cDNA Clone PI3K alpha; p85

• Plasmid: pFB Dual 6His PI3K alpha E545K, p85
• Parent Plasmid: pFastbacDual
• Expressed: 6His PI3K alpha E545K, p85
• Genbank: U79143 , NM_181523
• Tag: 6His
• Species: Human

Sequence of Insert

DNA sequence of insert 1 :
CTCGAGGAAACCATGCACCATCACCATCACCATCCTCCAAGACCATCATC
AGGTGAACTGTGGGGCATCCACTTGATGCCCCCAAGAATCCTAGTAGAAT
GTTTACTACCAAATGGAATGATAGTGACTTTAGAATGCCTCCGTGAGGCT
ACATTAATAACCATAAAGCATGAACTATTTAAAGAAGCAAGAAAATACCC
CCTCCATCAACTTCTTCAAGATGAATCTTCTTACATTTTCGTAAGTGTTA
CTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTGT
GACCTTCGGCTTTTTCAACCCTTTTTAAAAGTAATTGAACCAGTAGGCAA
CCGTGAAGAAAAGATCCTCAATCGAGAAATTGGTTTTGCTATCGGCATGC
CAGTGTGTGAATTTGATATGGTTAAAGATCCAGAAGTACAGGACTTCCGA
AGAAATATTCTGAACGTTTGTAAAGAAGCTGTGGATCTTAGGGACCTCAA
TTCACCTCATAGTAGAGCAATGTATGTCTATCCTCCAAATGTAGAATCTT
CACCAGAATTGCCAAAGCACATATATAATAAATTAGATAAAGGGCAAATA
ATAGTGGTGATCTGGGTAATAGTTTCTCCAAATAATGACAAGCAGAAGTA
TACTCTGAAAATCAACCATGACTGTGTACCAGAACAAGTAATTGCTGAAG
CAATCAGGAAAAAAACTCGAAGTATGTTGCTATCCTCTGAACAACTAAAA
CTCTGTGTTTTAGAATATCAGGGCAAGTATATTTTAAAAGTGTGTGGATG
TGATGAATACTTCCTAGAAAAATATCCTCTGAGTCAGTATAAGTATATAA
GAAGCTGTATAATGCTTGGGAGGATGCCCAATTTGATGTTGATGGCTAAA
GAAAGCCTTTATTCTCAACTGCCAATGGACTGTTTTACAATGCCATCTTA
TTCCAGACGCATTTCCACAGCTACACCATATATGAATGGAGAAACATCTA
CAAAATCCCTTTGGGTTATAAATAGTGCACTCAGAATAAAAATTCTTTGT
GCAACCTACGTGAATGTAAATATTCGAGACATTGATAAGATCTATGTTCG
AACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAACACTC
AAAGAGTACCTTGTTCCAATCCCAGGTGGAATGAATGGCTGAATTATGAT
ATATACATTCCTGATCTTCCTCGTGCTGCTCGACTTTGCCTTTCCATTTG
CTCTGTTAAAGGCCGAAAGGGTGCTAAAGAGGAACACTGTCCATTGGCAT
GGGGAAATATAAACTTGTTTGATTACACAGACACTCTAGTATCTGGAAAA
ATGGCTTTGAATCTTTGGCCAGTACCTCATGGATTAGAAGATTTGCTGAA
CCCTATTGGTGTTACTGGATCAAATCCAAATAAAGAAACTCCATGCTTAG
AGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTCCCAGATATGTCA
GTGATTGAAGAGCATGCCAATTGGTCTGTATCCCGAGAAGCAGGATTTAG
CTATTCCCACGCAGGACTGAGTAACAGACTAGCTAGAGACAATGAATTAA
GGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTC
TCTGAAATCACTAAGCAGGAGAAAGATTTTCTATGGAGTCACAGACACTA
TTGTGTAACTATCCCCGAAATTCTACCCAAATTGCTTCTGTCTGTTAAAT
GGAATTCTAGAGATGAAGTAGCCCAGATGTATTGCTTGGTAAAAGATTGG
CCTCCAATCAAACCTGAACAGGCTATGGAACTTCTGGACTGTAATTACCC
AGATCCTATGGTTCGAGGTTTTGCTGTTCGGTGCTTGGAAAAATATTTAA
CAGATGACAAACTTTCTCAGTATTTAATTCAGCTAGTACAGGTCCTAAAA
TATGAACAATATTTGGATAACTTGCTTGTGAGATTTTTACTGAAGAAAGC
ATTGACTAATCAAAGGATTGGGCACTTTTTCTTTTGGCATTTAAAATCTG
AGATGCACAATAAAACAGTTAGCCAGAGGTTTGGCCTGCTTTTGGAGTCC
TATTGTCGTGCATGTGGGATGTATTTGAAGCACCTGAATAGGCAAGTCGA
GGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCAAACAGGAGAAGA
AGGATGAAACACAAAAGGTACAGATGAAGTTTTTAGTTGAGCAAATGAGG
CGACCAGATTTCATGGATGCTCTACAGGGCTTTCTGTCTCCTCTAAACCC
TGCTCATCAACTAGGAAACCTCAGGCTTGAAGAGTGTCGAATTATGTCCT
CTGCAAAAAGGCCACTGTGGTTGAATTGGGAGAACCCAGACATCATGTCA
GAGTTACTGTTTCAGAACAATGAGATCATCTTTAAAAATGGGGATGATTT
ACGGCAAGATATGCTAACACTTCAAATTATTCGTATTATGGAAAATATCT
GGCAAAATCAAGGTCTTGATCTTCGAATGTTACCTTATGGTTGTCTGTCA
ATCGGTGACTGTGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACTAT
TATGCAAATTCAGTGCAAAGGCGGCTTGAAAGGTGCACTGCAGTTCAACA
GCCACACACTACATCAGTGGCTCAAAGACAAGAACAAAGGAGAAATATAT
GATGCAGCCATTGACCTGTTTACACGTTCATGTGCTGGATACTGTGTAGC
TACCTTCATTTTGGGAATTGGAGATCGTCACAATAGTAACATCATGGTGA
AAGACGATGGACAACTGTTTCATATAGATTTTGGACACTTTTTGGATCAC
AAGAAGAAAAAATTTGGTTATAAACGAGAACGTGTGCCATTTGTTTTGAC
ACAGGATTTCTTAATAGTGATTAGTAAAGGAGCCCAAGAATGCACAAAGA
CAAGAGAATTTGAGAGGTTTCAGGAGATGTGTTACAAGGCTTATCTAGCT
ATTCGACAGCATGCCAATCTCTTCATAAATCTTTTCTCAATGATGCTTGG
CTCTGGAATGCCAGAACTACAATCTTTTGATGACATTGCATACATTCGAA
AGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTTTGGAGTATTTCATG
AAACAAATGAATGATGCACATCATGGTGGCTGGACAACAAAAATGGATTG
GATCTTCCACACAATTAAACAGCATGCATTGAACTGAGGTACC


DNA sequence of insert 2 :
ggatccGAAACCATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTATAAAAAGGAAAGAGAAGAAGATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAAAGGGTCCTTAGTAGCTCTTGGATTCAGTGATGGACAGGAAGCCAGGCCTGAAGAAATTGGCTGGTTAAATGGCTATAATGAAACCACAGGGGAAAGGGGGGACTTTCCGGGAACTTACGTAGAATATATTGGAAGGAAAAAAATCTCGCCTCCCACACCAAAGCCCCGGCCACCTCGGCCTCTTCCTGTTGCACCAGGTTCTTCGAAAACTGAAGCAGATGTTGAACAACAAGCTTTGACTCTCCCGGATCTTGCAGAGCAGTTTGCCCCTCCTGACATTGCCCCGCCTCTTCTTATCAAGCTCGTGGAAGCCATTGAAAAGAAAGGTCTGGAATGTTCAACTCTATACAGAACACAGAGCTCCAGCAACCTGGCAGAATTACGACAGCTTCTTGATTGTGATACACCCTCCGTGGACTTGGAAATGATCGATGTGCACGTTTTGGCTGACGCTTTCAAACGCTATCTCCTGGACTTACCAAATCCTGTCATTCCAGCA
GCCGTTTACAGTGAAATGATTTCTTTAGCTCCAGAAGTACAAAGCTCCGAAGAATATATTCAGCTATTGAAGAAGCTTATTAGGTCGCCTAGCATACCTCATCAGTATTGGCTTACGCTTCAGTATTTGTTAAAACATTTCTTCAAGCTCTCTCAAACCTCCAGCAAAAATCTGTTGAATGCAAGAGTACTCTCTGAAATTTTCAGCCCTATGCTTTTCAGATTCTCAGCAGCCAGCTCTGATAATACTGAAAACCTCATAAAAGTTATAGAAATTTTAATCTCAACTGAATGGAATGAACGACAGCCTGCACCAGCACTGCCTCCTAAACCACCAAAACCTACTACTGTAGCCAACAACGGTATGAATAACAATATGTCCTTACAAGATGCTGAATGGTACTGGGGAGATATCTCGAGGGAAGAAGTGAATGAAAAACTTCGAGATACAGCAGACGGGACCTTTTTGGTACGAGATGCGTCTACTAAAATGCATGGTGATTATACTCTTACACTAAGGAAAGGGGGAAATAACAAATTAATCAAAATATTTCATCGAGATGGGAAATATGGCTTCTCTGACCCATTAACCTTCAGTTCTGTGGTTGAATTAATAAACCACTACCGGAATGAATCTCTAGCTCAGTATAATCCCAAATTGGATGTGAAATTACTTTATCCAGTATCCAAATACCAACAGGATCAAGTTGTCAAAGAAGATAATATTGAAGCTGTAGGGAAAAAATTACATGAATATAACACTCAGTTTCAAGAAAAAAGTCGAGAATATGATAGATTATATGAAGAATATACCCGCACATCCCAGGAAATCCAAATGAAAAGGACAGCTATTGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAGTGCCAGACCCAAGAGCGGTACAGCAAAGAATACATAGAAAAGTTTAAACGTGAAGGCAATGAGAAAGAAATACAAAGGATTATGCATAATTATGATAAGTTGAAGTCTCGAATCAGTGAAATTATTGACAGTAGAAGAAGATTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTATCGAGAAATTGACAAACGTATGAACAGCATTAAACCAGACCTTATCCAGCTGAGAAAGACGAGAGACCAATACTTGATGTGGTTGACTCAAAAAGGTGTTCGGCAAAAGAAGTTGAACGAGTGGTTGGGCAATGAAAACACTGAAGACCAATATTCACTGGTGGAAGATGATGAAGATTTGCCCCATCATGATGAGAAGACATGGAATGTTGGAAGCAGCAACCGAAACAAAGCTGAAAACCTGTTGCGAGGGAAGCGAGATGGCACTTTTCTTGTCCGGGAGAGCAGTAAACAGGGCTGCTATGCCTGCTCTGTAGTGGTGGACGGCGAAGTAAAGCATTGTGTCATAAACAAAACAGCAACTGGCTATGGCTTTGCCGAGCCCTATAACTTGTACAGCTCTCTGAAAGAACTGGTGCTACATTACCAACACACCTCCCTTGTGCAGCACAACGACTCCCTCAATGTCACACTAGCCTACCCAGTATATGCACAGCAGAGGCGATGAactagt

Amino Acid Sequence

Amino acid sequence of expressed protein 1 :
MHHHHHHPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLIT
IKHELFKEARKYPLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRL
FQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNIL
NVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVI
WVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVL
EYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLY
SQLPMDCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYV
NVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIP
DLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALN
LWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEE
HANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEIT
KQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIK
PEQAMELLDCNYPDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQY
LDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRA
CGMYLKHLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDF
MDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLF
QNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDC
VGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAI
DLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKK
FGYKRERVPFVLTQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQH
ANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMN
DAHHGGWTTKMDWIFHTIKQHALN*

Amino acid sequence of expressed protein 2 :
MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARP
EEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPG
SSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLY
RTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPA
AVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKL
SQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNE
RQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDT
ADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSS
VVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLH
EYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQT
QERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLK
KQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGN
ENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRE
SSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQH
TSLVQHNDSLNVTLAYPVYAQQRR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12812
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus dual expression plasmid to express 6his-tagged PI3K alpha and untagged p85.; Changes to PI3K alpha U79143 CDS: c222t, c879t, c1354t, g2784a (all silent). These changes present in EST clones covering these sequences and other PI3K alpha sequences e.g. AY335705. The sequence cloned in this construct is identical to that in all other DU PI3K alpha constructs.; ; Calc Mol weight of 6His PI3K alpha E545K : 125103 Daltons; Calc Mol Weight of p85 : 83596 Daltons