cDNA Clone TBK1

• Plasmid: pCMV-FLAG-1 TBK1 D157A
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG TBK1 D157A
• Genbank: NP_037386
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTT
AGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTG
GTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCA
GTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAA
AAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGACATA
AAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTA
GAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGT
TTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAG
TGCACCGTGATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGAT
GGACAGTCTGTGTACAAACTCACAGCTTTTGGTGCAGCTAGAGAATTAGA
AGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACC
CTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATAT
GGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGC
TACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAG
AAGTGATGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGA
GTACAGAAAGCAGAAAATGGACCAATTGACTGGAGTGGAGACATGCCTGT
TTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTTCTTG
CAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTT
TTTGCAGAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTC
GCTACAACAAATGACAGCTCATAAGATTTATATACATAGCTATAATACTG
CTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCA
AATCAAGAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAG
GCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTTGTAG
TAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCC
CTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGC
TAAGGCAATAACAGGGGTTGTGTGTTATGCCTGCAGAATTGCCAGTACCT
TACTGCTTTATCAGGAATTAATGCGAAAGGGGATACGATGGCTGATTGAA
TTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGT
GATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTAT
ATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATT
TCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAAT
AGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCAC
TGGCAGACGCATGGGCACATCAAGAAGGCACTCATCCGAAAGACAGAAAT
GTAGAAAAACTACAAGTCCTGTTAAATTGCATGACAGAGATTTACTATCA
GTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAA
TCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATG
ACGCACTTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAA
GTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTATTAT
CGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAA
GAATATACTAATGAGTTACAAGAAACTCTGCCTCAGAAAATGTTTACAGC
TTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCTAACA
CATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAA
GGGGTGGTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGG
CTCTTTAACCATGGATGGTGGCCTTCGCAACGTTGACTGTCTTTAGGCGG
CCGCGCAAGGGCGAATTGTGCAGATATCCATCACACTGGCGGCCGCTCGA
G

Amino Acid Sequence

MDYKDDDDKGSMQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKV
FNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFC
PCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPG
NIMRVIGEDGQSVYKLTAFGAARELEDDEQFVSLYGTEEYLHPDMYERAV
LRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIIT
GKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQ
EKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELV
YKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNT
IGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELM
RKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKIN
LEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQ
EGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQK
LYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFD
IEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLG
MKKLKEEMEGVVKELAENNHILERFGSLTMDGGLRNVDCL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 84866 Daltons