cDNA Clone PPP5C

• Plasmid: pCMV5-FLAG PP5 D274A
• Parent Plasmid: pCMV5
• Expressed: FLAG PP5 D274A
• Genbank: NM_006247.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGGCGATGGCGGA
GGGCGAGAGGACTGAGTGTGCTGAGCCCCCCCGGGACGAACCCCCGGCTG
ATGGAGCTCTGAAGCGGGCAGAGGAGCTCAAGACTCAGGCCAATGACTAC
TTCAAAGCCAAGGACTACGAGAACGCCATCAAGTTCTACAGCCAGGCCAT
CGAGCTGAACCCCAGCAATGCCATCTACTATGGCAACCGCAGCCTGGCCT
ACCTGCGCACTGAGTGCTATGGCTACGCGCTGGGAGACGCCACGCGGGCC
ATTGAGCTGGACAAGAAGTACATCAAGGGTTATTACCGCCGGGCTGCCAG
CAACATGGCACTGGGCAAGTTCCGGGCCGCGCTGCGAGACTACGAGACGG
TGGTCAAGGTGAAGCCCCATGACAAGGATGCCAAAATGAAATACCAGGAG
TGCAACAAGATCGTGAAGCAGAAGGCCTTTGAGCGGGCCATCGCGGGCGA
CGAGCACAAGCGCTCCGTGGTGGACTCGCTGGACATCGAGAGCATGACCA
TTGAGGATGAGTACAGCGGACCCAAGCTTGAAGACGGCAAAGTGACAATC
AGTTTCATGAAGGAGCTCATGCAGTGGTACAAGGACCAGAAGAAACTGCA
CCGGAAATGTGCCTACCAGATTCTGGTACAGGTCAAAGAGGTCCTCTCCA
AGCTGAGCACGCTCGTGGAAACCACACTCAAAGAGACAGAGAAGATTACA
GTATGTGGGGACACCCATGGCCAGTTCTATGACCTCCTCAACATATTCGA
GCTCAACGGTTTACCCTCGGAGACCAACCCCTATATATTTAATGGTGACT
TTGTGGCCCGAGGCTCCTTCTCTGTAGAAGTGATCCTCACCCTTTTCGGC
TTCAAGCTCCTGTACCCAGATCACTTTCACCTCCTTCGAGGCAACCACGA
GACAGACAACATGAACCAGATCTACGGTTTCGAGGGTGAGGTGAAGGCCA
AGTACACAGCCCAGATGTACGAGCTCTTTAGCGAGGTGTTCGAGTGGCTC
CCGTTGGCCCAGTGCATCAACGGCAAAGTGCTGATCATGCACGGAGGCCT
GTTCAGTGAAGACGGTGTCACCCTGGATGACATCCGGAAAATTGAGCGGA
ATCGACAACCCCCAGATTCAGGGCCCATGTGTGACCTGCTCTGGTCAGAT
CCACAGCCACAGAACGGGCGCTCGATCAGCAAGCGGGGCGTGAGCTGTCA
GTTTGGGCCTGACGTCACCAAGGCCTTCTTGGAAGAGAACAACCTGGACT
ATATCATCCGCAGCCACGAAGTCAAGGCCGAGGGCTACGAGGTGGCTCAC
GGAGGCCGCTGTGTCACCGTCTTCTCTGCCCCCAACTACTGCGACCAGAT
GGGGAACAAAGCCTCCTACATCCACCTCCAGGGCTCTGACCTACGGCCTC
AGTTCCACCAGTTCACAGCAGTGCCTCATCCCAACGTCAAGCCCATGGCC
TATGCCAACACGCTGCTGCAGCTAGGAATGATGTGAGGATCC

Amino Acid Sequence

MDYKDDDDKAMAEGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAK
DYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELD
KKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKI
VKQKAFERAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTISFMK
ELMQWYKDQKKLHRKCAYQILVQVKEVLSKLSTLVETTLKETEKITVCGD
THGQFYDLLNIFELNGLPSETNPYIFNGDFVARGSFSVEVILTLFGFKLL
YPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQ
CINGKVLIMHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQ
NGRSISKRGVSCQFGPDVTKAFLEENNLDYIIRSHEVKAEGYEVAHGGRC
VTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYANT
LLQLGMM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15431
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 57827 Daltons