cDNA Clone TCII

• Plasmid: pGEX6P-1-TCII 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-TCII 6His
• Genbank: CAK54921.1
• Tag: GST

Sequence of Insert

GGATCCATGAGGCACCTTGGGGCCTTCCTCTTCCTTCTGGGGGTCCTGGG
GGCCCTCACTGAGATGTGTGAAATACCAGAGATGGACAGCCATCTGGTAG
AGAAGTTGGGCCAGCACCTCTTACCTTGGATGGACCGGCTTTCCCTGGAG
CACTTGAACCCCAGCATCTATGTGGGCCTACGCCTCTCCAGTCTGCAGGC
TGGGACCAAGGAAGACCTCTACCTGCACAGCCTCAAGCTTGGTTACCAGC
AGTGCCTCCTAGGGTCTGCCTTCAGCGAGGATGACAGTGACTGCCAGGGC
AAGCCTTCCATGGGCCAGCTGGCCCTCTACCTGCTCGCTCTCAGAGCCAA
CTGTGAGTTTGTCAGGGGCCACAAGGGGGACAGGCTGGTCTCACAGCTCA
AATGGTTCCTGGAGGATGAGAAGAGAGCCATTGGGCATGATCACAAGGGC
CACCCCCACACTAGCTACTACCAGTATGGCCTGGGCATTCTGGCCCTGTG
TCTCCACCAGAAGCGGGTCCATGACAGCGTGGTGGACAAACTTCTGTATG
CTGTGGAACCTTTCCACCAGGGCCACCATTCTGTGGACACAGCAGCCATG
GCAGGCTTGGCATTCACCTGTCTGAAGCGCTCAAACTTCAACCCTGGTCG
GAGACAACGGATCACCATGGCCATCAGAACAGTGCGAGAGGAGATCTTGA
AGGCCCAGACCCCCGAGGGCCACTTTGGGAATGTCTACAGCACCCCATTG
GCATTACAGTTCCTCATGACTTCCCCCATGCCTGGGGCAGAACTGGGAAC
AGCATGTCTCAAGGCGAGGGTTGCTTTGCTGGCCAGTCTGCAGGATGGAG
CCTTCCAGAATGCTCTCATGATTTCCCAGCTGCTGCCCGTTCTGAACCAC
AAGACCTACATTGATCTGATCTTCCCAGACTGTCTGGCACCACGAGTCAT
GTTGGAACCAGCTGCTGAGACCATTCCTCAGACCCAAGAGATCATCAGTG
TCACGCTGCAGGTGCTTAGTCTCTTGCCGCCGTACAGACAGTCCATCTCT
GTTCTGGCCGGGTCCACCGTGGAAGATGTCCTGAAGAAGGCCCATGAGTT
AGGAGGATTCACATATGAAACACAGGCCTCCTTGTCAGGCCCCTACTTAA
CCTCCGTGATGGGGAAAGCGGCCGGAGAAAGGGAGTTCTGGCAGCTTCTC
CGAGACCCCAACACCCCACTGTTGCAAGGTATTGCTGACTACAGACCCAA
GGATGGAGAAACCATTGAGCTGAGGCTGGTTAGCTGGCATCATCACCATC
ACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMRHLGAFLFLLGVLGALTE
MCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKE
DLYLHSLKLGYQQCLLGSAFSEDDSDCQGKPSMGQLALYLLALRANCEFV
RGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQK
RVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRI
TMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMPGAELGTACLK
ARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPA
AETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFT
YETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGET
IELRLVSWHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8992
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST, C ter 6His for bacterial expression. Differs to genbank at G94S. (as supplied by external customer); ; Estimated MW of expressed protein : 75150 Daltons