cDNA Clone Ubiquitin

• Plasmid: pGEX6P-1-FLAG Ubiquitin K11wt
• Parent Plasmid: pGEX6P1
• Expressed: GST-FLAG Ubiquitin K11wt
• Genbank: P62988
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCCTTGCAGATCTGCCACCATGGACTACAAGGACGACGATGAC
AAGCAAATCTTCGTGAGGACCCTGACTGGTAAGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGACAGGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIRPCRSATMDYKDDDDK
QIFVRTLTGKTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLE
DGRTLSDYNIQRESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12118
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/FLAG for bacterial expression.