cDNA Clone BRCA1

• Plasmid: pCMV-FLAG-3 BRCA1
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG-BRCA1
• Genbank: AAA73985.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACGGATCCATGGATTTATCTGCTCTTCGCGTTGAAGAAGTACAAAA
TGTCATTAATGCTATGCAGAAAATCTTAGAGTGTCCCATCTGTCTGGAGT
TGATCAAGGAACCTGTCTCCACAAAGTGTGACCACATATTTTGCAAATTT
TGCATGCTGAAACTTCTCAACCAGAAGAAAGGGCCTTCACAGTGTCCTTT
ATGTAAGAATGATATAACCAAAAGGAGCCTACAAGAAAGTACGAGATTTA
GTCAACTTGTTGAAGAGCTATTGAAAATCATTTGTGCTTTTCAGCTTGAC
ACAGGTTTGGAGTATGCAAACAGCTATAATTTTGCAAAAAAGGAAAATAA
CTCTCCTGAACATCTAAAAGATGAAGTTTCTATCATCCAAAGTATGGGCT
ACAGAAACCGTGCCAAAAGACTTCTACAGAGTGAACCCGAAAATCCTTCC
TTGCAGGAAACCAGTCTCAGTGTCCAACTCTCTAACCTTGGAACTGTGAG
AACTCTGAGGACAAAGCAGCGGATACAACCTCAAAAGACGTCTGTCTACA
TTGAATTGGGATCTGATTCTTCTGAAGATACCGTTAATAAGGCAACTTAT
TGCAGTGTGGGAGATCAAGAATTGTTACAAATCACCCCTCAAGGAACCAG
GGATGAAATCAGTTTGGATTCTGCAAAAAAGGCTGCTTGTGAATTTTCTG
AGACGGATGTAACAAATACTGAACATCATCAACCCAGTAATAATGATTTG
AACACCACTGAGAAGCGTGCAGCTGAGAGGCATCCAGAAAAGTATCAGGG
TAGTTCTGTTTCAAACTTGCATGTGGAGCCATGTGGCACAAATACTCATG
CCAGCTCATTACAGCATGAGAACAGCAGTTTATTACTCACTAAAGACAGA
ATGAATGTAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCTT
AGCAAGGAGCCAACATAACAGATGGGCTGGAAGTAAGGAAACATGTAATG
ATAGGCGGACTCCCAGCACAGAAAAAAAGGTAGATCTGAATGCTGATCCC
CTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTCAGAGAA
TCCTAGAGATACTGAAGATGTTCCTTGGATAACACTAAATAGCAGCATTC
AGAAAGTTAATGAGTGGTTTTCCAGAAGTGATGAACTGTTAGGTTCTGAT
GACTCACATGATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATT
GGACGTTCTAAATGAGGTAGATGAATATTCTGGTTCTTCAGAGAAAATAG
ACTTACTGGCCAGTGATCCTCATGAGGCTTTAATATGTAAAAGTGAAAGA
GTTCACTCCAAATCAGTAGAGAGTAATATTGAAGACAAAATATTTGGGAA
AACCTATCGGAAGAAGGCAAGCCTCCCCAACTTAAGCCATGTAACTGAAA
ATCTAATTATAGGAGCATTTGTTACTGAGCCACAGATAATACAAGAGCGT
CCCCTCACAAATAAATTAAAGCGTAAAAGGAGACCTACATCAGGCCTTCA
TCCTGAGGATTTTATCAAGAAAGCAGATTTGGCAGTTCAAAAGACTCCTG
AAATGATAAATCAGGGAACTAACCAAACGGAGCAGAATGGTCAAGTGATG
AATATTACTAATAGTGGTCATGAGAATAAAACAAAAGGTGATTCTATTCA
GAATGAGAAAAATCCTAACCCAATAGAATCACTCGAAAAAGAATCTGCTT
TCAAAACGAAAGCTGAACCTATAAGCAGCAGTATAAGCAATATGGAACTC
GAATTAAATATCCACAATTCAAAAGCACCTAAAAAGAATAGGCTGAGGAG
GAAGTCTTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGTAGAA
ATCTAAGCCCACCTAATTGTACTGAATTGCAAATTGATAGTTGTTCTAGC
AGTGAAGAGATAAAGAAAAAAAAGTACAACCAAATGCCAGTCAGGCACAG
CAGAAACCTACAACTCATGGAAGGTAAAGAACCTGCAACTGGAGCCAAGA
AGAGTAACAAGCCAAATGAACAGACAAGTAAAAGACATGACAGTGATACT
TTCCCAGAGCTGAAGTTAACAAATGCACCTGGTTCTTTTACTAAGTGTTC
AAATACCAGTGAACTTAAAGAATTTGTCAATCCTAGCCTTCCAAGAGAAG
AAAAAGAAGAGAAACTAGAAACAGTTAAAGTGTCTAATAATGCTGAAGAC
CCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAACTGAAAGATC
TGTAGAGAGTAGCAGTATTTCACTGGTACCTGGTACTGATTATGGCACTC
AGGAAAGTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGCAAAAACA
GAACCAAATAAATGTGTGAGTCAGTGTGCAGCATTTGAAAACCCCAAGGG
ACTAATTCATGGTTGTTCCAAAGATAATAGAAATGACACAGAAGGCTTTA
AGTATCCATTGGGACATGAAGTTAACCACAGTCGGGAAACAAGCATAGAA
ATGGAAGAAAGTGAACTCGATGCTCAGTATTTGCAGAATACATTCAAGGT
TTCAAAGCGCCAGTCATTTGCTCTGTTTTCAAATCCAGGAAATGCAGAAG
AGGAATGTGCAACATTCTCTGCCCACTCTGGGTCCTTAAAGAAACAAAGT
CCAAAAGTCACTTTTGAATGTGAACAAAAGGAAGAAAATCAAGGAAAGAA
TGAGTCTAATATCAAGCCTGTACAGACAGTTAATATCACTGCAGGCTTTC
CTGTGGTTGGTCAGAAAGATAAGCCAGTTGATAATGCCAAATGTAGTATC
AAAGGAGGCTCTAGGTTTTGTCTATCATCTCAGTTCAGAGGCAACGAAAC
TGGACTCATTACTCCAAATAAACATGGACTTTTACAAAACCCATATCGTA
TACCACCACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAAGAAA
AATCTGCTAGAGGAAAACTTTGAGGAACATTCAATGTCACCTGAAAGAGA
AATGGGAAATGAGAACATTCCAAGTACAGTGAGCACAATTAGCCGTAATA
ACATTAGAGAAAATGTTTTTAAAGGAGCCAGCTCAAGCAATATTAATGAA
GTAGGTTCCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTC
CAGTGATGAAAACATTCAAGCAGAACTAGGTAGAAACAGAGGGCCAAAAT
TGAATGCTATGCTTAGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAA
AGTCTTCCTGGAAGTAATTGTAAGCATCCTGAAATAAAAAAGCAAGAATA
TGAAGAAGTAGTTCAGACTGTTAATACAGATTTCTCTCCATATCTGATTT
CAGATAACTTAGAACAGCCTATGGGAAGTAGTCATGCATCTCAGGTTTGT
TCTGAGACACCTGATGACCTGTTAGATGATGGTGAAATAAAGGAAGATAC
TAGTTTTGCTGAAAATGACATTAAGGAAAGTTCTGCTGTTTTTAGCAAAA
GCGTCCAGAGAGGAGAGCTTAGCAGGAGTCCTAGCCCTTTCACCCATACA
CATTTGGCTCAGGGTTACCGAAGAGGGGCCAAGAAATTAGAGTCCTCAGA
AGAGAACTTATCTAGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGT
TATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACTAGGCATAGCACC
GTTGCTACCGAGTGTCTGTCTAAGAACACAGAGGAGAATTTATTATCATT
GAAGAATAGCTTAAATGACTGCAGTAACCAGGTAATATTGGCAAAGGCAT
CTCAGGAACATCACCTTAGTGAGGAAACAAAATGTTCTGCTAGCTTGTTT
TCTTCACAGTGCAGTGAATTGGAAGACTTGACTGCAAATACAAACACCCA
GGATCCTTTCTTGATTGGTTCTTCCAAACAAATGAGGCATCAGTCTGAAA
GCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGAAGAA
AGAGGAACGGGCTTGGAAGAAAATAATCAAGAAGAGCAAAGCATGGATTC
AAACTTAGGTGAAGCAGCATCTGGGTGTGAGAGTGAAACAAGCGTCTCTG
AAGACTGCTCAGGGCTATCCTCTCAGAGTGACATTTTAACCACTCAGCAG
AGGGATACCATGCAACATAACCTGATAAAGCTCCAGCAGGAAATGGCTGA
ACTAGAAGCTGTGTTAGAACAGCATGGGAGCCAGCCTTCTAACAGCTACC
CTTCCATCATAAGTGACTCCTCTGCCCTTGAGGACCTGCGAAATCCAGAA
CAAAGCACATCAGAAAAAGATTCGCATATACATGGCCAAAGGGACAACTC
CATGTTTTCTAAAAGGCCTAGAGAACATATATCAGTATTAACTTCACAGA
AAAGTAGTGAATACCCTATAAGCCAGAATCCAGAAGGCCTTTCTGCTGAC
AAGTTTGAGGTGTCTGCAGATAGTTCTACCAGTAAAAATAAAGAACCAGG
AGTGGAAAGGTCATCCCCTTCTAAATGCCCATCATTAGATGATAGGTGGT
ACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAA
GAGGAGCTCATTAAGGTTGTTGATGTGGAGGAGCAACAGCTGGAAGAGTC
TGGGCCACACGATTTGACGGAAACATCTTACTTGCCAAGGCAAGATCTAG
AGGGAACCCCTTACCTGGAATCTGGAATCAGCCTCTTCTCTGATGACCCT
GAATCTGATCCTTCTGAAGACAGAGCCCCAGAGTCAGCTCGTGTTGGCAA
CATACCATCTTCAACCTCTGCATTGAAATTTCCCCAATTGAAAGTTGCAG
AATCTGCCCAGGGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTAT
AATGCAATGGAAGAAAGTGTGAGCAGGGAGAAGCCAGAATTGACAGCTTC
AACAGAAAGGGTCAACAAAAGAATGTCCATGGTGGTGTCTGGCCTGACCC
CAGAAGAATTTATGCTCGTGTACAAGTTTGCCAGAAAACACCACATCACT
TTAACTAATCTAATTACTGAAGAGACTACTCATGTTGTTATGAAAACAGA
TGCTGAGTTTGTGTGTGAACGGACACTGAAATATTTTCTAGGAATTGCGG
GAGGAAAATGGGTAGTTAGCTATTTCTGGGTGACCCAGTCTATTAAAGAA
AGAAAAATGCTGAATGAGCATGATTTTGAAGTCAGAGGAGATGTGGTCAA
TGGAAGAAACCACCAAGGTCCAAAGCGAGCAAGAGAATCCCAGGACAGAA
AGATCTTCAGGGGGCTAGAAATCTGTTGCTATGGGCCCTTCACCAACATG
CCCACAGATCAACTGGAATGGATGGTACAGCTGTGTGGTGCTTCTGTGGT
GAAGGAGCTTTCATCATTCACCCTTGGCACAGGTGTCCACCCAATTGTGG
TTGTGCAGCCAGATGCCTGGACAGAGGACAATGGCTTCCATGCAATTGGG
CAGATGTGTGAGGCACCTGTGGTGACCCGAGAGTGGGTGTTGGACAGTGT
AGCACTCTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCC
CCCACAGCCACTACTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDGSMDLSALRVEEVQNVINAMQKILECPICLEL
IKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFS
QLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGY
RNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYI
ELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSE
TDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHA
SSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCND
RRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQ
KVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKID
LLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTEN
LIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPE
MINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAF
KTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRN
LSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKK
SNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREE
KEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQ
ESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFK
YPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFALFSNPGNAEE
ECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFP
VVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRI
PPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNN
IRENVFKGASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKL
NAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLIS
DNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKS
VQRGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLL
FGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKAS
QEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSES
QGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSE
DCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYP
SIISDSSALEDLRNPEQSTSEKDSHIHGQRDNSMFSKRPREHISVLTSQK
SSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWY
MHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLE
GTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKFPQLKVAE
SAQGPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTP
EEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAG
GKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRK
IFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVV
VQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIP
HSHY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12254
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: General Description PCRed and then corrected. Differs to genbank at E1038G, K1183R, V1602F, S1613G and has a 22 amino acid insert between 1452 and 1453. The point mutations are common (non disease causing) SNPs.; ; Estimated MW of expressed protein : 212519 Daltons