cDNA Clone TBK1

• Plasmid: pCMV-HA-1 TBK1 1-687
• Parent Plasmid: pCMV5 HA1
• Expressed: HA TBK1 1-687
• Genbank: NP_037386
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTT
AGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTG
GTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCA
GTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAA
AAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGACATA
AAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTA
GAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGT
TTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAG
TGCACCGTGATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGAT
GGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAATTAGA
AGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACC
CTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATAT
GGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGC
TACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAG
AAGTGATGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGA
GTACAGAAAGCAGAAAATGGACCAATTGACTGGAGTGGAGACATGCCTGT
TTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTTCTTG
CAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTT
TTTGCAGAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTC
GCTACAACAAATGACAGCTCATAAGATTTATATACATAGCTATAATACTG
CTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCA
AATCAAGAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAG
GCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTTGTAG
TAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCC
CTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGC
TAAGGCAATAACAGGGGTTGTGTGTTATGCCTGCAGAATTGCCAGTACCT
TACTGCTTTATCAGGAATTAATGCGAAAGGGGATACGATGGCTGATTGAA
TTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGT
GATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTAT
ATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATT
TCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAAT
AGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCAC
TGGCAGACGCATGGGCACATCAAGAAGGCACTCATCCGAAAGACAGAAAT
GTAGAAAAACTACAAGTCCTGTTAAATTGCATGACAGAGATTTACTATCA
GTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAA
TCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATG
ACGCACTTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAA
GTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTATTAT
CGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAA
GAATATACTAATGAGTTACAAGAAACTCTGCCTCAGAAAATGTTTACAGC
TTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCTAACA
CATTAGTAGAAATGACTTGAGGTATGAAGAAATTAAAGGAAGAGATGGAA
GGGGTGGTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGG
CTCTTTAACCATGGATGGTGGCCTTCGCAACGTTGACTGTCTTTAGGCGG
CCGC

Amino Acid Sequence

MYPYDVPDYAGSMQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIK
VFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEF
CPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKP
GNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERA
VLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKII
TGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEAD
QEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHEL
VYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLN
TIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQEL
MRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKI
NLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAH
QEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQ
KLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCF
DIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12705
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminal HA tag; for mammalian expression.; ; Estimated MW of expressed protein : 80299 Daltons