cDNA Clone TBK1; TANK

• Plasmid: pFB Dual GST TBK1 Tank1
• Parent Plasmid: pFastbacDual 6P1
• Expressed: 6His TBK1 untagged TANK
• Genbank: NP_037386 and AAH67779.1
• Tag: untagged
• Species: Human

Sequence of Insert

DNA Sequence of TBK1 insert :
GGATCCATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTT
AGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTG
GTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCA
GTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAA
AAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGACATA
AAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTA
GAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGT
TTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAG
TGCACCGTGATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGAT
GGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAATTAGA
AGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACC
CTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATAT
GGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGC
TACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAG
AAGTGATGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGA
GTACAGAAAGCAGAAAATGGACCAATTGACTGGAGTGGAGACATGCCTGT
TTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTTCTTG
CAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTT
TTTGCAGAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTC
GCTACAACAAATGACAGCTCATAAGATTTATATACATAGCTATAATACTG
CTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCA
AATCAAGAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAG
GCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTTGTAG
TAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCC
CTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGC
TAAGGCAATAACAGGGGTTGTGTGTTATGCCTGCAGAATTGCCAGTACCT
TACTGCTTTATCAGGAATTAATGCGAAAGGGGATACGATGGCTGATTGAA
TTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGT
GATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTAT
ATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATT
TCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAAT
AGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCAC
TGGCAGACGCATGGGCACATCAAGAAGGCACTCATCCGAAAGACAGAAAT
GTAGAAAAACTACAAGTCCTGTTAAATTGCATGACAGAGATTTACTATCA
GTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAA
TCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATG
ACGCACTTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAA
GTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTATTAT
CGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAA
GAATATACTAATGAGTTACAAGAAACTCTGCCTCAGAAAATGTTTACAGC
TTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCTAACA
CATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAA
GGGGTGGTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGG
CTCTTTAACCATGGATGGTGGCCTTCGCAACGTTGACTGTCTTTAGGGAT
CC

DNA Sequence of Tank1 :
GTCGACGAAACCATGGATGGATAAAAACATTGGCGAGCAACTCAATAAAG
CGTATGAAGCCTTCCGGCAGGCATGCATGGATAGAGATTCTGCAGTAAAA
GAATTACAGCAAAAGACTGAGAACTATGAGCAGAGAATACGTGAACAACA
GGAACAGCTGTCACTTCAACAGACTATTATTGACAAGCTAAAATCTCAGT
TACTTCTTGTGAATTCCACTCAAGATAACAATTATGGCTGTGTTCCTCTG
CTTGAAGACAGTGAAACAAGAAAGAATAATTTGACTCTTGATCAGCCACA
AGATAAAGTGATTTCAGGAATAGCAAGAGAAAAACTACCAAAGGTAAGAA
GACAAGAGGTTTCTTCTCCTAGAAAAGAAACTTCAGCAAGGAGTCTTGGC
AGTCCTTTGCTCCATGAAAGGGGTAATATAGAGAAGACTTTCTGGGATCT
GAAAGAAGAATTTCATAAAATATGCATGCTAGCAAAAGCACAGAAAGACC
ACTTAAGCAAACTTAATATACCAGACACTGCAACTGAAACACAGTGCTCT
GTGCCTATACAGTGTACGGATAAAACAGATAAACAAGAAGCGCTGTTTAA
GCCTCAGGCTAAAGATGATATAAATAGAGGTGCACCATCCATCACATCTG
TCACACCAAGAGGACTGTGCAGAGATGAGGAAGACACCTCTTTTGAATCA
CTTTCTAAATTCAATGTCAAGTTTCCACCTATGGACAATGACTCAACTTT
CTTACATAGCACTCCAGAGAGACCCGGCATCCTTAGTCCTGCCACGTCTG
AGGTAGTGTGCCAAGAGAAATTTAATATGGAGTTCAGAGACAACCCAGGG
AACTTTGTTAAAACAGAAGAAACTTTATTTGAAATTCAGGGAATTGACCC
CATAGCTTCAGCTATACAAAACCTTAAAACAACTGACAAAACAAAGCCCT
CAAATCTCGTAAACACTTGTATCAGGACAACTCTGGATAGAGCTGCGTGT
TTGCCACCTGGAGACCATAATGCATTATATGTAAATAGCTTCCCACTTCT
GGACCCATCTGATGCACCTTTTCCCTCACTCGATTCCCCGGGAAAAGCAA
TCCGAGGACCACAGCAGCCCATTTGGAAGCCCTTTCCTAATCAAGACAGT
GACTCGGTGGTACTAAGTGGCACAGACTCAGAACTGCATATACCTCGAGT
ATGTGAATTCTGTCAAGCAGTTTTCCCACCATCCATTACATCCAGGGGGG
ATT

Amino Acid Sequence

Protein sequence of TBK1 :
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQSTSNHLWLLSDILGQGA
TANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVK
LFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDV
VGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQ
FVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSL
PFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSL
SRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQM
TAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQH
FPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAIT
GVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLD
FCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSL
QDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKD
KAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEW
IRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGI
KHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTM
DGGLRNVDCL*

Protein sequence of Tank :
MDKNIGEQLNKAYEAFRQACMDRDSAVKELQQKTENYEQRIREQQEQLSL
QQTIIDKLKSQLLLVNSTQDNNYGCVPLLEDSETRKNNLTLDQPQDKVIS
GIAREKLPKVRRQEVSSPRKETSARSLGSPLLHERGNIEKTFWDLKEEFH
KICMLAKAQKDHLSKLNIPDTATETQCSVPIQCTDKTDKQEALFKPQAKD
DINRGAPSITSVTPRGLCRDEEDTSFESLSKFNVKFPPMDNDSTFLHSTP
ERPGILSPATSEVVCQEKFNMEFRDNPGNFVKTEETLFEIQGIDPIASAI
QNLKTTDKTKPSNLVNTCIRTTLDRAACLPPGDHNALYVNSFPLLDPSDA
PFPSLDSPGKAIRGPQQPIWKPFPNQDSDSVVLSGTDSELHIPRVCEFCQ
AVFPPSITSRGDFLRHLNSHFNGET*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU15003
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST TBK1 and untagged Tank for baculovirus expression.; ; Estimated MW of expressed protein : 47842 Daltons