cDNA Clone PI3K alpha; p85

• Plasmid: pFB Dual 6His PI3K alpha, p85 tv2
• Parent Plasmid: pFastbacDual
• Expressed: 6His PI3K alpha,p85
• Genbank: AAH89038 and NP_001070963.1
• Tag: 6His
• Species: Mouse

Sequence of Insert

DNA sequence of PI3Kalpha
GGATCCATGCCTCCACGACCATCTTCGGGTGAACTGTGGGGCATCCACTT
GATGCCCCCACGAATCCTAGTGGAATGTTTACTCCCCAATGGAATGATAG
TGACTTTAGAATGCCTCCGTGAGGCCACACTCGTCACCATCAAACATGAA
CTGTTCAGAGAGGCCAGGAAATACCCTCTCCATCAGCTTCTGCAAGACGA
AACTTCTTACATTTTCGTAAGTGTCACCCAAGAAGCAGAAAGGGAAGAAT
TTTTTGATGAAACAAGACGACTTTGTGACCTTCGGCTTTTTCAACCCTTT
TTAAAAGTTATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCG
AGAAATTGGTTTTGTTATTGGCATGCCAGTGTGTGAATTTGATATGGTTA
AAGATCCAGAAGTCCAAGACTTTCGAAGGAACATTCTGAATGTTTGCAAA
GAAGCTGTGGACCTGCGGGATCTCAACTCGCCTCATAGCAGAGCAATGTA
TGTCTACCCTCCAAATGTCGAGTCTTCCCCAGAACTGCCAAAGCACATCT
ACAACAAGTTAGATAAAGGACAAATCATAGTGGTGATTTGGGTAATAGTC
TCTCCAAACAACGACAAGCAGAAGTACACTCTGAAGATCAATCATGACTG
TGTGCCAGAGCAAGTCATTGCTGAAGCCATCAGGAAAAAGACTCGGAGCA
TGTTGTTGTCCTCTGAGCAGCTGAAACTCTGTGTCTTAGAATATCAGGGC
AAGTATATTCTGAAAGTGTGTGGCTGTGACGAATACTTCCTGGAAAAGTA
CCCTCTGAGTCAGTACAAGTACATAAGAAGCTGTATAATGCTGGGGAGGA
TGCCCAACTTGATGCTGATGGCCAAAGAAAGCCTATACTCTCAGCTGCCG
ATTGATAGCTTCACCATGCCGTCATACTCCAGGCGCATCTCCACAGCCAC
ACCCTACATGAATGGAGAGACATCTACGAAATCCCTCTGGGTCATAAATA
GTGCGCTCAGAATAAAAATTCTTTGTGCAACCTATGTAAATGTAAATATT
CGAGACATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGA
ACCCTTATGTGACAATGTGAACACTCAAAGAGTACCTTGTTCCAATCCTA
GGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCCTCGT
GCTGCGCGCCTTTGCCTTTCAATCTGCTCTGTTAAAGGCCGAAAGGGTGC
TAAGGAGGAGCACTGTCCGTTGGCCTGGGGAAACATAAACTTGTTTGATT
ATACAGACACCCTAGTGTCCGGGAAAATGGCTTTGAATCTCTGGCCTGTA
CCGCATGGGTTAGAAGATCTGCTGAACCCTATTGGTGTTACTGGGTCAAA
TCCAAATAAAGAAACTCCATGCTTAGAGTTGGAGTTTGATTGGTTCAGCA
GTGTGGTGAAGTTTCCAGACATGTCTGTGATCGAAGAACATGCCAATTGG
TCCGTGTCCCGAGAAGCTGGATTCAGTTACTCCCATACAGGACTGAGTAA
CAGACTAGCCAGAGACAATGAGTTAAGAGAAAATGACAAGGAACAGCTCC
GAGCACTTTGCACCCGGGACCCACTATCTGAAATCACTGAACAAGAGAAA
GACTTCCTATGGAGCCACAGACACTACTGCGTAACTATTCCTGAAATCCT
ACCCAAATTGCTTCTGTCTGTCAAGTGGAATTCCAGAGACGAAGTGGCCC
AGATGTACTGCTTAGTAAAAGATTGGCCTCCAATCAAACCAGAGCAAGCC
ATGGAACTCCTGGACTGTAACTATCCAGATCCTATGGTTCGGAGTTTTGC
TGTTCGGTGCTTAGAAAAATATTTAACAGATGACAAACTTTCTCAGTACC
TCATTCAACTTGTACAGGTCTTAAAATATGAACAGTATTTGGATAACCTG
CTTGTGAGATTTTTACTCAAGAAAGCATTGACAAATCAAAGGATTGGCCA
TTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAATAAGACTGTCAGTC
AGAGGTTTGGCCTGCTATTGGAGTCCTACTGCCGTGCCTGTGGGATGTAT
CTGAAGCACCTGAACAGACAAGTAGAGGCCATGGAGAAGCTCATCAACCT
AACGGACATCCTTAAGCAGGAGAAGAAGGATGAGACACAAAAGGTACAGA
TGAAGTTTTTGGTTGAACAGATGAGACAGCCAGACTTCATGGATGCTTTG
CAGGGTTTTCTGTCCCCTCTGAATCCTGCTCACCAACTAGGAAACCTCAG
GCTTGAAGAGTGTCGAATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGA
ATTGGGAGAACCCAGACATCATGTCAGAGCTACTGTTTCAGAACAATGAG
ATCATCTTTAAAAATGGCGACGACTTACGGCAAGATATGTTAACCCTTCA
GATCATCCGAATCATGGAGAACATCTGGCAAAACCAAGGCCTTGACCTTC
GCATGCTACCTTATGGCTGTCTATCCATTGGGGACTGTGTGGGTCTCATC
GAGGTGGTGAGAAACTCTCACACCATCATGCAAATCCAGTGCAAAGGAGG
CCTGAAGGGGGCGCTGCAGTTCAACAGCCACACACTGCATCAATGGCTCA
AGGACAAGAACAAGGGCGAGATATATGATGCAGCCATTGACCTGTTCACT
CGGTCCTGCGCTGGGTACTGCGTGGCAACCTTTATCTTGGGAATTGGAGA
CCGGCACAACAGCAACATCATGGTGAAAGATGACGGACAGCTGTTTCATA
TAGATTTTGGGCACTTTTTGGATCACAAGAAGAAAAAATTTGGCTATAAG
CGGGAACGTGTGCCATTTGTGTTGACACAGGATTTCTTGATTGTGATTAG
TAAGGGAGCACAAGAGTACACCAAGACCAGAGAGTTTGAGAGGTTTCAGG
AGATGTGTTACAAGGCTTACCTAGCAATTCGGCAGCATGCCAATCTCTTC
ATCAACCTTTTTTCAATGATGCTTGGCTCTGGAATGCCAGAACTACAATC
TTTTGATGACATTGCATATATCCGAAAGACTCTAGCCTTGGACAAAACTG
AGCAAGAAGCTTTGGAATATTTCACAAAGCAAATGAATGATGCACATCAT
GGTGGATGGACGACAAAAATGGATTGGATCTTCCACACCATCAAGCAGCA
TGCTTTGAACTGAGCGGCCGC

DNA sequence of p85
CTCGAGGAAACCATGAGTGCAGAGGGCTACCAGTACAGAGCACTGTACGA
CTACAAGAAGGAGCGAGAGGAAGACATTGACCTACACCTGGGGGACATAC
TGACTGTGAATAAAGGCTCCTTAGTGGCACTTGGATTCAGTGATGGCCAG
GAAGCCCGGCCTGAAGATATTGGCTGGTTAAATGGCTACAATGAAACCAC
TGGGGAGAGGGGAGACTTTCCAGGAACTTACGTTGAATACATTGGAAGGA
AAAGAATTTCACCCCCTACTCCCAAGCCTCGGCCCCCTCGACCGCTTCCT
GTTGCTCCGGGTTCTTCAAAAACTGAAGCTGACACGGAGCAGCAAGCGTT
GCCCCTTCCTGACCTGGCCGAGCAGTTTGCCCCTCCTGATGTTGCCCCGC
CTCTCCTTATAAAGCTCCTGGAAGCCATTGAGAAGAAAGGACTGGAATGT
TCGACTCTATACAGAACACAAAGCTCCAGCAACCCTGCAGAATTACGGCA
GCTTCTTGATTGTGATGCCGCGTCAGTGGACTTGGAGATGATCGACGTAC
ACGTCTTAGCAGATGCTTTCAAACGCTATCTCGCCGACTTACCAAATCCT
GTCATTCCTGTAGCTGTTTACAATGAGATGATGTCTTTAGCCCAAGAACT
ACAGAGCCCTGAAGACTGCATCCAGCTGTTGAAGAAGCTCATTAGATTGC
CTAATATACCTCATCAGTGTTGGCTTACGCTTCAGTATTTGCTCAAGCAT
TTTTTCAAGCTCTCTCAAGCCTCCAGCAAAAACCTTTTGAATGCAAGAGT
CCTCTCTGAGATTTTCAGCCCCGTGCTTTTCAGATTTCCAGCCGCCAGCT
CTGATAATACTGAACACCTCATAAAAGCGATAGAGATTTTAATCTCAACG
GAATGGAATGAGAGACAGCCAGCACCAGCACTGCCCCCCAAACCACCCAA
GCCCACTACTGTAGCCAACAACAGCATGAACAACAATATGTCCTTGCAGG
ATGCTGAATGGTACTGGGGAGACATCTCAAGGGAAGAAGTGAATGAAAAA
CTCCGAGACACTGCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAA
AATGCACGGCGATTACACTCTTACACTAAGGAAAGGAGGAAATAACAAAT
TAATCAAAATCTTTCACCGTGATGGAAAATATGGCTTCTCTGATCCATTA
ACCTTCAACTCTGTGGTTGAGTTAATAAACCACTACCGGAATGAGTCTTT
AGCTCAGTACAACCCCAAGCTGGATGTGAAGTTGCTCTACCCAGTGTCCA
AATACCAGCAGGATCAAGTTGTCAAAGAAGATAATATTGAAGCTGTAGGG
AAAAAATTACATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATA
TGATAGATTATATGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGA
AAAGAACGGCTATCGAAGCATTTAATGAAACCATAAAAATATTTGAAGAA
CAATGCCAAACCCAGGAGCGGTACAGCAAAGAATACATAGAGAAGTTTAA
ACGCGAAGGCAACGAGAAAGAAATTCAAAGGATTATGCATAACCATGATA
AGCTGAAGTCGCGTATCAGTGAGATCATTGACAGTAGGAGGAGGTTGGAA
GAAGACTTGAAGAAGCAGGCAGCTGAGTACCGAGAGATCGACAAACGCAT
GAACAGTATTAAGCCGGACCTCATCCAGTTGAGAAAGACAAGAGACCAAT
ACTTGATGTGGCTGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAG
TGGCTGGGGAATGAAAATACCGAAGATCAATACTCCCTGGTAGAAGATGA
TGAGGATTTGCCCCACCATGACGAGAAGACGTGGAATGTCGGGAGCAGCA
ACCGAAACAAAGCGGAGAACCTATTGCGAGGGAAGCGAGACGGCACTTTC
CTTGTCCGGGAGAGCAGTAAGCAGGGCTGCTATGCCTGCTCCGTAGTGGT
AGACGGCGAAGTCAAGCATTGCGTCATTAACAAGACTGCCACCGGCTATG
GCTTTGCCGAGCCCTACAACCTGTACAGCTCCCTGAAGGAGCTGGTGCTA
CATTATCAACACACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCAC
ACTAGCATACCCAGTATATGCACAACAGAGGCGATGAGGTACC

Amino Acid Sequence

Expressed PI3K alpha Protein
MSYYHHHHHHDYDIPTTENLYFQGAMGSMPPRPSSGELWGIHLMPPRILV
ECLLPNGMIVTLECLREATLVTIKHELFREARKYPLHQLLQDETSYIFVS
VTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIG
MPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDLNSPHSRAMYVYPPNVE
SSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIA
EAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKY
IRSCIMLGRMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGET
STKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVN
TQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPL
AWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPC
LELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHTGLSNRLARDNE
LRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSV
KWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKY
LTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLK
SEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQVEAMEKLINLTDILKQE
KKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECRIM
SSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMEN
IWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHTIMQIQCKGGLKGALQF
NSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFILGIGDRHNSNIM
VKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYT
KTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYI
RKTLALDKTEQEALEYFTKQMNDAHHGGWTTKMDWIFHTIKQHALN*

Expressed p85 protein
MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARP
EDIGWLNGYNETTGERGDFPGTYVEYIGRKRISPPTPKPRPPRPLPVAPG
SSKTEADTEQQALPLPDLAEQFAPPDVAPPLLIKLLEAIEKKGLECSTLY
RTQSSSNPAELRQLLDCDAASVDLEMIDVHVLADAFKRYLADLPNPVIPV
AVYNEMMSLAQELQSPEDCIQLLKKLIRLPNIPHQCWLTLQYLLKHFFKL
SQASSKNLLNARVLSEIFSPVLFRFPAASSDNTEHLIKAIEILISTEWNE
RQPAPALPPKPPKPTTVANNSMNNNMSLQDAEWYWGDISREEVNEKLRDT
ADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNS
VVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLH
EYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQT
QERYSKEYIEKFKREGNEKEIQRIMHNHDKLKSRISEIIDSRRRLEEDLK
KQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGN
ENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRE
SSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQH
TSLVQHNDSLNVTLAYPVYAQQRR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15337
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression to express N ter 6His PI3Kalpha and untagged p85 tv2 from mouse.; ; Estimated MW of PI3Kalpha : 127781 Daltons; Estimated MW of p85 : 83514 Daltons