cDNA Clone MKK4

• Plasmid: pEBG6P-delta 1-35 MKK4
• Parent Plasmid: pGEX6P1
• Expressed: GST-MKK4 delta 1-39
• Genbank: BC060764
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAGCATGCAGGGTAAACGCAAAGCACTGAAGTTGAATTTTGCAAA
TCCACCTTTCAAATCTACAGCAAGGTTTACTCTGAATCCCAATCCTACAG
GAGTTCAAAACCCACACATAGAGAGACTGAGAACACACAGCATTGAGTCA
TCAGGAAAACTGAAGATCTCCCCTGAACAACACTGGGATTTCACTGCAGA
GGACTTGAAAGACCTTGGAGAAATTGGACGAGGAGCTTATGGTTCTGTCA
ACAAAATGGTCCACAAACCAAGTGGGCAAATAATGGCAGTTAAAAGAATT
CGGTCAACAGTGGATGAAAAAGAACAAAAACAACTTCTTATGGATTTGGA
TGTAGTAATGCGGAGTAGTGATTGCCCATACATTGTTCAGTTTTATGGTG
CACTCTTCAGAGAGGGTGACTGTTGGATCTGTATGGAACTCATGTCTACC
TCGTTTGATAAGTTTTACAAATATGTATATAGTGTATTAGATGATGTTAT
TCCAGAAGAAATTTTAGGCAAAATCACTTTAGCAACTGTGAAAGCACTAA
ACCACTTAAAAGAAAACTTGAAAATTATTCACAGAGATATCAAACCTTCC
AATATTCTTCTGGACAGAAGTGGAAATATTAAGCTCTGTGACTTCGGCAT
CAGTGGACAGCTTGTGGACTCTATTGCCAAGACAAGAGATGCTGGCTGTA
GGCCATACATGGCACCTGAAAGAATAGACCCAAGCGCATCACGACAAGGA
TATGATGTCCGCTCTGATGTCTGGAGTTTGGGGATCACATTGTATGAGTT
GGCCACAGGCCGATTTCCTTATCCAAAGTGGAATAGTGTATTTGATCAAC
TAACACAAGTCGTGAAAGGAGATCCTCCGCAGCTGAGTAATTCTGAGGAA
AGGGAATTCTCCCCGAGTTTCATCAACTTTGTCAACTTGTGCCTTACGAA
GGATGAATCCAAAAGGCCAAAGTATAAAGAGCTTCTGAAACATCCCTTTA
TTTTGATGTATGAAGAACGTGCCGTTGAGGTCGCATGCTATGTTTGTAAA
ATCCTGGATCAAATGCCAGCTACTCCCAGCTCTCCCATGTATGTCGATTG
AGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSSMQGKRKALKLNF
ANPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFT
AEDLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMD
LDVVMRSSDCPYIVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDD
VIPEEILGKITLATVKALNHLKENLKIIHRDIKPSNILLDRSGNIKLCDF
GISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVWSLGITLY
ELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCL
TKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYV
D*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12423
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag. ; ; Estimated MW of expressed protein : 68878 Daltons