cDNA Clone BRAF

• Synonyms: RAF-b isoform
• Plasmid: pFB GST BRAF V600E new
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST Braf V600E
• Genbank: NP_004324.2
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACGCGGCGCTGAGCGGTGGCGGTGGTGGCGGCGCGGAGCCGGGCCA
GGCTCTGTTCAACGGGGACATGGAGCCCGAGGCCGGCGCCGGCGCCGGCG
CCGCGGCCTCTTCGGCTGCGGACCCTGCCATTCCGGAGGAGGTGTGGAAT
ATCAAACAAATGATTAAGTTGACACAGGAACATATAGAGGCCCTATTGGA
CAAATTTGGTGGGGAGCATAATCCACCATCAATATATCTGGAGGCCTATG
AAGAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTA
TTGGAATCTCTGGGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATC
AATGGATACCGTTACATCTTCTTCCTCTTCTAGCCTTTCAGTGCTACCTT
CATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCAACCCC
AAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG
GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGA
AAGCACTGATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGA
ATTCAGGATGGAGAGAAGAAACCAATTGGTTGGGACACTGATATTTCCTG
GCTTACTGGAGAAGAATTGCATGTGGAAGTGTTGGAGAATGTTCCACTTA
CAACACACAACTTTGTACGAAAAACGTTTTTCACCTTAGCATTTTGTGAC
TTTTGTCGAAAGCTGCTTTTCCAGGGTTTCCGCTGTCAAACATGTGGTTA
TAAATTTCACCAGCGTTGTAGTACAGAAGTTCCACTGATGTGTGTTAATT
ATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAACACCACCCA
ATACCACAGGAAGAGGCGTCCTTAGCAGAGACTGCCCTAACATCTGGATC
ATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTCACCA
GTCCGTCTCCTTCAAAATCCATTCCAATTCCACAGCCCTTCCGACCAGCA
GATGAAGATCATCGAAATCAATTTGGGCAACGAGACCGATCCTCATCAGC
TCCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGA
TTAGAGACCAAGGATTTCGTGGTGATGGAGGATCAACCACAGGTTTGTCT
GCTACCCCCCCTGCCTCATTACCTGGCTCACTAACTAACGTGAAAGCCTT
ACAGAAATCTCCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAG
AAGACAGGAATCGAATGAAAACACTTGGTAGACGGGACTCGAGTGATGAT
TGGGAGATTCCTGATGGGCAGATTACAGTGGGACAAAGAATTGGATCTGG
ATCATTTGGAACAGTCTACAAGGGAAAGTGGCATGGTGATGTGGCAGTGA
AAATGTTGAATGTGACAGCACCTACACCTCAGCAGTTACAAGCCTTCAAA
AATGAAGTAGGAGTACTCAGGAAAACACGACATGTGAATATCCTACTCTT
CATGGGCTATTCCACAAAGCCACAACTGGCTATTGTTACCCAGTGGTGTG
AGGGCTCCAGCTTGTATCACCATCTCCATATCATTGAGACCAAATTTGAG
ATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATGGATTA
CTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATAT
TTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACA
GAAAAATCTCGATGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATC
CATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCAT
ACAGCTTTCAGTCAGATGTATATGCATTTGGGATTGTTCTGTATGAATTG
ATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAGATAAT
TTTTATGGTGGGACGAGGATACCTGTCTCCAGATCTCAGTAAGGTACGGA
GTAACTGTCCAAAAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAG
AAAAGAGATGAGAGACCACTCTTTCCCCAAATTCTCGCCTCTATTGAGCT
GCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACCCTCCT
TGAATCGGGCTGGTTTCCAAACAGAGGATTTTAGTCTATATGCTTGTGCT
TCTCCAAAAACACCCATCCAGGCAGGGGGATATGGTGCGTTTCCTGTCCA
CTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDAALSGGGGGGAEP
GQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEAL
LDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSS
ASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNK
QRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDI
SWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTC
GYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTS
GSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSS
SAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVK
ALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIG
SGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNIL
LFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGM
DYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATEKSRWSGSHQFEQLS
GSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQ
IIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASI
ELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFP
VH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15485
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express N terminal GST fusion. ; ; Estimated MW of expressed protein : 111825 Daltons