cDNA Clone PHLPP1

• Plasmid: pCMV-FLAG-2 PHLPP1
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG PHLPP
• Genbank: NP_919431
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACTCGGAGATTGGCTGCCTCATCCGCTTCTATGCAGG
AAAACCTCACAGCACGGGTAGCTCTGAACGGATTCAGCTCTCAGGAATGT
ATAATGTCCGTAAAGGCAAGATGCAGTTGCCAGTGAACCGATGGACAAGA
CGCCAAGTCATCCTATGTGGGACCTGCCTGATAGTATCATCTGTGAAAGA
CAGCTTGACCGGAAAGATGCATGTTCTGCCACTAATTGGTGGAAAAGTAG
AAGAAGTGAAAAAGCACCAACACTGTTTAGCATTTAGCTCCTCTGGACCC
CAAAGCCAGACTTACTACATTTGCTTTGATACTTTCACAGAATACTTAAG
GTGGCTGCGACAAGTCTCCAAGGTTGCATCCCAGCGCATTAGCTCAGTGG
ACCTCTCGTGTTGTAGCCTGGAACATCTGCCTGCCAACCTCTTCTACAGC
CAAGACCTCACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAACCC
TAGCCTTCCAGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCAAGT
TGAAGAGTCTTAACCTTTCCAATAATCATTTAGGGGACTTCCCTCTGGCA
GTCTGCAGTATTCCAACCCTGGCAGAGCTGAACGTGTCCTGCAATGCCCT
GCGATCAGTCCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACATTTT
TGTTGGATGGAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAACATG
AAGCAGCTTAGTTATCTGGGTCTTTCTTTCAATGAATTTACTGACATTCC
CGAAGTATTGGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTGGAA
ACTGTGTGGAGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCACATT
AAACATGTGGATCTAAGGTTGAACGTAATTAGGAAGCTGATAGCAGATGA
AGTGGACTTTCTACAGCATGTTACTCAGCTTGACCTACGAGACAATAAGC
TTGGTGATCTAGATGCTATGATTTTCAACAACATTGAAGTTTTACACTGT
GAAAGGAATCAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAAAGC
GCTCTATGCCTCTTCTAATGAACTTGTTCAACTTGATGTTTACCCAGTTC
CAAATTATCTGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAATGTG
CCTGAGTGGGTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGGCCA
TAATCAAATATGTGAACTTCCTGCCCGCTTATTTTGTAATAGCAGTCTCC
GGAAACTACTGGCAGGACACAACCAGTTGGCAAGGCTGCCTGAAAGGCTA
GAAAGAACCTCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCTTGA
GCTCCCACCTAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGAACG
CCTCTGCGAACAAACTGGAAAGCCTTCCTCCAGCCACGCTTTCCGAAGAG
ACAAACAGTATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCACAGA
CAAATGTGTGCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTCACA
TGGCCTATAACCGACTTCAGAGTTTTCCAGCAAGTAAAATGGCGAAACTG
GAGGAACTTGAAGAAATTGATCTCAGTGGGAATAAGCTGAAAGCCATCCC
AACAACGATCATGAATTGCAGGCGCATGCACACCGTGATTGCTCACTCCA
ACTGCATCGAGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAGTGT
GTGGACCTGAGCTGTAATGAGCTAAGTGAAGTCACATTACCAGAAAACCT
GCCTCCCAAACTGCAGGAGCTAGACCTGACTGGAAACCCGCGCCTTGTCC
TTGATCACAAAACCCTGGAACTACTGAATAATATCCGCTGTTTCAAGATT
GATCAGCCTTCTACAGGAGACGCTTCCGGAGCCCCAGCTGTATGGAGTCA
TGGTTACACTGAAGCTTCGGGGGTAAAAAACAAGTTGTGTGTCGCAGCCC
TGTCGGTGAATAACTTCTGTGACAACCGCGAAGCCCTGTATGGTGTGTTT
GACGGAGACCGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACTATGAG
TGACATTTTGGCTGAAGAGCTGCAAAAAACAAAAAACGAAGAAGAATACA
TGGTCAATACATTCATTGTCATGCAAAGGAAACTTGGAACTGCTGGGCAG
AAGCTTGGTGGTGCCGCTGTCCTTTGTCATATCAAGCATGACCCTGTGGA
TCCAGGAGGATCCTTCACCTTGACCTCTGCTAATGTGGGCAAGTGCCAAA
CAGTTCTCTGTCGAAATGGAAAGCCGCTGCCTCTGTCCAGATCTTACATC
ATGAGCTGTGAAGAAGAGCTGAAGAGGATTAAACAGCACAAGGCCATTAT
CACTGAGGATGGCAAGGTGAACGGAGTGACTGAGTCCACGCGCATCCTGG
GCTACACCTTCCTCCATCCCAGTGTGGTGCCTCGCCCCCACGTGCAGTCC
GTGCTCCTGACTCCCCAGGATGAGTTCTTCATCCTAGGCAGTAAGGGGTT
GTGGGACAGCCTGTCCGTCGAGGAGGCCGTGGAAGCCGTGCGCAACGTGC
CCGATGCCCTGGCTGCTGCCAAGAAGCTGTGTACCCTGGCCCAGAGCTAC
GGCTGCCACGACAGCATCAGCGCTGTGGTGGTGCAGCTCAGTGTCACTGA
GGACAGCTTCTGCTGCTGCGAGCTCAGCGCCGGTGGGGCTGTGCCACCAC
CCAGTCCTGGCATCTTTCCTCCCTCAGTGAACATGGTGATCAAGGATCGG
CCCTCAGATGGGCTGGGCGTGCCGTCCTCCAGCAGCGGCATGGCTTCCGA
GATTAGCAGTGAGCTCTCCACTTCTGAGATGAGCAGCGAGGTGGGGTCAA
CAGCCTCCGATGAGCCCCCGCCCGGAGCCCTAAGCGAGAACAGCCCTGCC
TACCCCAGTGAGCAGCGCTGCATGCTCCACCCCATCTGTCTGTCCAACTC
CTTCCAGCGCCAGCTATCCAGCGCCACGTTCTCTAGCGCCTTCTCCGACA
ACGGCCTTGACAGTGACGATGAGGAGCCCATCGAGGGCGTCTTCACCAAC
GGCAGCCGGGTGGAGGTGGAGGTGGACATCCACTGCAGCCGGGCCAAGGA
GAAGGAGAAACAGCAGCACCTGCTTCAGGTGCCAGCAGAGGCCAGTGATG
AGGGCATTGTCATCAGCGCCAACGAGGATGAGCCAGGTCTGCCCAGGAAG
GCAGACTTCTCTGCCGTTGGGACCATTGGGCGCCGGAGGGCCAATGGCTC
TGTTGCGCCCCAGGAAAGGAGCCACAATGTGATAGAGGTGGCTACAGACG
CACCTCTTCGAAAGCCTGGAGGCTATTTTGCTGCCCCGGCTCAGCCGGAT
CCTGATGATCAGTTTATCATACCCCCGGAGCTGGAAGAGGAGGTCAAAGA
AATCATGAAGCATCACCAGGAGCAACAGCAGCAGCAGCAGCCGCCACCAC
CCCCTCAGCTCCAGCCGCAGCTGCCGCGGCACTACCAGCTGGACCAGCTG
CCAGATTATTACGACACGCCACTATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMDSEIGCLIRFYAGKPHSTGSSERIQLS
GMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGG
KVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRIS
SVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRF
TKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQ
TFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCM
SGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRD
NKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVY
PVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNS
SLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRF
LNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKI
LHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIA
HSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPR
LVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCV
AALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEE
EYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGK
CQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTR
ILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVR
NVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAV
PPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEV
GSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAF
SDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEA
SDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVA
TDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQP
PPPPQLQPQLPRHYQLDQLPDYYDTPL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15246
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 135835 Daltons