cDNA Clone TBK1 NAP1

• Synonyms: Azi2
• Plasmid: pFB Dual GST TBK1 + NAP1
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST TBK1 + untagged NAP1
• Genbank: NP_037386 and NP_071906
• Tag: untagged
• Species: Human

Sequence of Insert

DNA sequence of TBK1 insert
GGATCCATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTT
AGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTG
GTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCA
GTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAA
AAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGACATA
AAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTA
GAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGT
TTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAG
TGCACCGTGATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGAT
GGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAATTAGA
AGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACC
CTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATAT
GGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGC
TACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAG
AAGTGATGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGA
GTACAGAAAGCAGAAAATGGACCAATTGACTGGAGTGGAGACATGCCTGT
TTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTTCTTG
CAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTT
TTTGCAGAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTC
GCTACAACAAATGACAGCTCATAAGATTTATATACATAGCTATAATACTG
CTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCA
AATCAAGAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAG
GCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTTGTAG
TAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCC
CTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGC
TAAGGCAATAACAGGGGTTGTGTGTTATGCCTGCAGAATTGCCAGTACCT
TACTGCTTTATCAGGAATTAATGCGAAAGGGGATACGATGGCTGATTGAA
TTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGT
GATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTAT
ATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATT
TCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAAT
AGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCAC
TGGCAGACGCATGGGCACATCAAGAAGGCACTCATCCGAAAGACAGAAAT
GTAGAAAAACTACAAGTCCTGTTAAATTGCATGACAGAGATTTACTATCA
GTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAA
TCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATG
ACGCACTTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAA
GTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTATTAT
CGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAA
GAATATACTAATGAGTTACAAGAAACTCTGCCTCAGAAAATGTTTACAGC
TTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCTAACA
CATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAA
GGGGTGGTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGG
CTCTTTAACCATGGATGGTGGCCTTCGCAACGTTGACTGTCTTTAGGCGG
CCGC

DNA insert of NAP1 CTCGAGGAAACCATGGATGCACTGGTAGAAGATGATATCTGTATTCTGAA
TCATGAAAAAGCCCATAAGAGAGATACAGTGACTCCAGTTTCAATATATT
CAGGAGATGAATCTGTTGCTTCCCATTTTGCTCTTGTCACTGCATATGAA
GACATCAAAAAACGACTTAAGGATTCAGAGAAAGAGAACTCTTTGTTAAA
GAAGAGAATAAGATTTTTGGAAGAAAAGCTAATAGCTCGATTTGAAGAAG
AAACAAGTTCCGTGGGACGAGAACAAGTAAATAAGGCCTATCATGCATAT
CGAGAGGTTTGCATTGATAGAGATAATTTGAAGAGCAAACTGGACAAAAT
GAATAAAGACAACTCTGAATCTTTGAAAGTATTGAATGAGCAGCTACAAT
CTAAAGAAGTAGAACTCCTCCAGCTGAGGACAGAGGTGGAAACTCAGCAG
GTGATGAGGAATTTAAATCCACCTTCATCAAACTGGGAGGTGGAAAAGTT
GAGCTGTGACCTGAAGATCCATGGTTTGGAACAAGAGCTGGAACTGATGA
GGAAAGAATGTAGCGATCTCAAAATAGAACTACAGAAAGCCAAACAAACG
GATCCATATCAGGAAGACAATCTGAAGAGCAGAGATCTCCAAAAACTAAG
CATTTCAAGTGATAATATGCAGCATGCATACTGGGAACTGAAGAGAGAAA
TGTCTAATTTACATCTGGTGACTCAAGTACAAGCTGAACTACTAAGAAAA
CTGAAAACCTCAACTGCAATCAAGAAAGCCTGTGCCCCTGTAGGATGCAG
TGAAGACCTTGGAAGAGACAGCACAAAACTGCACTTGATGAATTTTACTG
CAACATACACAAGACATCCCCCTCTCTTACCAAATGGCAAAGCTCTTTGT
CATACCACATCTTCCCCTTTACCAGGAGATGTAAAGGTTTTATCAGAGAA
AGCAATCCTCCAATCATGGACAGACAATGAGAGATCCATTCCTAATGATG
GTACATGCTTTCAGGAACACAGTTCTTATGGCAGAAATTCTCTGGAAGAC
AATTCCTGGGTATTTCCAAGTCCTCCTAAATCAAGTGAGACAGCATTTGG
GGAAACTAAAACTAAAACTTTGCCTTTACCCAACCTTCCACCACTGCATT
ACTTGGATCAACATAATCAGAACTGCCTTTATAAGAATTAAGCTAGC

Amino Acid Sequence

Protein Sequence of TBK1
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQSTSNHLWLLSDILGQGA
TANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVK
LFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDV
VGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQ
FVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSL
PFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSL
SRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQM
TAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQH
FPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAIT
GVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLD
FCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSL
QDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKD
KAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEW
IRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGI
KHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTM
DGGLRNVDCL*

Protein sequence for NAP1.
MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKK
RLKDSEKENSLLKKRIRFLEEKLIARFEEETSSVGREQVNKAYHAYREVC
IDRDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRN
LNPPSSNWEVEKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQ
EDNLKSRDLQKLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTS
TAIKKACAPVGCSEDLGRDSTKLHLMNFTATYTRHPPLLPNGKALCHTTS
SPLPGDVKVLSEKAILQSWTDNERSIPNDGTCFQEHSSYGRNSLEDNSWV
FPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQNCLYKN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15113
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminal GST-TBK1 and untagged NAP1 for baculovirus expression.; ; Estimated MW of TBK1 : 110463 Daltons; Estimated MW of NAP1 : 44933 Daltons