cDNA Clone PTEN

• Plasmid: pROEX PTEN C124S
• Parent Plasmid: pROEX
• Expressed: 6His-PTEN C124S
• Genbank: CR450306
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCGCCCTTCTCCCAGACATGACAGCCATCATCAAAGAGATCGTTAG
CAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAGACTTGACCT
ATATTTATCCAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAA
GGCGTATACAGGAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAA
GCATAAAAACCATTACAAGATATACAATCTTTGTGCTGAAAGACATTATG
ACACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTGAAGACCAT
AACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCA
ATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTCTAAAGCTG
GAAAGGGACGAACTGGTGTAATGATATGTGCATATTTATTACATCGGGGC
AAATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGAC
CAGAGACAAAAAGGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATT
ATTATAGCTACCTGTTAAAGAATCATCTGGATTATAGACCAGTGGCACTG
TTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAAC
TTGCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCT
CCAATTCAGGACCCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTC
CCTCAGCCGTTACCTGTGTGTGGTGATATCAAAGTAGAGTTCTTCCACAA
ACAGAACAAGATGCTAAAAAAGGACAAAATGTTTCACTTTTGGGTAAATA
CATTCTTCATACCAGGACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGA
AGTCTATGTGATCAAGAAATCGATAGCATTTGCAGTATAGAGCGTGCAGA
TAATGACAAGGAATATCTAGTACTTACTTTAACAAAAAATGATCTTGACA
AAGCAAATAAAGACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTG
AAGCTGTACTTCACAAAAACAGTAGAGGAGCCGTCAAATCCAGAGGCTAG
CAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAACCTGATCATT
ATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGAT
GAAGATCAGCATACACAAATTACAAAAGTCTGAAAGGGCGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFALLPDMTAIIKEIVSRNKRR
YQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHY
KIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSED
DNHVAAIHSKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKG
VTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQF
VVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKML
KKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEY
LVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSV
TPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12744
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6 His for bacterial expression.; ; Estimated MW of expressed protein : 51374 Daltons