cDNA Clone GLK

• Synonyms: MAP4K3
• Plasmid: pEBG6P-GLK
• Parent Plasmid: pEBG6P
• Expressed: GST GLK
• Genbank: AAN75850.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGA
CTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGTCTACA
AGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAA
TTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGAT
GAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTATCTCA
GGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTA
CAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATA
TGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAA
AAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGT
CATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAAT
TGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAG
TTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGG
GCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTT
TGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTC
AGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCAC
TTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGA
AAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCTTTGG
CAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCAT
GATTTCGATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAAT
TCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGAGATAA
CCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCA
CATCATGAACTTGATCTGCAACTGGAATATGGACAAGGACACCAAGGTGG
TTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCTGTTGAAGAAGAAT
TGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGAT
GATGAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCC
ACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATGCATTCTACTGAGG
ATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAGCCCAGCA
AAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACA
CAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTG
AACGAGATGGCTCATTATGTCAACAACAGAATGAACATAGAGGCACAAAC
CTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCT
TCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTA
ATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATGGATAAACCCAGAT
ACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTTATACCCT
CAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAA
GGTGTACATGGTTGTATGTAATGAACAATTGCTTGCTATCAATATCTGGT
AAAGCTTCTCAGCTTTATTCCCATAATTTACCAGGGCTTTTTGATTATGC
AAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTG
ACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACC
AAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTTACACGGGCCATAA
ATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAATGGGTTG
AACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCA
TGTCCACTTAGAATGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCC
TTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTCAACCAAGTGGTTC
GATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCA
GATACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATAC
CATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTAAATCTCCAAGGAA
GATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGATTTCCAG
ATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACA
TGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTT
CAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGACAGGGTCGTGGTT
TTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACAT
CCTGGCGGGTCATGAAAACAGTTACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNPGFDLSRRNPQ
EDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEII
MMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQI
AYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITA
TIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPP
MFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPT
AEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPH
RIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELDLQLEYGQGHQ
GGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPP
LPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLP
PHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISN
GLPPTPKVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIY
TLNLNELHETSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFD
YARQMQKLPVAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTG
HKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQE
YPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLER
DTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFW
KHGMQGRSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNL
YILAGHENSY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15099
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 126369 Daltons