL
FRSSSGSGVQPDGPLYPLSAGKLLYTKETDSGDKEMAEAISELRLSSTVT
GDQDFDRENQPLNISNNLCFLEGKHLRSYSPQNAFQTLSQSYITTSKECS
IQSCLYQFTSMELLMGNNKLLCENCTKNKQKYQEETSFAEKKVEGVYTNA
RKQLLISAVPAVLILHLKRFHQAGLSLRKVNRHVDFPLMLDLAPFCSATC
KNASVGDKVLYGLYGIVEHSGSMREGHYTAYVKVRTPSRKLSEHNTKKKN
VPGLKAADNESAGQWVHVSDTYLQVVPESRALSAQAYLLFYERVL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-USP45113.92 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU15681
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 118554 Daltons
• Low Temp: No