cDNA Clone PI5P4 Kinase II alpha

• Plasmid: pET28a PI5P4 Kinase II alpha
• Parent Plasmid: pET28a original
• Expressed: 6His PI5P4 Kinase II alpha
• Genbank: NP_005019.2
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGACCCCCGGCAACCTAGGGTCCTCTGTCCTGGCGAGCAA
GACCAAGACCAAGAAGAAGCACTTCGTAGCGCAGAAAGTGAAGCTGTTTC
GGGCCAGCGACCCGCTGCTCAGCGTCCTCATGTGGGGGGTAAACCACTCG
ATCAATGAACTGAGCCATGTTCAAATCCCTGTTATGTTGATGCCAGATGA
CTTCAAAGCCTATTCAAAAATAAAGGTGGACAATCACCTTTTTAACAAAG
AAAACATGCCGAGCCATTTCAAGTTTAAGGAATACTGCCCGATGGTCTTC
CGTAACCTGCGGGAGAGGTTTGGAATTGATGATCAAGATTTCCAGAATTC
CCTGACCAGGAGCGCACCCCTCCCCAACGACTCCCAGGCCCGCAGTGGAG
CTCGTTTTCACACTTCCTACGACAAAAGATACATCATCAAGACTATTACC
AGTGAAGACGTGGCCGAAATGCACAACATCCTGAAGAAATACCACCAGTA
CATAGTGGAATGTCATGGGATCACCCTTCTTCCCCAGTTCTTGGGCATGT
ACCGGCTTAATGTTGATGGAGTTGAAATATATGTGATAGTTACAAGAAAT
GTATTCAGCCACCGTTTGTCTGTGTATAGGAAATACGACTTAAAGGGCTC
TACAGTGGCTAGAGAAGCTAGTGACAAAGAAAAGGCCAAAGAACTGCCAA
CTCTGAAAGATAATGATTTCATTAATGAGGGCCAAAAGATTTATATTGAT
GACAACAACAAGAAGGTCTTCCTGGAAAAACTAAAAAAGGATGTTGAGTT
TCTGGCCCAGCTGAAGCTCATGGACTACAGTCTGCTGGTGGGAATTCATG
ATGTGGAGAGAGCCGAACAGGAGGAAGTGGAGTGTGAGGAGAACGATGGG
GAGGAGGAGGGCGAGAGCGATGGCACCCACCCGGTGGGAACCCCCCCAGA
TAGCCCCGGGAATACACTGAACAGCTCACCACCCCTGGCTCCCGGGGAGT
TCGATCCGAACATCGACGTCTATGGAATTAAGTGCCATGAAAACTCGCCT
AGGAAGGAGGTGTACTTCATGGCAATTATTGACATCCTTACTCATTATGA
TGCAAAAAAGAAAGCTGCCCATGCTGCAAAAACTGTTAAACATGGCGCTG
GCGCGGAGATCTCCACCGTGAACCCAGAACAGTATTCAAAGCGCTTTTTG
GACTTTATTGGCCACATCTTGACGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMATPGNLGSSVLASKT
KTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDF
KAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSL
TRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYI
VECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGST
VAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFL
AQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDS
PGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDA
KKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU12296
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 49767 Daltons