cDNA Clone BRAF

• Synonyms: NS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1
• Plasmid: pFastbac HTc BRAF
• Parent Plasmid: pFastbac HTc
• Expressed: 6His TEV BRAF
• Genbank: NP_004324.2
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACGCGGCGCTGAGCGGTGGCGGTGGTGGCGGCGCGGAGCCGGGCCA
GGCTCTGTTCAACGGGGACATGGAGCCCGAGGCCGGCGCCGGCGCCGGCG
CCGCGGCCTCTTCGGCTGCGGACCCTGCCATTCCGGAGGAGGTGTGGAAT
ATCAAACAAATGATTAAGTTGACACAGGAACATATAGAGGCCCTATTGGA
CAAATTTGGTGGGGAGCATAATCCACCATCAATATATCTGGAGGCCTATG
AAGAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTA
TTGGAATCTCTGGGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATC
AATGGATACCGTTACATCTTCTTCCTCTTCTAGCCTTTCAGTGCTACCTT
CATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCAACCCC
AAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG
GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGA
AAGCACTGATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGA
ATTCAGGATGGAGAGAAGAAACCAATTGGTTGGGACACTGATATTTCCTG
GCTTACTGGAGAAGAATTGCATGTGGAAGTGTTGGAGAATGTTCCACTTA
CAACACACAACTTTGTACGAAAAACGTTTTTCACCTTAGCATTTTGTGAC
TTTTGTCGAAAGCTGCTTTTCCAGGGTTTCCGCTGTCAAACATGTGGTTA
TAAATTTCACCAGCGTTGTAGTACAGAAGTTCCACTGATGTGTGTTAATT
ATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAACACCACCCA
ATACCACAGGAAGAGGCGTCCTTAGCAGAGACTGCCCTAACATCTGGATC
ATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTCACCA
GTCCGTCTCCTTCAAAATCCATTCCAATTCCACAGCCCTTCCGACCAGCA
GATGAAGATCATCGAAATCAATTTGGGCAACGAGACCGATCCTCATCAGC
TCCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGA
TTAGAGACCAAGGATTTCGTGGTGATGGAGGATCAACCACAGGTTTGTCT
GCTACCCCCCCTGCCTCATTACCTGGCTCACTAACTAACGTGAAAGCCTT
ACAGAAATCTCCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAG
AAGACAGGAATCGAATGAAAACACTTGGTAGACGGGACTCGAGTGATGAT
TGGGAGATTCCTGATGGGCAGATTACAGTGGGACAAAGAATTGGATCTGG
ATCATTTGGAACAGTCTACAAGGGAAAGTGGCATGGTGATGTGGCAGTGA
AAATGTTGAATGTGACAGCACCTACACCTCAGCAGTTACAAGCCTTCAAA
AATGAAGTAGGAGTACTCAGGAAAACACGACATGTGAATATCCTACTCTT
CATGGGCTATTCCACAAAGCCACAACTGGCTATTGTTACCCAGTGGTGTG
AGGGCTCCAGCTTGTATCACCATCTCCATATCATTGAGACCAAATTTGAG
ATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATGGATTA
CTTACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATAT
TTCTTCATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGCTACA
GTGAAATCTCGATGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATC
CATTTTGTGGATGGCACCAGAAGTCATCAGAATGCAAGATAAAAATCCAT
ACAGCTTTCAGTCAGATGTATATGCATTTGGGATTGTTCTGTATGAATTG
ATGACTGGACAGTTACCTTATTCAAACATCAACAACAGGGACCAGATAAT
TTTTATGGTGGGACGAGGATACCTGTCTCCAGATCTCAGTAAGGTACGGA
GTAACTGTCCAAAAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAG
AAAAGAGATGAGAGACCACTCTTTCCCCAAATTCTCGCCTCTATTGAGCT
GCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACCCTCCT
TGAATCGGGCTGGTTTCCAAACAGAGGATTTTAGTCTATATGCTTGTGCT
TCTCCAAAAACACCCATCCAGGCAGGGGGATATGGTGCGTTTCCTGTCCA
CTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALL
DKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQ
RTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCG
YKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSS
APNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGS
GSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMD
YLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQI
IFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPV
H*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbac HTc BRAF 191.35 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12430
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His N terminal fusion. ; ; Estimated MW of expressed protein : 88634 Daltons
• Low Temp: No