cDNA Clone PI5P4 Kinase II alpha

• Plasmid: pGEX6P-1-PI5P4 Kinase II alpha
• Parent Plasmid: pGEX6P1
• Expressed: GST-PI5P4 Kinase II alpha
• Genbank: NP_005019.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGACCCCCGGCAACCTAGGGTCCTCTGTCCTGGCGAGCAA
GACCAAGACCAAGAAGAAGCACTTCGTAGCGCAGAAAGTGAAGCTGTTTC
GGGCCAGCGACCCGCTGCTCAGCGTCCTCATGTGGGGGGTAAACCACTCG
ATCAATGAACTGAGCCATGTTCAAATCCCTGTTATGTTGATGCCAGATGA
CTTCAAAGCCTATTCAAAAATAAAGGTGGACAATCACCTTTTTAACAAAG
AAAACATGCCGAGCCATTTCAAGTTTAAGGAATACTGCCCGATGGTCTTC
CGTAACCTGCGGGAGAGGTTTGGAATTGATGATCAAGATTTCCAGAATTC
CCTGACCAGGAGCGCACCCCTCCCCAACGACTCCCAGGCCCGCAGTGGAG
CTCGTTTTCACACTTCCTACGACAAAAGATACATCATCAAGACTATTACC
AGTGAAGACGTGGCCGAAATGCACAACATCCTGAAGAAATACCACCAGTA
CATAGTGGAATGTCATGGGATCACCCTTCTTCCCCAGTTCTTGGGCATGT
ACCGGCTTAATGTTGATGGAGTTGAAATATATGTGATAGTTACAAGAAAT
GTATTCAGCCACCGTTTGTCTGTGTATAGGAAATACGACTTAAAGGGCTC
TACAGTGGCTAGAGAAGCTAGTGACAAAGAAAAGGCCAAAGAACTGCCAA
CTCTGAAAGATAATGATTTCATTAATGAGGGCCAAAAGATTTATATTGAT
GACAACAACAAGAAGGTCTTCCTGGAAAAACTAAAAAAGGATGTTGAGTT
TCTGGCCCAGCTGAAGCTCATGGACTACAGTCTGCTGGTGGGAATTCATG
ATGTGGAGAGAGCCGAACAGGAGGAAGTGGAGTGTGAGGAGAACGATGGG
GAGGAGGAGGGCGAGAGCGATGGCACCCACCCGGTGGGAACCCCCCCAGA
TAGCCCCGGGAATACACTGAACAGCTCACCACCCCTGGCTCCCGGGGAGT
TCGATCCGAACATCGACGTCTATGGAATTAAGTGCCATGAAAACTCGCCT
AGGAAGGAGGTGTACTTCATGGCAATTATTGACATCCTTACTCATTATGA
TGCAAAAAAGAAAGCTGCCCATGCTGCAAAAACTGTTAAACATGGCGCTG
GCGCGGAGATCTCCACCGTGAACCCAGAACAGTATTCAAAGCGCTTTTTG
GACTTTATTGGCCACATCTTGACGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATPGNLGSSVLASKTKTK
KKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAY
SKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRS
APLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVEC
HGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAR
EASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQL
KLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGN
TLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKK
AAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12243
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 73046 Daltons