cDNA Clone TAU

• Plasmid: pCMV-HA Tau T50E
• Parent Plasmid: pCMV5
• Expressed: HA-Tau T50E
• Genbank: NP_005901.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCTGAGCC
CCGCCAGGAGTTCGAAGTGATGGAAGATCACGCTGGGACGTACGGGTTGG
GGGACAGGAAAGATCAGGGGGGCTACACCATGCACCAAGACCAAGAGGGT
GACACGGACGCTGGCCTGAAAGAATCTCCCCTGCAGGAACCCACTGAGGA
CGGATCTGAGGAACCGGGCTCTGAAACCTCTGATGCTAAGAGCACTCCAA
CAGCGGAAGATGTGACAGCACCCTTAGTGGATGAGGGAGCTCCCGGCAAG
CAGGCTGCCGCGCAGCCCCACACGGAGATCCCAGAAGGAACCACAGCTGA
AGAAGCAGGCATTGGAGACACCCCCAGCCTGGAAGACGAAGCTGCTGGTC
ACGTGACCCAAGCTCGCATGGTCAGTAAAAGCAAAGACGGGACTGGAAGC
GATGACAAAAAAGCCAAGGGGGCTGATGGTAAAACGAAGATCGCCACACC
GCGGGGAGCAGCCCCTCCAGGCCAGAAGGGCCAGGCCAACGCCACCAGGA
TTCCAGCAAAAACCCCGCCCGCTCCAAAGACACCACCCAGCTCTGGTGAA
CCTCCAAAATCAGGGGATCGCAGCGGCTACAGCAGCCCCGGCTCCCCAGG
CACTCCCGGCAGCCGCTCCCGCACCCCGTCCCTTCCAACCCCACCCACCC
GGGAGCCCAAGAAGGTGGCAGTGGTCCGTACTCCACCCAAGTCGCCGTCT
TCCGCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGACCTGAA
GAATGTCAAGTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGG
GAGGCGGGAAGGTGCAGATAATTAATAAGAAGCTGGATCTTAGCAACGTC
CAGTCCAAGTGTGGCTCAAAGGATAATATCAAACACGTCCCGGGAGGCGG
CAGTGTGCAAATAGTCTACAAACCAGTTGACCTGAGCAAGGTGACCTCCA
AGTGTGGCTCATTAGGCAACATCCATCATAAACCAGGAGGTGGCCAGGTG
GAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGAGTCCAGTCGAAGAT
TGGGTCCCTGGACAATATCACCCACGTCCCTGGCGGAGGAAATAAAAAGA
TTGAAACCCACAAGCTGACCTTCCGCGAGAACGCCAAAGCCAAGACAGAC
CACGGGGCGGAGATCGTGTACAAGTCGCCAGTGGTGTCTGGGGACACGTC
TCCACGGCATCTCAGCAATGTCTCCTCCACCGGCAGCATCGACATGGTAG
ACTCGCCCCAGCTCGCCACGCTAGCTGACGAGGTGTCTGCCTCCCTGGCC
AAGCAGGGTTTGTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDA
GLKESPLQEPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAA
QPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKK
AKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKS
GDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKS
RLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKC
GSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKS
EKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAE
IVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12015
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 46960 Daltons