cDNA Clone PTEN

• Plasmid: pROEX PTEN G129E
• Parent Plasmid: pROEX
• Expressed: 6His-PTEN G129E
• Genbank: CR450306
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCGCCCTTCTCCCAGACATGACAGCCATCATCAAAGAGATCGTTAG
CAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAGACTTGACCT
ATATTTATCCAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAA
GGCGTATACAGGAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAA
GCATAAAAACCATTACAAGATATACAATCTTTGTGCTGAAAGACATTATG
ACACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTGAAGACCAT
AACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCA
ATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTAAAGCTG
GAAAGGAACGAACTGGTGTAATGATATGTGCATATTTATTACATCGGGGC
AAATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGAC
CAGAGACAAAAAGGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATT
ATTATAGCTACCTGTTAAAGAATCATCTGGATTATAGACCAGTGGCACTG
TTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAAC
TTGCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCT
CCAATTCAGGACCCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTC
CCTCAGCCGTTACCTGTGTGTGGTGATATCAAAGTAGAGTTCTTCCACAA
ACAGAACAAGATGCTAAAAAAGGACAAAATGTTTCACTTTTGGGTAAATA
CATTCTTCATACCAGGACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGA
AGTCTATGTGATCAAGAAATCGATAGCATTTGCAGTATAGAGCGTGCAGA
TAATGACAAGGAATATCTAGTACTTACTTTAACAAAAAATGATCTTGACA
AAGCAAATAAAGACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTG
AAGCTGTACTTCACAAAAACAGTAGAGGAGCCGTCAAATCCAGAGGCTAG
CAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAACCTGATCATT
ATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGAT
GAAGATCAGCATACACAAATTACAAAAGTCTGAAAGGGCGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFALLPDMTAIIKEIVSRNKRR
YQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHY
KIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSED
DNHVAAIHCKAGKERTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKG
VTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQF
VVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKML
KKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEY
LVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSV
TPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12745
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6 His for bacterial expression.; ; Estimated MW of expressed protein : 51462 Daltons