cDNA Clone LIMK1

• Plasmid: pFBHTC LIMK1 T508D
• Parent Plasmid: pFastbac HTc
• Expressed: 6His LIMK1 T508D
• Genbank: P53667
• Tag: 6His

Sequence of Insert

GTCGACATGAGGTTGACGCTACTTTGTTGCACCTGGAGGGAAGAACGTAT
GGGAGAGGAAGGAAGCGAGTTGCCCGTGTGTGCAAGCTGCGGCCAGAGGA
TCTATGATGGCCAGTACCTCCAGGCCCTGAACGCGGACTGGCACGCAGAC
TGCTTCAGGTGTTGTGACTGCAGTGCCTCCCTGTCGCACCAGTACTATGA
GAAGGATGGGCAGCTCTTCTGCAAGAAGGACTACTGGGCCCGCTATGGCG
AGTCCTGCCATGGGTGCTCTGAGCAAATCACCAAGGGACTGGTTATGGTG
GCTGGGGAGCTGAAGTACCACCCCGAGTGTTTCATCTGCCTCACGTGTGG
GACCTTTATCGGTGACGGGGACACCTACACGCTGGTGGAGCACTCCAAGC
TGTACTGCGGGCACTGCTACTACCAGACTGTGGTGACCCCCGTCATCGAG
CAGATCCTGCCTGACTCCCCTGGCTCCCACCTGCCCCACACCGTCACCCT
GGTGTCCATCCCAGCCTCATCTCATGGCAAGCGTGGACTTTCAGTCTCCA
TTGACCCCCCGCACGGCCCACCGGGCTGTGGCACCGAGCACTCACACACC
GTCCGCGTCCAGGGAGTGGATCCGGGCTGCATGAGCCCAGATGTGAAGAA
TTCCATCCACGTCGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCC
GAAATGTGCCCCTGGACGAGATTGACCTGCTGATTCAGGAAACCAGCCGC
CTGCTCCAGCTGACCCTCGAGCATGACCCTCACGATACACTGGGCCACGG
GCTGGGGCCTGAGACCAGCCCCCTGAGCTCTCCGGCTTATACTCCCAGCG
GGGAGGCGGGCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGC
ATCGACAGGTCTCCGGGCGCTGGCTCACTGGGCTCCCCGGCCTCCCAGCG
CAAGGACCTGGGTCGCTCTGAGTCCCTCCGCGTAGTCTGCCGGCCACACC
GCATCTTCCGGCCGTCGGACCTCATCCACGGGGAGGTGCTGGGCAAGGGC
TGCTTCGGCCAGGCTATCAAGGTGACACACCGTGAGACAGGTGAGGTGAT
GGTGATGAAGGAGCTGATCCGGTTCGACGAGGAGACCCAGAGGACGTTCC
TCAAGGAGGTGAAGGTCATGCGATGCCTGGAACACCCCAACGTGCTCAAG
TTCATCGGGGTGCTCTACAAGGACAAGAGGCTCAACTTCATCACTGAGTA
CATCAAGGGCGGCACGCTCCGGGGCATCATCAAGAGCATGGACAGCCAGT
ACCCATGGAGCCAGAGAGTGAGCTTTGCCAAGGACATCGCATCAGGGATG
GCCTACCTCCACTCCATGAACATCATCCACCGAGACCTCAACTCCCACAA
CTGCCTGGTCCGCGAGAACAAGAATGTGGTGGTGGCTGACTTCGGGCTGG
CGCGTCTCATGGTGGACGAGAAGACTCAGCCTGAGGGCCTGCGGAGCCTC
AAGAAGCCAGACCGCAAGAAGCGCTACGACGTGGTGGGCAACCCCTACTG
GATGGCACCTGAGATGATCAACGGCCGCAGCTATGATGAGAAGGTGGATG
TGTTCTCCTTTGGGATCGTCCTGTGCGAGATCATCGGGCGGGTGAACGCA
GACCCTGACTACCTGCCCCGCACCATGGACTTTGGCCTCAACGTGCGAGG
ATTCCTGGACCGCTACTGCCCCCCAAACTGCCCCCCGAGCTTCTTCCCCA
TCACCGTGCGCTGTTGCGATCTGGACCCCGAGAAGAGGCCATCCTTTGTG
AAGCTGGAACACTGGCTGGAGACCCTCCGCATGCACCTGGCCGGCCACCT
GCCACTGGGCCCACAGCTGGAGCAGCTGGACAGAGGTTTCTGGGAGACCT
ACCGGCGCGGCGAGAGCGGACTGCCTGCCCACCCTGAGGTCCCCGACTGA
GCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDMRLTLLCCTWREER
MGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLSHQYY
EKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTC
GTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVT
LVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVK
NSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGH
GLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQ
RKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEV
MVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITE
YIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSH
NCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYDVVGNPY
WMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVR
GFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGH
LPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU12440
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 76929 Daltons