cDNA Clone SMAD7

• Plasmid: pCMV-HA-1 SMAD7
• Parent Plasmid: pCMV5 HA1
• Expressed: HA SMAD7
• Genbank: O15105
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTTCAGGACCAAACGATCTGCGCTCGTCCGGCGTCTCTGGAG
GAGCCGTGCGCCCGGCGGCGAGGACGAGGAGGAGGGCGCAGGGGGAGGTG
GAGGAGGAGGCGAGCTGCGGGGAGAAGGGGCGACGGACAGCCGAGCGCAT
GGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGCTGCCTGGGCAAGGC
GGTGCGAGGTGCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCGGGCG
CCGGCGCGGCCGGGGGCGCCGAGGCGGATCTGAAGGCGCTCACGCACTCG
GTGCTCAAGAAACTGAAGGAGCGGCAGCTGGAGCTGCTGCTCCAGGCCGT
GGAGTCCCGCGGCGGGACGCGCACCGCGTGCCTCCTGCTGCCCGGCCGCC
TGGACTGCAGGCTGGGCCCGGGGGCGCCCGCCGGCGCGCAGCCTGCGCAG
CCGCCCTCGTCCTACTCGCTCCCCCTCCTGCTGTGCAAAGTGTTCAGGTG
GCCGGATCTCAGGCATTCCTCGGAAGTCAAGAGGCTGTGTTGCTGTGAAT
CTTACGGGAAGATCAACCCCGAGCTGGTGTGCTGCAACCCCCATCACCTT
AGCCGACTCTGCGAACTAGAGTCTCCCCCCCCTCCTTACTCCAGATACCC
GATGGATTTTCTCAAACCAACTGCAGACTGTCCAGATGCTGTGCCTTCCT
CCGCTGAAACAGGGGGAACGAATTATCTGGCCCCTGGGGGGCTTTCAGAT
TCCCAACTTCTTCTGGAGCCTGGGGATCGGTCACACTGGTGCGTGGTGGC
ATACTGGGAGGAGAAGACGAGAGTGGGGAGGCTCTACTGTGTCCAGGAGC
CCTCTCTGGATATCTTCTATGATCTACCTCAGGGGAATGGCTTTTGCCTC
GGACAGCTCAATTCGGACAACAAGAGTCAGCTGGTGCAGAAGGTGCGGAG
CAAAATCGGCTGCGGCATCCAGCTGACGCGGGAGGTGGATGGTGTGTGGG
TGTACAACCGCAGCAGTTACCCCATCTTCATCAAGTCCGCCACACTGGAC
AACCCGGACTCCAGGACGCTGTTGGTACACAAGGTGTTCCCCGGTTTCTC
CATCAAGGCTTTCGACTACGAGAAGGCGTACAGCCTGCAGCGGCCCAATG
ACCACGAGTTTATGCAGCAGCCGTGGACGGGCTTTACCGTGCAGATCAGC
TTTGTGAAGGGCTGGGGTCAGTGCTACACCCGCCAGTTCATCAGCAGCTG
CCCGTGCTGGCTAGAGGTCATCTTCAACAGCCGGTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMFRTKRSALVRRLWRSRAPGGEDEEEGAGGGGGGGELR
GEGATDSRAHGAGGGGPGRAGCCLGKAVRGAKGHHHPHPPAAGAGAAGGA
EADLKALTHSVLKKLKERQLELLLQAVESRGGTRTACLLLPGRLDCRLGP
GAPAGAQPAQPPSSYSLPLLLCKVFRWPDLRHSSEVKRLCCCESYGKINP
ELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGT
NYLAPGGLSDSQLLLEPGDRSHWCVVAYWEEKTRVGRLYCVQEPSLDIFY
DLPQGNGFCLGQLNSDNKSQLVQKVRSKIGCGIQLTREVDGVWVYNRSSY
PIFIKSATLDNPDSRTLLVHKVFPGFSIKAFDYEKAYSLQRPNDHEFMQQ
PWTGFTVQISFVKGWGQCYTRQFISSCPCWLEVIFNSR

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12468
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 47783 Daltons